Method for screening detected food allergens rapidly and efficiently

An allergen and high-efficiency technology, which is applied in the field of rapid screening of allergen DNA in food, can solve the problems of narrow detection range, long detection cycle, and high reagent consumption, and achieve the effect of saving reagents

Inactive Publication Date: 2015-09-09
杭州科准检测科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This single-tube single-reaction method for detecting one or several allergen components not only has a long detection cycle, consumes a lot of reagents, but also has a narrow detection range.

Method used

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  • Method for screening detected food allergens rapidly and efficiently
  • Method for screening detected food allergens rapidly and efficiently
  • Method for screening detected food allergens rapidly and efficiently

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0031] (1) Extraction of DNA from the sample to be tested:

[0032] A. Weigh two parts of 1g of corn flour samples containing 0.1% peanut ingredients respectively, and put them into a 50mL centrifuge tube;

[0033] B. Add preheated 10mL CTAB extraction and lysis buffer (20 μL proteinase K and 20 μL RNA have been added);

[0034] C. After oscillating and suspending, incubate at 65°C for 1 hour, shake continuously during the period, and after cooling to room temperature, centrifuge at 5000g for 10 minutes;

[0035] D. Take 950 μL of the supernatant and add it to a 2 mL centrifuge tube, add an equal volume of chloroform isoamyl alcohol (24:1) and mix gently for 5 minutes, then centrifuge at 12000 rpm for 15 minutes;

[0036] E. Take 750 μL of the supernatant and add it to a 2 mL centrifuge tube, add an equal volume of chloroform isoamyl alcohol (24:1) and mix gently for 5 minutes, then centrifuge at 12000 rpm for 15 minutes;

[0037] F. Take 500 μL of the supernatant and add it...

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PUM

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Abstract

The invention discloses a method for screening detected food allergens rapidly and efficiently. Sixteen common allergen ingredients including eggs, milk, peanuts, buckwheat, cashew nuts, pistachio nuts, walnuts, carrots, filbert, almonds, wheat, soybeans, sesame, shrimps and crabs, fish and celery in life are screened and detected simultaneously according to the method. The method includes the steps of extracting DNA (deoxyribonucleic acid) from a sample, subjecting the DNA of the sample to PCR (polymerase chain reaction) amplification detection, screening and detecting the sixteen allergen ingredients simultaneously by single reaction tube through multiple channels of a PCR instrument at a time, and determining the allergen ingredients after two turns of PCR. The method has the advantages that multiple kinds of allergens are screened and detected rapidly at a time, so that a great amount of time can be saved, reagent consumption can be reduced and cost can be lowered.

Description

technical field [0001] The invention belongs to the detection technology of fast screening of allergen DNA in food, specifically refers to the rapid screening and identification of various allergen substances in food by using fluorescence quantitative PCR technology, and can determine the presence of allergies in food after the first round of reaction The original ingredients, after the second round of reaction to determine the type of allergen. Background technique [0002] In recent years, with the development of the food industry and the increase of food types, the safety of food allergens has become the focus of social attention. Food allergy is caused by IgE-mediated and non-IgE-mediated immune reactions caused by food or food additives, leading to allergic reactions in the digestive system or systemic. Allergic reactions to food allergens include different forms of clinical symptoms in the respiratory system, gastrointestinal system, central nervous system, skin, musc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6851C12Q2561/101
Inventor 宋薇张斌
Owner 杭州科准检测科技有限公司
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