46results about How to "Real-time monitoring of reaction progress" patented technology

The present invention relates to real time fluorescent PCR detecting primer and probe for food sensibiligen shrimp component, a kit and a detecting method. The real time fluorescent PCR detecting primer and probe are respectively detecting primer and probe of shrimp 16 S / r RNS gene I of which the sequence is SEQ / ID / NO. 1 to 3, detecting primer and probe of shrimp 16 S / r RNS gene II of which the sequence is SEQ / ID / NO. 4 to 6, and detecting primer and probe of arctic shrimp 16 S / r RNS gene of which the sequence is SEQ / ID / NO. 7 to 9. On the basis of this, the present invention discloses the real time fluorescent PCR detecting kit for food sensibiligen shrimp component and the corresponding detecting method. The real time fluorescent PCR detecting primer and probe for food sensibiligen shrimp component have strong specificity; the real time fluorescent PCR detecting kit and method for food sensibiligen shrimp component is simple and easy; the result is exact; and real time fluorescent PCR detecting kit and method for food sensibiligen shrimp component have high specificity and flexibility.

The invention provides a fluorescent PCR kit for qualitative detection of HLA-B*1502 gene subtypes, and belongs to the field of in-vitro nucleic acid detection. The kit comprises uracil DNA glycosylase, Taq polymerase, PCR genotyping primers and a fluorescence probe; the PCR genotyping primer sequence is as shown in SEQ ID NO: 3-4 and / or SEQ ID NO: 6-7. The kit provided in the invention has high sensitivity and good specificity, and can monitor reaction process in real time and the reaction time is short; in addition, closed tube operation is performed, and subsequent treatment is not needed, which can maximally avoid the pollution of a reaction product, thus being capable of replacing traditional cell detection.

The invention relates to a method for analyzing a result of loop-mediated isothermal amplification (LAMP), aiming at establishing a new method for analyzing the result of LAMP reaction, in which qualitative and quantitative information of a target gene is obtained by determining the change of electric conductivity of an LAMP solution; the method comprises the working steps: (1) thin wall tubes are inserted into a working sensor of an inductive conductivity gauge to serve as LAMP reaction tanks; (2) the value of electric conductivity of mixed solution before and after the biochemical reaction in every reaction tank is determined on line; and (3) the result of LAMP reaction can be deduced from the change of electric conductivity of each reaction unit so as to represent target DNA information. The method has the characteristics of being simple, convenient and fast, high in sensitivity, simple and convenient in operation, simple and inexpensive in instrument and equipment and low in expenditure, and can realize biological genetic testing at a low cost. The method can be extensively applied to the fields such as food and drug quality control, environmental monitoring, clinical diagnosis, genetically modified organism and discrimination of products thereof and the like.

The invention relates to a primer and a probe for detecting real-time fluorescence PCR of food allergen mustard components, a kit and a detection method. A sequence of an upstream primer is SEQ ID NO.1, a sequence of a downstream primer is SEQ ID NO.2, and a sequence of the probe is SEQ ID NO.3. Based on the condition, the invention discloses the real-time fluorescence PCR detection kit containing the primer and the probe sequences and the corresponding detection method. The probe and the primer have strong specificity, and the detection kit and the method have simple and easy use, accurate result and high specificity and flexibility.

The invention provides a sequencing primer for qualitative detection of TPMT genetic typing and a kit thereof, and belongs to the field of in vitro nucleic acid detection. The kit comprises uracil DNA glycosylase, Taq polymerase, a PCR reaction liquid, PCR primers, pyrosequencing primers and a reference substance. The kit provided by the invention has high sensitivity and good specificity; the PCR products can be simply treated for sequencing on a phosphate sequenator; and the kit has advantages of simple operation, short reaction time, and higher sensitivity than a gold standard-capillary electrophoresis sequencing, and is more suitable for SNP analysis.

The invention provides a sequencing primer pair for qualitatively detecting human BRAF V600E gene mutation and a kit thereof, belonging to the field of detection for in-vitro nucleic acid. The kit comprises a uracil DNA (deoxyribonucleic acid) glycosylase, a Taq polymerase, PCR (polymerase chain reaction) solution, a PCR amplification primer, a pyrosequencing primer and a positive reference substance. The kit provided by the invention is high in sensitivity, good in specificity, capable of sequencing a PCR product on a pyrosequencer after performing a simple treatment, simple and convenient in operation, short in reaction time, higher in sensitivity compared with golden standard-capillary electrophoresis sequencing, and more suitable for mutation analysis.

The invention provides a sequencing primer for qualitative detection of genetic typing of uridinediphosphoglucuronate glucuronosyltransferase 1A1 and a kit thereof, and belongs to the field of in vitro nucleic acid detection. The kit comprises uracil DNA glycosylase, Taq polymerase, a PCR reaction liquid, PCR primers, pyrosequencing primers, and a positive control product. The kit provided by the invention has high sensitivity and good specificity; the PCR products can be simply treated for sequencing on a phosphate sequenator; and the kit has advantages of simple operation, short reaction time, and higher sensitivity than a gold standard-capillary electrophoresis sequencing, and is more suitable for mutation analysis.