46results about How to "Real-time monitoring of reaction progress" patented technology

The invention discloses a fluorescent PCR (polymerase chain reaction) kit for quantitatively detecting human papilloma virus (HPV) 16 / 18 type infection, belonging to the field of in vitro nucleic acid diagnosis kits. The kit comprises a quantitive reference substance, a negative reference control substance, a positive reference substance, a critical positive reference substance, a fluorescent polymerase chain reaction solution, PCR serotype specific primers, a specific fluorescent probe and a DNA extraction solution. The kit comprises a multi-PCR system based on a fluorescent PCR technology, which consists of the positive and reverse primers and the fluorescent probe which aim at 16 type HPV and 18 type HPV and can simultaneously detect the DNA of the 16 type HPV and the 18 type HPV in a reaction tube under appropriate PCR conditions. The kit can simply, conveniently and rapidly detect the HPV infection in a clinical sample and has high specificity and great clinical value on early prevention and treatment of cervical carcinoma.

The invention relates to method for detecting the growth of macromolecule membrane induced at surface real-time and a relative equipment, which utilizes quartz crystal micro balance to detect the growth of macromolecule membrane induced at surface real-time in liquid condition. The method comprises arranging a QCM chip with inducer grown at surface into a QCM sensor chamber, feeding incomplete reaction solution to reach stable starting base line, feeding complete reaction solution and recording the frequency F detected by the QCM and the frequency change delta F of converted complete reaction solution, using ellipse polarizing equipment to measure and analyze the thickness of macromolecule membrane grown on the chip to make a curvature relative to the frequency change delta F-membrane thickness. The invention can accurately and simply control the macromolecule reaction induced at surface to control the thickness of surface modification membrane of 10<-10>m.

The invention relates to a sequencing primer pair for detecting an ALDH2 (Aldehyde Dehydrogenase 2) genotype with a pyrosequencing method and a kit thereof, and belongs to the technical field of in-vitro nucleic acid detection. The primer pair comprises a positive amplification primer, a reverse amplification primer and a sequencing primer, wherein a 5' end of the positive amplifier primer is subjected to biotin labeling. The kit comprises the positive amplification primer, PCR (Polymerase Chain Reaction) reaction liquid containing the reverse amplification primer, the sequencing primer, uracil DNA (Deoxyribose Nucleic Acid) glycosylase and Taq polymerase. The kit provided by the invention has the advantages of accurate detection result, high specificity, short detection period, easiness in operation, and capability of effectively meeting clinical examination requirements. Moreover, the kit further has the advantages that a reaction process can be monitored in real time, the reaction time is short, a PCR product can be subjected to pyrosequencing by a pyrosequencing instrument and high-flux sample detection through simple treatment, and the sensitivity is higher compared with a golden standard method, namely, a capillary electrophoresis sequencing method.

The invention discloses a real-time florescent PCR (polymerase chain reaction) detection primer of fish composition in food, a kit and a detection method. The method comprises the following steps of: designing a primer for detection of fish genes and a probe SEQ ID (sequence identification) NO.1-3 aiming at the specific gene sequence of fish; and detecting fish composition in food quantitatively by adopting a real-time florescent PCR method. The real-time florescent PCR method quantitative detection method of fish composition in researched food has great significance for improving the detection efficiency and flexibility of fish composition in food, lowering the detection cost, detecting relevant false products in the market, monitoring food safety, and improving detection technique, and has wide development prospect.

The invention provides a sequencing primer for detecting mutation of codons 12 and 13 in a second exon and a codon 61 in a third exon of a KRAS gene, and a kit thereof, and belongs to the field of in vitro nucleic acid detection. The kit comprises uracil DNA glycosylase, Taq polymerase, PCR amplification primers and sequencing primers. The kit provided by the invention has high sensitivity and good specificity; the PCR products can be simply treated for sequencing on a phosphate sequenator; and the kit has advantages of simple operation, short reaction time, and higher sensitivity than a gold standard-capillary electrophoresis sequencing, and is more suitable for mutation analysis.

The invention provides a sequencing primer for qualitative detection of TPMT genetic typing and a kit thereof, and belongs to the field of in vitro nucleic acid detection. The kit comprises uracil DNA glycosylase, Taq polymerase, a PCR reaction liquid, PCR primers, pyrosequencing primers and a reference substance. The kit provided by the invention has high sensitivity and good specificity; the PCR products can be simply treated for sequencing on a phosphate sequenator; and the kit has advantages of simple operation, short reaction time, and higher sensitivity than a gold standard-capillary electrophoresis sequencing, and is more suitable for SNP analysis.

