Biological assaying method for evaluating effect of transgene cotton on chrysopa pallens

A bioassay, lacewing technology, applied in biological testing, material inspection products, etc., can solve problems such as the narrow scope of action of insect-resistant genetically modified crops, and achieve the effect of wide application and strong practicability

Active Publication Date: 2014-11-05
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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Problems solved by technology

Yu et al. also believe that the currently used insect-resistant transgenic crops have a narrow range of action, have no direct adverse

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  • Biological assaying method for evaluating effect of transgene cotton on chrysopa pallens

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Embodiment 1

[0038] The 2% agar solution obtained by mixing the agar powder and distilled water was heated and boiled, and the hot agar solution was poured into a disposable plastic petri dish and cooled to room temperature; Insert the newly hatched Cry1Ac-resistant or sensitive cotton bollworm larvae, and tighten the culture dish with a rubber ring to prevent the newly hatched larvae from escaping.

[0039] Cotton varieties: transgenic cotton 33B (expressing Cry1Ac protein), transgenic cotton variety Bollgard II (expressing Cry1Ac+Cry2Ab), non-transgenic cotton parent 33. Cotton bollworm strains: Cry1Ac-resistant strains (resistance strains of cotton bollworms screened indoors with Cry1Ac protein), sensitive cotton bollworms.

[0040] Feed the newly hatched larvae with pea aphids for 2 days, then feed the larvae with cotton bollworm larvae that have eaten transgenic cotton (33B, Bollgard II) for 2 days, and replace with new larvae every day until the larvae eat cotton bolls After 5 days ...

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Abstract

The invention provides a biological assaying method for evaluating the effect of transgene cotton on chrysopa pallens. The method comprises the following steps: paving agar on the bottom of a culture dish; placing transgene cotton leaves on the surface of the agar, inoculating newly-hatched helicoverpa armigera larvae; feeding newly-hatched chrysopa pallens larvae for 1 to 3 days with pea aphid, then feeding the chrysopa pallens larvae with the helicoverpa armigera larvae which has been fed by the transgene cotton leaves for 1 to 3 days; observing the growth situation of the chrysopa pallens larvae since the first day when the chrysopa pallens larvae is fed with the helicoverpa armigera larvae until the chrysopa pallens larvae pupate and perform eclosion; setting a reference group; comparing the differences of the experiment group and the reference group on the indexes of pupation rate, eclosion rate, larva weight, imago weight, and total growth time, and analyzing the difference significance through variance analysis so as to evaluate the effect of transgene cotton on chrysopa pallens. The application range of the biological assaying method is wide. The effect of high dose protein in transgene plants on predators can be effectively measured by utilizing the nutrition relationship evaluation of transgene plant-pest-predator.

Description

technical field [0001] The invention relates to the technical field of plant protection, in particular to a bioassay method for evaluating the influence of transgenic cotton on the growth and development of lacewings by using Bt-resistant cotton bollworms. Background technique [0002] The large lacewing Chrysopa pallens belongs to the class Insecta, order Neuroptera, and family Lacewingidae. It is a ubiquitous and resource-rich natural enemy in many crop ecosystems. Both its larvae and adults are predatory, and it can feed on aphids, spider mites, Lepidoptera eggs and young larvae, etc., and has a wide range of predation. Early people evaluated the impact of transgenic plants on lacewings, and the results showed that transgenic plants had no adverse effects on lacewings. For example, Li et al. found that after common lacewings ate corn pollen expressing Cry1Ab or Cry3Bb1, their survival rate, pre-oviposition period, Fecundity, egg production and adult dry weight were not s...

Claims

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Application Information

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IPC IPC(8): G01N33/48
Inventor 王英丽梁革梅魏纪珍袁向东赵曼
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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