A low-density breeding method for maintaining the wingless state of the pea aphid parthenogenes
A breeding method and low-density technology, applied in leguminous plant cultivation, animal husbandry and other directions, can solve problems such as difficult to achieve breeding, and achieve the effects of no breeding, stable state and simple operation.
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[0015] The present invention will be further described below by specific examples.
[0016] A low-density breeding method for maintaining the wingless state of the pea aphid parthenosexual, specifically comprising the following steps:
[0017] (1) Broad bean planting: Soak the broad bean seeds in warm water for 12 hours, and set aside; mix the Northeast black soil and vermiculite at a volume ratio of 1:2, add water and stir evenly, and move it into a flowerpot to lay flat, and put the broad bean seeds Insert it into the cultivation soil, water until the substrate in the flower pot is soaked, place it under the condition of light of 16L:8D, temperature of 28°C, and humidity of 60%, and water once every two days;
[0018] (2) Seedling transplantation and inoculation: 7-8 days after sowing, when the broad bean grows to 5-8cm, that is, when two true leaves are unfolded, move the broad bean plant into a plastic cup, cut a small hole in the filter paper, and make the broad bean stem...
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