The invention relates to a primer pair and kit for detecting CYP3A4 genotyping by pyrosequencing, belonging to the technical field of in vitro nucleic acid detection. The primer pair comprises a forward amplification primer, a reverse amplification primer and a sequencing primer, wherein a 5' terminal of the forward amplification primer is subjected to biotin labelling. The kit comprises the forward amplification primer, a PCR (polymerase chain reaction) liquid containing the reverse amplification primer, the sequencing primer, uracil DNA (deoxyribonucleic acid)glycosylase and Taq polymerase. The kit provided by the invention has the advantages of accurate detection results, high specificity, short detection period, simplicity in operation, capability of effectively meeting the requirements of clinical examination, capability of monitoring the reaction process in real time, short reaction time, sequencing of PCR products on a pyrosequencing instrument after the PCR products are simply treated, high throughput sample detection and higher sensitivity than gold standard methods, namely capillary electrophoresis sequencing methods.

The invention relates to a pyrosequencing primer pair for qualitatively detecting CYP2D6 genotyping; the primer pair includes a forward amplification primer and a reverse amplification primer, and a sequencing primer, and 5' ends of the forward amplification primer and reverse amplification primer are subjected to biotin labeling respectively; the invention also relates to a pyrosequencing kit for qualitatively detecting CYP2D6 genotyping; the kit includes amplification primers, PCR (polymerase chain reaction) liquid 1, PCR liquid 2, sequencing primers, uracil DNA glycosylase and Taq polymerase. The pyrosequencing primer pair and kit have the advantages that detection results are accurate, the specificity is high, detection period is short, operation is simple and clinical examination requirements can be effectively met and additionally have the advantages that reaction process can be monitored in real time, reaction time is short, PCR products can be fed to a pyrosequencing instrument for sequencing and high-throughput sample detection just through simple treatment, and the sensitivity is higher than that of a golden standard method, namely capillary electrophoresis sequencing method.

The invention provides a sequencing primer for qualitative detection of genetic typing of uridinediphosphoglucuronate glucuronosyltransferase 1A1 and a kit thereof, and belongs to the field of in vitro nucleic acid detection. The kit comprises uracil DNA glycosylase, Taq polymerase, a PCR reaction liquid, PCR primers, pyrosequencing primers, and a positive control product. The kit provided by the invention has high sensitivity and good specificity; the PCR products can be simply treated for sequencing on a phosphate sequenator; and the kit has advantages of simple operation, short reaction time, and higher sensitivity than a gold standard-capillary electrophoresis sequencing, and is more suitable for mutation analysis.

The invention discloses a real-time polymerase chain reaction (PCR) detection primer, a kit and a detection method for detecting puffer fish ingredients. The puffer fish gene detection primer and probes with the sequences of SEQ ID NO. 1-3 are designed according to the specific gene sequence of puffer fish, and the real-time fluorescent PCR method is adopted to qualitatively detect the puffer fish ingredients of food. The developed real-time fluorescent PCR qualitative detection method for detecting the puffer fish ingredients in food has importance significance in improving the detection efficiency and sensitivity of the puffer fish ingredients in food, lowering the detection cost, detecting related fake products on the market, monitoring food safety and improving a detection technology, and has wide development prospect.

The invention provides a sequencing primer pair for qualitatively detecting human BRAF V600E gene mutation and a kit thereof, belonging to the field of detection for in-vitro nucleic acid. The kit comprises a uracil DNA (deoxyribonucleic acid) glycosylase, a Taq polymerase, PCR (polymerase chain reaction) solution, a PCR amplification primer, a pyrosequencing primer and a positive reference substance. The kit provided by the invention is high in sensitivity, good in specificity, capable of sequencing a PCR product on a pyrosequencer after performing a simple treatment, simple and convenient in operation, short in reaction time, higher in sensitivity compared with golden standard-capillary electrophoresis sequencing, and more suitable for mutation analysis.

The invention relates to a primer pair and a kit for detecting folate metabolism-related gene polymorphism in hypertensive patients, and belongs to the technical field of in vitro nucleic acid detection. The primer pair comprises a forward amplification primer, a reverse amplification primer and a sequencing primer, wherein the 5' end of the reverse amplification primer is labeled with biotin. Thekit comprises a PCR (Polymerase Chain Reaction) solution containing a forward amplification primer and a reverse amplification primer, a sequencing primer, uracil DNA (Deoxyribonucleic Acid) glycosylase, Taq polymerase and a positive control product. The kit provided by the invention has the advantages of accurate detection result, high specificity, short detection period, and simple operation, and the clinical inspection requirements can be effectively met; in addition, the kit also has the advantages of real-time monitoring of reaction progress, and short reaction time, the PCR product canbe used for sequencing on a pyrosequencing instrument after being simply treated, the sensitivity is higher than that of a gold standard method, i.e., a capillary electrophoresis sequencing method, and the kit is more applicable to mutation analysis.

