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Test strip for screening specific dust mite allergen IgE and preparation method of test strip

A test strip and allergen technology, which is applied in the field of test strips for specific dust mite allergen IgE screening and their preparation, can solve the problems of cumbersome operation, long test time, poor sensitivity and precision, etc. Simple operation, low cost, accurate results

Active Publication Date: 2014-08-13
BIOSINO BIO TECH & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are disadvantages of cumbersome operation, poor sensitivity and precision, and long test time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0028] Example The test strip used for IgE screening of specific dust mite allergens and its preparation method

[0029] The preparation of test strips for IgE screening of specific dust mite allergens includes the preparation of the antigen solution to be labeled, the preparation of the colloidal gold solution to be labeled, the colloidal gold-labeled cat hair antigen, the purification of the colloidal gold cat hair antigen, and the colloidal gold cat hair antigen. Size identification of gold protein particles and coating of test strips. Specific steps are as follows:

[0030] 1. Preparation of antigen solution to be labeled

[0031]Preparation of house dust mite antigen: take 50 g of house dust mite, add 200 mL of absolute ethanol to wash, then filter with cellulose acetate membrane with a pore size of 0.45 μm, collect house dust mite on the cellulose acetate membrane, wait for the ethanol to evaporate completely, pour into it Add 200mL of ether to degrease, repeat the deg...

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Abstract

The invention provides a test strip for screening specific dust mite allergen IgE. The test strip is coated with dust mite allergen labeled by colloidal gold. The colloidal gold labeling technique is utilized, the colloidal gold is used for labeling the specific dust mite allergen for the first time, then the labeled allergen is uniformly sprayed on a cellulose acetate membrane or a nitrocellulose membrane in a line manner, and the prepared test strip is used for allergen detection, the operation is simple and convenient, the result is accurate, the cost is low and other free detection can be realized.

Description

technical field [0001] The invention relates to the technical field of colloidal gold labeling, in particular to a test strip for IgE screening of specific dust mite allergens and a preparation method thereof. Background technique [0002] Allergic disease is the most common disease in contemporary society. It is caused by widespread allergens. About 5-10% of the population in my country and 5-25% of the population in the United States and Europe have suffered from allergic infestation. Among them, young children And teenagers are particularly obvious, and harm is more serious. Allergic reaction (allergy) is a pathological immune response that causes physiological dysfunction and tissue damage when the immune body is exposed to the same allergen again. Involves various clinical departments, such as internal medicine including bronchial asthma, anaphylactic shock, serum sickness, connective tissue disease, glomerulonephritis, migraine, etc., surgery including transplant rejec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/531
CPCG01N33/5308G01N33/531G01N2800/24
Inventor 蒋琳
Owner BIOSINO BIO TECH & SCI
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