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Instrument for detecting food allergen

A technology for detecting instruments and allergens, which is applied in the field of food allergen detection, can solve problems such as poor parallelism, long detection cycle, and low integration, and achieve the effect of improving operability

Inactive Publication Date: 2006-10-11
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complexity of food components, traditional chemical analysis cannot detect food allergens, while modern biochemical and immunological detection technologies (such as ELISA, PCR, biosensors, HPLC, etc.) It is not high, the volume of the instrument is large, the detection cycle is long, and the demand for samples is also high. The convenience and operability still need to be solved, and the parallelism is poor. It cannot measure multiple food allergens at the same time. There are still many Inadequacies

Method used

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  • Instrument for detecting food allergen
  • Instrument for detecting food allergen
  • Instrument for detecting food allergen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Extraction of food allergens with biochemical methods

[0033] Take peanuts as an example: take 1 gram of peanut samples, put them in a -70°C refrigerator for 10 minutes, take them out, cut them into pieces with scissors, and add an appropriate amount of pre-cooled ether-acetone (1:2) to degrease. Acetone degreases continuously for 48 hours, during which time the liquid is changed 5 times (every 8-10 hours), and placed under a fume hood to let it dry naturally. All the above operations were carried out on ice bath.

[0034] After degreasing, the samples were weighed and soaked in bicarbonate-saline extract (Coca’s) at a certain ratio. Add the extract at the ratio of 1g sample: 20ml Coca's solution (w / v). At the same time, the samples were fully ground and crushed with a plunger homogenizer. The crushed samples were extracted with Coca's solution for 48hr. During this period, the samples were fully stirred and placed in a refrigerator at 4°C.

[0035] Cen...

Embodiment 2

[0036] Embodiment 2: Preparation of capillary electrophoresis chip

[0037] According to attached figure 2 And attached image 3 The capillary electrophoresis chip design scheme, with glass as the chip substrate, is divided into two pieces of glass with the same size: negative film (attached figure 2 ) and cover slip (with image 3 ), the size is 60 mm x 60 mm; figure 2 Each channel is etched by laser or chemical method, the width of each channel is 20 microns, and the total number of channels is 8 with a depth of 60 microns; image 3 , open through holes at the positions marked 3, 4, 5, 6, and 7; after the bottom sheet and the cover sheet are respectively processed, the two are tightly combined with a high temperature method to make a capillary electrophoresis chip; then in the electrophoresis separation channel ( attached figure 1 In 1 and 2) a layer of polyacrylamide coating is fabricated on the tube wall, and monoclonal antibodies of different food allergens are bond...

Embodiment 3

[0038] Embodiment 3: capillary electrophoresis system

[0039] According to attached figure 1 Set up the chip capillary electrophoresis system as described in . As described in Example 1, prepare a capillary electrophoresis chip for food allergen detection, use 4 platinum wires as electrodes, and use 4 negative high-voltage power supplies with linearly controllable voltage as the electrophoresis voltage drive, and its voltage and voltage controllable range 0 to -10kV, the output power is 10kV / 5mA; the ground terminals of the four high-voltage power supplies are connected together, and two of the high-voltage terminals are used for the voltage drive of the separation channel, and are respectively connected to the electrophoresis buffer pool through the electrodes (attached figure 2 , Mark 3 of 3) and electrophoresis waste liquid tank (attached figure 2 , Mark 6 of 3), the other two high-voltage terminals are connected to the sample cell through electrodes (attached figure 2...

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Abstract

The invention discloses a tester for testing the allergen in the food, which can process qualitative and quantitative test on the allergen of food. Wherein, using buffer liquid to extract food sample to be marked by fluorescence with FITC; arranging the sample on the sample holes of capillary separate test chip; via the affinity capillary electrophoresis that combined with allergen single anti or multi anti of different foods, separating the allergen that needed to be tested; then using capillary electrophoresis to purify more; the capillary electrophoresis part uses coat hollow tube to reduce the protein adsorption of tube wall; at least, using rotary scan laser to induce the fluorescence to process multi-channel parallel test, and using photoelectric multiplier (PMT) to adsorb the fluorescence signal emitted by the sample; the data of PMT via analogue / digital convert card is stored in the control computer; and via relative software working station, the data of each channel is separated to be processed with peak identification and integral, to compare the peak parameter of sample and attain the qualitative and quantitative information of allergen component.

Description

technical field [0001] The invention relates to a method and an instrument for detecting food allergens, specifically a kind of separation and purification using affinity chip capillary electrophoresis and zonal capillary electrophoresis, and using rotary scanning laser-induced fluorescence technology to qualitatively and quantitatively detect common foods in food Allergen Methods and Apparatus. Background technique [0002] Food allergies are an important part of allergic diseases. Food allergy is an adverse reaction that people have to food, which is an allergic reaction of antibodies to foreign substances. Symptoms of food allergy include skin (urticaria, angioedema, eczema), respiratory system (bronchial asthma, rhinitis), gastrointestinal system (abdominal pain, diarrhea), and severe anaphylactic shock and death. Some foreign epidemiological surveys show that 2.5% of adults and 6%-8% of children are allergic to certain foods. The European ECRHS investigated the situa...

Claims

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Application Information

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IPC IPC(8): G01N33/561G01N33/533G01N21/64B01D57/02
Inventor 刘志刚余晓吴海强
Owner SHENZHEN UNIV
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