The invention discloses a synthetic method of glycopeptides. The method comprises the following steps: (1) an esterification reaction between Fmoc protected amino acid and an ionic liquid is carried out to obtain an ionic liquid supported unit; (2) the ionic liquid supported unit is connected with any one of monomers selected from 1) glycosylated amino acid; and 2) glycosylated amino acid and at least one Fmoc protected amino acid, and the ionic liquid is removed to obtain the glycopeptide, wherein Fmoc represents 9-fluorenylmethyloxycarbonyl. The invention has the following advantages: as the ionic liquid is a micromolecular soluble carrier with a clear structure, the reaction course can be monitored at real time by routine analysis means such as thin-layer chromatography, nuclear magnetic resonance, mass spectrum and the like; and as the ionic liquid carrier also has high load capacity, is easy to synthesize and has low cost, synthesis of glycopeptide in quantity can be realized.

The invention relates to a pyrophosphoric acid sequencing primer pair for qualitatively detecting HLA-DQ genotyping. The primer pair comprises a forward amplification primer, a reverse amplification primer and a sequencing primer, wherein the 5' terminals of the reverse amplification primer are respectively subjected to biotin labeling. The invention also relates to a pyrophosphoric acid sequencing kit for qualitatively detecting HLA-DQ genotyping. The kit comprises the amplification primers, a PCR (polymerase chain reaction) solution, the sequencing primer, a uracil DNA glycosylase and a Taq polymerase. The primer pair and kit have the advantages of accurate detection result, high specificity and short detection period, are simple to operate, and can effectively satisfy the requirements of clinical examination. Besides, the primer pair and kit can be used for monitoring the reaction process in real time, are short in reaction time, and can be used for sequencing and high-flux sample detection on a pyrophosphoric acid sequencing instrument after simply treating the PCR product. Compared with the gold standard method (capillary electrophoresis sequencing method), the primer pair and kit have the advantage of higher sensitivity.

The invention relates to a primer pair and a kit for detecting related gene polymorphism of adalimumab medication, and belongs to the technical field of in-vitro nucleic acid detection. The primer pair comprises amplification primers aiming at TNFrs1800629, KLRC1rs7301582, FCGR2Ars1801274, PTPRCrs10919563, HLA-Ers1264457, TRAF1rs3761847 and KLRD1rs2302489 allelic genes and GAPDH reference genes respectively. The kit comprises a primer solution containing the amplification primers. The kit provided by the invention is high in sensitivity which can reach one ten- thousandth, and the lowest detection limit is only 1-2copies, so that the kit is particularly suitable for detecting low-content mutation samples; and compared with a sequencing method, a detection result can be observed in real time, a product does not need gel electrophoresis detection, tube closing operation is completely carried out, and the risk of PCR product pollution is effectively reduced; and in addition, the kit has the advantages of being high in detection speed and suitable for high-throughput sample detection.

The invention discloses a kit for detecting CYP3A4*4 genetyping, belongs to the testing field of in-vitro nucleic acid, and particularly relates to a pyrophosphoric kit for detecting CYP3A4*4 genetyping. The pyrophosphoric kit for detecting CYP3A4*4 genetyping comprises uracil DNA (deoxyribonucleic acid)glycosylase, Taq polymerase, a PCR (Polymerase Chain Reaction) amplimer and a sequencing primer. The pyrophosphoric kit provided by the invention has high sensitivity and good specificity; and after simple treatment, a PCR product can be subjected to sequencing by utilizing phosphoric acid sequencing instrument, the operation is simple and convenient, the reaction time is short, the sequencing operation by adopting the pyrophosphoric kit provided by the invention has higher sensitivity when being compared with a gold-standard CE (capillary electrophoresis) sequencing operation, thus the pyrophosphoric kit is particularly suitable for mutation analysis.

The present invention relates to real time fluorescent PCR detecting primer and probe for food sensibiligen shrimp component, a kit and a detecting method. The real time fluorescent PCR detecting primer and probe are respectively detecting primer and probe of shrimp 16 S / r RNS gene I of which the sequence is SEQ / ID / NO. 1 to 3, detecting primer and probe of shrimp 16 S / r RNS gene II of which the sequence is SEQ / ID / NO. 4 to 6, and detecting primer and probe of arctic shrimp 16 S / r RNS gene of which the sequence is SEQ / ID / NO. 7 to 9. On the basis of this, the present invention discloses the real time fluorescent PCR detecting kit for food sensibiligen shrimp component and the corresponding detecting method. The real time fluorescent PCR detecting primer and probe for food sensibiligen shrimp component have strong specificity; the real time fluorescent PCR detecting kit and method for food sensibiligen shrimp component is simple and easy; the result is exact; and real time fluorescent PCR detecting kit and method for food sensibiligen shrimp component have high specificity and flexibility.

