Preparation of bionic molecular imprinting electrochemical sensor and based on click chemistry and detection of food allergen

Abstract

The invention discloses preparation of a bionic molecular imprinting electrochemical sensor and based on click chemistry and a detection method of food allergen. The method includes selecting nitrine alkyl sulfhydryl fixed with the surface of an electrode to prepare a self-assembly single layer film of a nitrine end group, utilizing click chemistry reaction to conduct end group alkenyl functionalization on the self-assembly single layer film on the nitrine end group, selecting a function single body capable of reacting with the allergen to combine molecularly imprinted polymers (MIPs) of an allergen artificial antibody, utilizing the existing method to prepare a graphene material, mixing the graphene material to prepare the artificial antibody, evenly mixing template molecules of the food allergen, the function single body, the graphene, crosslinked agent, pore-foaming agent, initiator and organic solvent according to certain substance amount to prepare the bionic MIPs mingled with the graphene, connecting a prepared MIP modification work electrode to an electrochemical working station and detecting the food allergen in food extraction liquid.

Description

technical field [0001] The present invention relates to the technical field of food allergen detection, more specifically to the preparation of an electrochemical sensor capable of detecting food allergens, and the present invention also relates to a method for determining trace food allergens in a sample using the bionic molecular imprinting technology . Background technique [0002] Food allergens have become an emerging public food safety issue, especially in developed countries, surveys show that more than 1% of adults worldwide are allergic to food, and the incidence of infants and children has reached 5%- 10%. Generally speaking, food allergens are proteins or glycoproteins belonging to different families, and a small amount of food allergen proteins can cause severe allergic reactions. In recent years, the International Codex Alimentarius Commission and various developed countries have issued various food allergen labeling regulations, standards and corresponding im...

AI Technical Summary

Problems solved by technology

The most widely used method is the skin test, which can provide rapid but non-quantitative results for allergy diagnosis, and in vitro diagnosis is undoubtedly convenient and reliable for both doctors and patients, but due to ELISA test kits and Test strips have a complicated antibody preparation process and are relatively expensive; although PCR technology has development prospects, the denaturation of DNA during food processing and the maximum detection limit of this method limit its current application and development