46results about How to "Real-time monitoring of reaction progress" patented technology

The invention relates to method for detecting the growth of macromolecule membrane induced at surface real-time and a relative equipment, which utilizes quartz crystal micro balance to detect the growth of macromolecule membrane induced at surface real-time in liquid condition. The method comprises arranging a QCM chip with inducer grown at surface into a QCM sensor chamber, feeding incomplete reaction solution to reach stable starting base line, feeding complete reaction solution and recording the frequency F detected by the QCM and the frequency change delta F of converted complete reaction solution, using ellipse polarizing equipment to measure and analyze the thickness of macromolecule membrane grown on the chip to make a curvature relative to the frequency change delta F-membrane thickness. The invention can accurately and simply control the macromolecule reaction induced at surface to control the thickness of surface modification membrane of 10<-10>m.

The invention relates to a sequencing primer pair for detecting an ALDH2 (Aldehyde Dehydrogenase 2) genotype with a pyrosequencing method and a kit thereof, and belongs to the technical field of in-vitro nucleic acid detection. The primer pair comprises a positive amplification primer, a reverse amplification primer and a sequencing primer, wherein a 5' end of the positive amplifier primer is subjected to biotin labeling. The kit comprises the positive amplification primer, PCR (Polymerase Chain Reaction) reaction liquid containing the reverse amplification primer, the sequencing primer, uracil DNA (Deoxyribose Nucleic Acid) glycosylase and Taq polymerase. The kit provided by the invention has the advantages of accurate detection result, high specificity, short detection period, easiness in operation, and capability of effectively meeting clinical examination requirements. Moreover, the kit further has the advantages that a reaction process can be monitored in real time, the reaction time is short, a PCR product can be subjected to pyrosequencing by a pyrosequencing instrument and high-flux sample detection through simple treatment, and the sensitivity is higher compared with a golden standard method, namely, a capillary electrophoresis sequencing method.

The invention discloses a real-time florescent PCR (polymerase chain reaction) detection primer of fish composition in food, a kit and a detection method. The method comprises the following steps of: designing a primer for detection of fish genes and a probe SEQ ID (sequence identification) NO.1-3 aiming at the specific gene sequence of fish; and detecting fish composition in food quantitatively by adopting a real-time florescent PCR method. The real-time florescent PCR method quantitative detection method of fish composition in researched food has great significance for improving the detection efficiency and flexibility of fish composition in food, lowering the detection cost, detecting relevant false products in the market, monitoring food safety, and improving detection technique, and has wide development prospect.

The invention provides a sequencing primer for detecting mutation of codons 12 and 13 in a second exon and a codon 61 in a third exon of a KRAS gene, and a kit thereof, and belongs to the field of in vitro nucleic acid detection. The kit comprises uracil DNA glycosylase, Taq polymerase, PCR amplification primers and sequencing primers. The kit provided by the invention has high sensitivity and good specificity; the PCR products can be simply treated for sequencing on a phosphate sequenator; and the kit has advantages of simple operation, short reaction time, and higher sensitivity than a gold standard-capillary electrophoresis sequencing, and is more suitable for mutation analysis.

The invention provides a sequencing primer for qualitative detection of TPMT genetic typing and a kit thereof, and belongs to the field of in vitro nucleic acid detection. The kit comprises uracil DNA glycosylase, Taq polymerase, a PCR reaction liquid, PCR primers, pyrosequencing primers and a reference substance. The kit provided by the invention has high sensitivity and good specificity; the PCR products can be simply treated for sequencing on a phosphate sequenator; and the kit has advantages of simple operation, short reaction time, and higher sensitivity than a gold standard-capillary electrophoresis sequencing, and is more suitable for SNP analysis.

The invention provides a sequencing primer for qualitative detection of genetic typing of uridinediphosphoglucuronate glucuronosyltransferase 1A1 and a kit thereof, and belongs to the field of in vitro nucleic acid detection. The kit comprises uracil DNA glycosylase, Taq polymerase, a PCR reaction liquid, PCR primers, pyrosequencing primers, and a positive control product. The kit provided by the invention has high sensitivity and good specificity; the PCR products can be simply treated for sequencing on a phosphate sequenator; and the kit has advantages of simple operation, short reaction time, and higher sensitivity than a gold standard-capillary electrophoresis sequencing, and is more suitable for mutation analysis.

The invention provides a sequencing primer pair for qualitatively detecting human BRAF V600E gene mutation and a kit thereof, belonging to the field of detection for in-vitro nucleic acid. The kit comprises a uracil DNA (deoxyribonucleic acid) glycosylase, a Taq polymerase, PCR (polymerase chain reaction) solution, a PCR amplification primer, a pyrosequencing primer and a positive reference substance. The kit provided by the invention is high in sensitivity, good in specificity, capable of sequencing a PCR product on a pyrosequencer after performing a simple treatment, simple and convenient in operation, short in reaction time, higher in sensitivity compared with golden standard-capillary electrophoresis sequencing, and more suitable for mutation analysis.

The invention discloses a synthetic method of glycopeptides. The method comprises the following steps: (1) an esterification reaction between Fmoc protected amino acid and an ionic liquid is carried out to obtain an ionic liquid supported unit; (2) the ionic liquid supported unit is connected with any one of monomers selected from 1) glycosylated amino acid; and 2) glycosylated amino acid and at least one Fmoc protected amino acid, and the ionic liquid is removed to obtain the glycopeptide, wherein Fmoc represents 9-fluorenylmethyloxycarbonyl. The invention has the following advantages: as the ionic liquid is a micromolecular soluble carrier with a clear structure, the reaction course can be monitored at real time by routine analysis means such as thin-layer chromatography, nuclear magnetic resonance, mass spectrum and the like; and as the ionic liquid carrier also has high load capacity, is easy to synthesize and has low cost, synthesis of glycopeptide in quantity can be realized.