The invention relates to a pyrosequencing primer pair for qualitatively detecting ApoE genetic typing. The primer pair comprises a forward amplification primer, a reverse amplification primer and a sequencing primer, wherein the 5' end of the reverse amplification primer respectively performs biotin labeling. The invention relates to a pyrosequencing kit for qualitatively detecting the ApoE genetic typing. The kit comprises an amplification primer, PCR reaction liquid 1, PCR reaction liquid 2, a sequencing primer, uracil DNA glycosylase and Taq polymerase. The kit has the advantages of accurate detection results, high specificity, short detection period, high simplicity in operation, and capability of effectively meeting clinical examination requirements; in addition, the kit further has the advantages that the reaction process can be monitored in real time, the reaction time is short, pyrosequencing tester sequencing and high-flux sample detection can be performed by only simply treating PCR products, and the sensitivity is higher than that of a gold standard method namely a capillary electrophoresis sequencing method.

The invention relates to a primer pair and a reagent kit for detecting hepatitis B canceration susceptibility gene polymorphism, and belongs to the technical field of in vitro nucleic acid detection.The primer pair comprises a forward amplification primer, a reverse amplification primer and a sequencing primer, wherein the 5'-end of the reverse amplification primer is labeled with biotin. The kitcomprises a PCR (Polymerase Chain Reaction) solution containing a forward amplification primer, reverse amplification primer, a sequencing primer, uracil DNA (Deoxyribonucleic Acid) glycosylase, Taqpolymerase and a positive control product. The kit provided by the invention has the advantages of accurate detection result, high specificity, short detection period, and simple operation, and the clinical inspection requirements can be effectively met; in addition, the kit also has the advantages of real-time monitoring of reaction progress, and short reaction time, the PCR product can be used for sequencing on a pyrosequencing instrument after being simply treated, the sensitivity is higher than that of a gold standard method, i.e., a capillary electrophoresis sequencing method, and the kitis more applicable to mutation analysis.

The invention relates to a boron nitride quantum dot modified nano annular magnetic graphene oxide photocatalytic material and a preparation method and application thereof, the boron nitride quantum dot modified nano-ring-shaped magnetic graphene oxide photocatalytic material comprises a magnetic ferroferric oxide nano-ring, and the surface of the magnetic ferroferric oxide nano-ring is coated with graphene oxide. Boron nitride quantum dots are grafted on the graphene oxide; the preparation method comprises the following steps: preparing a nano-ring-shaped magnetic graphene oxide composite material by adopting a microwave method, and coating graphene oxide while forming a magnetic ferroferric oxide nano ring; rigid aromatic amino boron nitride quantum dots are grafted to the surface of thenano-ring-shaped magnetic graphene oxide composite material through a ring-opening reaction. The preparation process is simple and controllable, and the prepared boron nitride quantum dot modified nano-ring-shaped magnetic graphene oxide photocatalytic material is good in thermal stability and has relatively high photocatalytic degradation efficiency and dispersity; when the photocatalytic material is applied to photocatalytic degradation to remove erythromycin and roxithromycin in water, efficient and convenient solid-liquid separation can be realized.

The invention discloses a primer and a probe for detecting a source component of marten as well as a use method and an application thereof. Aiming at cytochrome oxidase b gene of marten, the method designs the primer and probe for detecting the source component of marten; and components of marten in food and feed are quantitatively detected by a real time PCR (Polymerase Chain Reaction) method. The real-time fluorescence PCR quantitative detection method for components of marten in food and feed is of significance to improve the detection efficiency and agility of the source component of marten in food, lower the detection cost, detect related bogus products in market, and monitor safety of food and improve the inspection and quarantine technology. The method has a wide development prospect.

