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A kind of method for purifying and propagating ladybug

A kind of technology of mole-shaped ladybug and larva, which is applied in the field of artificial purification and multiplication of mole-shaped ladybug, can solve the problems of manual purification trouble, unfavorable egg retrieval and egg collection, etc. easy effect

Active Publication Date: 2021-01-01
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When rearing indoors, the lady beetle will lay eggs on the stems, leaves, and walls of the insect cage, which will bring some troubles to its artificial purification, and these places are not conducive to artificial egg retrieval and egg pairing during experiments. The problem of collecting and collecting eggs needs to be solved urgently

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The indoor artificial rearing method (purification+propagation) of embodiment 1 moire ladybug

[0026] 1. Catch ladybugs from the field and put them in insect cages (called No. 1 insect cage, and the ladybugs in this insect cage are called the first generation of ladybugs). There are broad bean seedlings (planted in small flowerpots) connected with enough pea aphids. The height of the broad bean seedlings should be 9-17cm as much as possible, and the number of stretched leaves should be as few as possible. Wrap a circle of wet plastic wrap around the small planter. Change the plastic wrap at 9:00, 15:00, and 21:00. Cut the egg mass from the replaced plastic wrap and put it in a fresh-keeping box covered with wet gauze. The feeding conditions of the tortoise beetle are temperature 25±1°C, humidity 70±10%, and photoperiod 16L:8D.

[0027] 2. The hatched larvae are picked out and placed in a new insect cage, which also has broad bean seedlings connected with pea aphids ...

Embodiment 2

[0031] Embodiment 2 increases the test of humidity hatching ovum

[0032] 1. At 24:00, preservative film is wrapped on the small flowerpot of broad bean sprouts in the No. 5 insect cage of embodiment 1, and the eggs are collected, and the preservative film is collected at 7:50 the next morning, and the egg masses are cut off. Place them in culture dishes with two kinds of humidity respectively. One culture dish is covered with wet gauze at the bottom (placed vertically, no water drops will drip down for 20 seconds), and the other culture dish has no wet gauze. A total of 6 days of eggs were collected, 6 groups of experiments (January 8th to January 13th, 2017).

[0033] 2. Observe the start time of hatching of 6 groups of test eggs, and take the time when more than half of the larvae have just hatched as the hatching time.

[0034] Table 1 Comparison of hatching time under different humidity

[0035] humidity Experiment 1 Experiment 2 Experiment 3 Experiment...

Embodiment 3

[0038] The test of embodiment 3 preservative film collecting ovum method

[0039] Carry out the collection of ovum with preservative film, filter paper, broad bean blade in No. 5 insect cages of embodiment 1, and filter paper is circular diameter 15cm, close to be placed on the side of planting broad bean seedling container in the insect cage, about 50 in the cage First adult (31 females). Subject to three days. Check the amount of eggs collected each day. The time is from December 7th to December 9th, 2016.

[0040] Table 2 Egg quantity (grain) of different devices

[0041] device December 7th December 8 December 9th plastic wrap 275 217 258 filter paper 0 0 7 broad bean leaves 183 121 153

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PUM

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Abstract

The invention provides a purification and expanding propagation method for propylaea japonica. The method comprises the steps that 1, the propylaea japonica is fed in an insect feeding cage, wherein horsebean seedlings are planted and enough pea aphids are fed in the insect feeding cage, a flowerpot of horsebean seedling planting is wrapped with a preservative film, and the number of the stretching blades of the horsebean seedlings is as little as possible so that most of spawn is spawned on the preservative film; 2, the spawned preservative film is collected, and larvae are hatched in a warm and humid condition; 3, the larvae are taken out in time and put into another insect feeding cage to be fed, after the larvae pupate, the spawn collecting operation in the step 2 is stopped; 4, after the spawn in another insect feeding cage in the step 3 is collected through the preservative film, the step 2, the step 3 and the step 4 are repeated until five generations, and the purified spawn is obtained; 5, after the purified spawn is hatched, expanding propagation in the insect feeding cage is conducted. The purification and expanding propagation method is beneficial to purify the generation number of propylaea japonica in the field and reduce newly hatched larvae and imagoes feeding of the spawn indoors, the operation is easy, the cost is low, and purification and expanding propagation for propylaea japonica indoors can be completed effectively.

Description

technical field [0001] The invention relates to a method for artificially purifying, multiplying and breeding the ladybug, in particular to an artificial indoor breeding method for the ladybug. Background technique [0002] The tortoise beetle belongs to the Coleoptera insects, and it is one of the main natural enemy insects in the field. Both its adults and larvae can prey on aphids, thrips, spider mites, eggs and young larvae of Lepidoptera. Therefore, when the number of ladybugs reaches a certain amount, the number of field aphids and some small pests can be well controlled, and crops can be protected to a certain extent. According to the predation characteristics of the ladybug, it can be considered to use the ladybug in the biological control of field pests to achieve the purpose of integrated pest management in the field. Now there are a large number of literatures reporting articles about the predation characteristics of M. mori, indoor artificial feeding of M. molyb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 崔金杰吴林珂张帅雒珺瑜
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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