Allergic rhinitis animal model construction method

A technology for allergic rhinitis and animal models, which can be used in pharmaceutical formulations, compound screening/testing, preparations for in vivo experiments, etc. It can solve the problems of non-uniformity, great differences in optimal conditions for sensitization and stimulation, and achieve Low cost, reduced allergen usage, and waste-free effects

Pending Publication Date: 2017-09-12
ZONHON BIOPHARMA INST
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0006] Due to the differences in the immunogenicity and other properties of different allergens, different animals have different AR symptoms after sensitization, so the method of using different allergens to construct AR models for different model animals is not uniform
In addition, AR animal models generally include two stages of sensitization and challenge, but due to different allergens, the optimal conditions for sensitization and challenge often vary greatly

Method used

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  • Allergic rhinitis animal model construction method
  • Allergic rhinitis animal model construction method
  • Allergic rhinitis animal model construction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Experimental animals: BALB / c mice, SPF grade, half male and half male, weighing 16-20 g, 5-6 weeks old, provided by Changzhou Cavens Experimental Animal Co., Ltd. The ambient temperature is 22-26°C, and the humidity is 40%-70%. 12 hours of alternating light, free access to food and water. SPF grade full-price granular mouse food was provided by Jiangsu Synergy Pharmaceutical Bioengineering Co., Ltd.

[0034] 2. Allergen: natural house dust mite allergen protein extract, item number: LG-5449, provided by LSL Corporation.

[0035] 3. Sensitization and stimulation

[0036] The times of sensitization were optimized according to the nasal symptom score of the mice after challenge and the IgE content in serum.

[0037] 3.1 Grouping of animals: 120 BALB / c mice were randomly divided into 10 groups according to body weight, with 12 mice in each group, which were negative control group, group A (2 sensitization, the excitation dose was 1 times the sensitization dose), Grou...

Embodiment 2

[0052] 1. Experimental plan

[0053] 1.1 Animal grouping: 48 BALB / c mice were randomly divided into 4 groups according to body weight, 12 in each group, respectively negative control group, low-dose natural house dust mite allergen protein extract group, middle-dose natural house dust mite The mite allergen protein extract group and the high-dose natural house dust mite allergen protein extract group were subjected to experiments after adaptive feeding for 5 days.

[0054] 1.2 Preparation of sensitization solution: Weigh the natural house dust mite allergen protein extract powder according to the dosage requirements of each group, dissolve it in PBS, and add a certain amount of Al(OH) 3 Adjuvant, and finally with PBS to make up Al(OH) 3 A sensitization solution with a content of 1% (mass percentage) and a natural house dust mite allergen protein extract concentration of 125 μg / mL, 375 μg / mL, and 1125 μg / mL.

[0055] Preparation of the excitation solution: Weigh the natural h...

Embodiment 3

[0070] 1. Experimental plan

[0071] 1.1 Grouping of animals: 20 female BALB / c mice were divided into 2 groups according to the weight-stratified random number table method, 10 in each group, respectively negative control group, dust mite allergen protein extract group, and adaptive feeding Experiments were performed 5 days later.

[0072] 1.2 Preparation of the sensitization solution: Weigh the natural D. farinae allergen protein extract powder according to the dosage requirements of each group, dissolve it in PBS, and add a certain amount of Al(OH) 3 Adjuvant, finally dilute to volume with PBS (Al(OH) 3 content of 1%), prepared into a natural D. farinae allergen protein extract concentration of 100 μg / mL sensitization solution.

[0073] Preparation of the excitation solution: Weigh the natural D. farinae allergen protein extract powder according to the dose requirements of each group, dissolve it in PBS, and finally make up the volume with PBS to prepare the natural D. far...

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Abstract

The present invention relates to an allergic rhinitis animal model, wherein a mouse allergic rhinitis (AR) animal model is established by using a dust mite allergen protein extract as an allergen, allergizing through peritoneal injection, and exciting through nose dripping. According to the present invention, the method for inducing mouse allergic rhinitis through dust mite protein is provided, and by optimizing the allergizing number, the allergizing doge, the exciting dose and the exciting mode, the mature and stable mouse AR model for treatment drug screening is prepared; compared to the traditional atomization excitation, the nose dripping excitation of the present invention has the following advantages that the allergen consumption at the excitation stage is avoided, and the waste of the allergen is avoided; and the prepared mouse AR model has advantages of low cost, simple operation and good reproducibility, and is the ideal model for studying allergic rhinitis.

Description

technical field [0001] This patent relates to the fields of new drug research and development, drug efficacy evaluation technology and experimental animal technology, in particular, it involves the construction of a mouse model of natural protein allergic rhinitis. Background technique [0002] Allergic rhinitis (Allergic Rhinitis, AR) refers to the release of mediators (mainly histamine) mediated by IgE after an atopic individual contacts an allergen, and a variety of immune active cells and Non-infectious inflammatory diseases of nasal mucosa involving cytokines and others. There are three necessary conditions for its occurrence: the specific antigen is the substance that causes the body's immune response; the atopic individual is the individual with the so-called allergic constitution; the specific antigen and the atopic individual meet. Allergic rhinitis is a global health problem that can cause symptoms such as sleep disturbance, loss of appetite, malaise, fatigue, emo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/35A61K39/39A61K49/00
CPCA61K39/35A61K49/0008A61K2039/55505
Inventor 马永江辰阳吴诚
Owner ZONHON BIOPHARMA INST
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