The invention relates to a method for analyzing a result of loop-mediated isothermal amplification (LAMP), aiming at establishing a new method for analyzing the result of LAMP reaction, in which qualitative and quantitative information of a target gene is obtained by determining the change of electric conductivity of an LAMP solution; the method comprises the working steps: (1) thin wall tubes are inserted into a working sensor of an inductive conductivity gauge to serve as LAMP reaction tanks; (2) the value of electric conductivity of mixed solution before and after the biochemical reaction in every reaction tank is determined on line; and (3) the result of LAMP reaction can be deduced from the change of electric conductivity of each reaction unit so as to represent target DNA information. The method has the characteristics of being simple, convenient and fast, high in sensitivity, simple and convenient in operation, simple and inexpensive in instrument and equipment and low in expenditure, and can realize biological genetic testing at a low cost. The method can be extensively applied to the fields such as food and drug quality control, environmental monitoring, clinical diagnosis, genetically modified organism and discrimination of products thereof and the like.

The invention provides a solid flaky polycarboxylate superplasticizer and a method for preparing the same by a core-shell emulsion method. The superplasticizer comprises the following components in parts by weight: 18-32 parts of a special methacrylate hydrophobic monomer for emulsification, 7-13 parts of a carboxylic hydrophilic monomer for emulsification, 0.33-0.68 parts of an emulsion initiator, 0.56-0.84 parts of a chain transfer agent for emulsification, 37-62 parts of a carboxyl small monomer, 53-76 parts of an unsaturated sulfonic small monomer, 790-830 parts of a polyether macromonomer, 4-9 parts of a weak chain transfer agent, 3.6-6.8 parts of an azo initiator, 4.2-7.6 parts of an initiator, 2.3-4.2 parts of a strong chain transfer agent, and 3.4-5.6 parts of a pH regulator. The method comprises the following steps: (1) preparing an emulsifier; (2) preparing a nuclear monomer emulsion; (3) preparing a shell monomer pre-emulsion; and (4) preparing the solid flaky polycarboxylate superplasticizer. The polycarboxylate superplasticizer prepared by the core-shell emulsion method has the advantages of single molecular weight, excellent performance, strong adaptability and easiness in storage, is prepared into solid sheets, and is lower in transportation cost and suitable for long-distance transportation.

The invention relates to a primer and a kit for detecting human low oxygen tolerance related gene polypeptide, and belongs to the technical field of in vitro nucleic acid detection. A primer group includes a forward amplification primer, a reverse amplification primer and a sequencing primer. A 5'-end is added with a biotin label. The kit includes a forward amplification primer, PCR reaction liquid containing a reverse amplification primer, a sequencing primer, uracil DNA glycosylase, Taq polymerase and a positive control. The kit provided by the invention has the advantages of accurate detection results, high specificity, short detection period, simple operation and effectively satisfying clinical testing requirements. In addition, the kit also has the advantages of real-time monitoring on reaction progress and short reaction time, can perform direct sequencing with a pyrophosphoric acid sequencing machine through simple treatment on PCR products, has higher sensitivity than a gold standard method, namely a capillary electrophoresis sequencing method, and is more suitable for mutation analysis.

The invention provides a sequencing primer for detecting mutation of codons 12 and 13 in a second exon and a codon 61 in a third exon of a KRAS gene, and a kit thereof, and belongs to the field of in vitro nucleic acid detection. The kit comprises uracil DNA glycosylase, Taq polymerase, PCR amplification primers and sequencing primers. The kit provided by the invention has high sensitivity and good specificity; the PCR products can be simply treated for sequencing on a phosphate sequenator; and the kit has advantages of simple operation, short reaction time, and higher sensitivity than a gold standard-capillary electrophoresis sequencing, and is more suitable for mutation analysis.

The invention provides a sequencing primer pair for qualitatively detecting human BRAF V600E gene mutation and a kit thereof, belonging to the field of detection for in-vitro nucleic acid. The kit comprises a uracil DNA (deoxyribonucleic acid) glycosylase, a Taq polymerase, PCR (polymerase chain reaction) solution, a PCR amplification primer, a pyrosequencing primer and a positive reference substance. The kit provided by the invention is high in sensitivity, good in specificity, capable of sequencing a PCR product on a pyrosequencer after performing a simple treatment, simple and convenient in operation, short in reaction time, higher in sensitivity compared with golden standard-capillary electrophoresis sequencing, and more suitable for mutation analysis.

The invention provides a sequencing primer for qualitative detection of genetic typing of uridinediphosphoglucuronate glucuronosyltransferase 1A1 and a kit thereof, and belongs to the field of in vitro nucleic acid detection. The kit comprises uracil DNA glycosylase, Taq polymerase, a PCR reaction liquid, PCR primers, pyrosequencing primers, and a positive control product. The kit provided by the invention has high sensitivity and good specificity; the PCR products can be simply treated for sequencing on a phosphate sequenator; and the kit has advantages of simple operation, short reaction time, and higher sensitivity than a gold standard-capillary electrophoresis sequencing, and is more suitable for mutation analysis.