The invention relates to a pyrophosphoric acid sequencing primer pair for qualitatively detecting HLA-DQ genotyping. The primer pair comprises a forward amplification primer, a reverse amplification primer and a sequencing primer, wherein the 5' terminals of the reverse amplification primer are respectively subjected to biotin labeling. The invention also relates to a pyrophosphoric acid sequencing kit for qualitatively detecting HLA-DQ genotyping. The kit comprises the amplification primers, a PCR (polymerase chain reaction) solution, the sequencing primer, a uracil DNA glycosylase and a Taq polymerase. The primer pair and kit have the advantages of accurate detection result, high specificity and short detection period, are simple to operate, and can effectively satisfy the requirements of clinical examination. Besides, the primer pair and kit can be used for monitoring the reaction process in real time, are short in reaction time, and can be used for sequencing and high-flux sample detection on a pyrophosphoric acid sequencing instrument after simply treating the PCR product. Compared with the gold standard method (capillary electrophoresis sequencing method), the primer pair and kit have the advantage of higher sensitivity.

The invention relates to a primer pair and a kit for detecting related gene polymorphism of adalimumab medication, and belongs to the technical field of in-vitro nucleic acid detection. The primer pair comprises amplification primers aiming at TNFrs1800629, KLRC1rs7301582, FCGR2Ars1801274, PTPRCrs10919563, HLA-Ers1264457, TRAF1rs3761847 and KLRD1rs2302489 allelic genes and GAPDH reference genes respectively. The kit comprises a primer solution containing the amplification primers. The kit provided by the invention is high in sensitivity which can reach one ten- thousandth, and the lowest detection limit is only 1-2copies, so that the kit is particularly suitable for detecting low-content mutation samples; and compared with a sequencing method, a detection result can be observed in real time, a product does not need gel electrophoresis detection, tube closing operation is completely carried out, and the risk of PCR product pollution is effectively reduced; and in addition, the kit has the advantages of being high in detection speed and suitable for high-throughput sample detection.

The invention relates to a primer pair and a reagent kit for detecting hepatitis B canceration susceptibility gene polymorphism, and belongs to the technical field of in vitro nucleic acid detection.The primer pair comprises a forward amplification primer, a reverse amplification primer and a sequencing primer, wherein the 5'-end of the reverse amplification primer is labeled with biotin. The kitcomprises a PCR (Polymerase Chain Reaction) solution containing a forward amplification primer, reverse amplification primer, a sequencing primer, uracil DNA (Deoxyribonucleic Acid) glycosylase, Taqpolymerase and a positive control product. The kit provided by the invention has the advantages of accurate detection result, high specificity, short detection period, and simple operation, and the clinical inspection requirements can be effectively met; in addition, the kit also has the advantages of real-time monitoring of reaction progress, and short reaction time, the PCR product can be used for sequencing on a pyrosequencing instrument after being simply treated, the sensitivity is higher than that of a gold standard method, i.e., a capillary electrophoresis sequencing method, and the kitis more applicable to mutation analysis.

The invention relates to a boron nitride quantum dot modified nano annular magnetic graphene oxide photocatalytic material and a preparation method and application thereof, the boron nitride quantum dot modified nano-ring-shaped magnetic graphene oxide photocatalytic material comprises a magnetic ferroferric oxide nano-ring, and the surface of the magnetic ferroferric oxide nano-ring is coated with graphene oxide. Boron nitride quantum dots are grafted on the graphene oxide; the preparation method comprises the following steps: preparing a nano-ring-shaped magnetic graphene oxide composite material by adopting a microwave method, and coating graphene oxide while forming a magnetic ferroferric oxide nano ring; rigid aromatic amino boron nitride quantum dots are grafted to the surface of thenano-ring-shaped magnetic graphene oxide composite material through a ring-opening reaction. The preparation process is simple and controllable, and the prepared boron nitride quantum dot modified nano-ring-shaped magnetic graphene oxide photocatalytic material is good in thermal stability and has relatively high photocatalytic degradation efficiency and dispersity; when the photocatalytic material is applied to photocatalytic degradation to remove erythromycin and roxithromycin in water, efficient and convenient solid-liquid separation can be realized.

The invention provides a solid flaky polycarboxylate superplasticizer and a method for preparing the same by a core-shell emulsion method. The superplasticizer comprises the following components in parts by weight: 18-32 parts of a special methacrylate hydrophobic monomer for emulsification, 7-13 parts of a carboxylic hydrophilic monomer for emulsification, 0.33-0.68 parts of an emulsion initiator, 0.56-0.84 parts of a chain transfer agent for emulsification, 37-62 parts of a carboxyl small monomer, 53-76 parts of an unsaturated sulfonic small monomer, 790-830 parts of a polyether macromonomer, 4-9 parts of a weak chain transfer agent, 3.6-6.8 parts of an azo initiator, 4.2-7.6 parts of an initiator, 2.3-4.2 parts of a strong chain transfer agent, and 3.4-5.6 parts of a pH regulator. The method comprises the following steps: (1) preparing an emulsifier; (2) preparing a nuclear monomer emulsion; (3) preparing a shell monomer pre-emulsion; and (4) preparing the solid flaky polycarboxylate superplasticizer. The polycarboxylate superplasticizer prepared by the core-shell emulsion method has the advantages of single molecular weight, excellent performance, strong adaptability and easiness in storage, is prepared into solid sheets, and is lower in transportation cost and suitable for long-distance transportation.