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Vaccine for House Dust Mite Allergen Using Naked DNA

a technology of tcell epitopes and naked dna, which is applied in the direction of antibody medical ingredients, peptide sources, drug compositions, etc., can solve the problems of poor immunogens of peptides and substantial limitations of vaccinations using peptides

Inactive Publication Date: 2009-04-23
YOO TAI JUNE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, vaccines using peptides has a substantial limitation that in the peptides are poor immunogens.

Method used

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  • Vaccine for House Dust Mite Allergen Using Naked DNA
  • Vaccine for House Dust Mite Allergen Using Naked DNA
  • Vaccine for House Dust Mite Allergen Using Naked DNA

Examples

Experimental program
Comparison scheme
Effect test

example 1

Human Epitope Vaccination

[0016]Introduction

[0017]To determine whether the vaccination with naked plasmid DNA encoding only a T-cells epitope peptide is able to suppress the allergic reaction in vivo, the mixed naked DNA plasmids encoding the five classes of human T-cell epitopes on Der p 1 and Der p 2 were used for genetic vaccination of BALB / c mice. The control mice were injected with the pcDNA 3.1 blank vector. There was a reduction in the total and Der p-specific immunoglobulin E (IgE) synthesis in the vaccinated mice compared with the control mice. In the Der p specific-IgG2a antibody response, the vaccinated mice showed more prominent responses than the control mice. Also analysis of the cytokines serum levels after immunization of Der p extract revealed that in the vaccinated mice there was an elevation in the level of interferon-γ, a Th1 cytokine associated with suppression of IgE production. The histologic studies showed that there was much less infiltration of inflammatory ...

example 2

The Effect of Vaccination with DNA Encoding Murine T-Cell Epitopes on Der p 1 and 2 Induced Immunoglobulin E Synthesis

[0054]We would like to examine the effect of vaccination with DNA encoding only the murine T-cell epitopes on the IgE production. Our results suggested that genetic vaccination indeed induced the Th1 cytokine immune responses which in turn reduced the IgE antibody production and allergic responses against Der p. Therefore it would be ideal to develop an alternative naked DNA vaccination method which could be even safer than injecting whole segments of the encoding region of Der p 1 or Der p2.

[0055]Materials and Methods

[0056]Mice

[0057]20 BALB / c mice at the age of 6-8 weeks were purchased from Jackson Laboratory (Bar Harbor, Me.) and bred at the University of Tennessee (Memphis, Tenn.). This study was performed in accordance with the PHS Policy on Humane Care and Use of Laboratory Animals, the NIH Guide for the Care and Use of Laboratory Animal Welfare Act (7 U.S.C. et...

example 3

Suppressive Effect on the Allergen-Induced Immunoglobulin E Production by the Naked DNA

[0092]We have investigated immune responses resulting from gene immunization with plasmid DNA encoding major HDM allergen (Der p 1, 2, 3, Der f 1, 2, and 3) followed by challenges with whole HDM crude extract in mice to mimic a realistic clinical setting. We have demonstrated that gene vaccination indeed induced strong Th1 immune responses, which reduced the IgB antibody production and allergic responses against HDM.

[0093]Methods

[0094]Mice

[0095]20 BALB / c mice at the age of 6-8 weeks were purchased from Jackson Laboratory (Bar Harbor, Me.) and bred at the University of Tennessee (Memphis, Tenn.) This study was performed in accordance with the PHS Policy on Humane Care and Use of Laboratory Animals, the NIH Guide for the Care and Use of Laboratory Animal Welfare Act (7 U.S.C. et seq.); the animal use protocol was approved by the Institutional Animal Care and Use Committee (IACUC) of the University o...

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Abstract

Vaccination with the DNA encoding T-cell epitopes to the house dust mite Dermatophagoides pteronyssimus (Der p) and Dermatophagoides farinae (Der f were effective in the inhibition of the allergen induced IgE synthesis. Gene therapy using T-cell epitope encoding DNA is useful in combating allergic disease.

Description

CROSS REFERENCE TO RELATED APPLICATION[0001]The present application is a Continuation application of U.S. Nonprovisional patent application Ser. No. 09 / 513,645, which claims the benefit of U.S. Provisional Patent Application Ser. No. 60 / 121,547 filed Feb. 25, 1999, which are incorporated by reference in their entirety herein.FIELD OF THE INVENTION[0002]The present invention relates to a method of vaccination using naked DNA encoding T-cell epitopes, such as those of the house dust mite Dermatophagoides pteronyssinus (Der p) and Dermatophagoides farinae (Der f), which results in the suppression of IgE production. The present invention also relates to novel combinations of mixed plasmid DNA useful in IgE suppression.BACKGROUND OF THE INVENTION[0003]Genetic vaccination with naked plasmid DNA provides a long standing cellular and humoral immune response and promotes a shift in the pattern of cytokines produced by the T-cells. Peptides derived from T-cell epitopes can downregulate cytoki...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K39/00A61K39/35A61P11/06C07K14/47
CPCA61K31/711C07K14/4713A61K2039/53A61K39/35A61P11/06A61K48/00
Inventor YOO, TAI JUNE
Owner YOO TAI JUNE
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