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Kit for detecting IgG antibodies of allergens of 30 types of foods and preparation method

A food allergy and kit technology, applied in the field of biomedical in vitro diagnosis, to achieve the effect of improving detection efficiency, high signal value, and high stability

Inactive Publication Date: 2017-04-26
SIDERUN BEIJING MEDICAL DIAGNOSTIC PROD CO LTD
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Problems solved by technology

[0004] The purpose of the present invention is to provide a test kit and preparation method for detecting 30 food allergen IgG antibodies, thereby solving the aforementioned problems existing in the prior art

Method used

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  • Kit for detecting IgG antibodies of allergens of 30 types of foods and preparation method
  • Kit for detecting IgG antibodies of allergens of 30 types of foods and preparation method
  • Kit for detecting IgG antibodies of allergens of 30 types of foods and preparation method

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Embodiment 2

[0056] The embodiment of the present invention provides a preparation method for detecting 30 food allergen IgG antibody kits, including a method for preparing a membrane strip, and the preparation method for the membrane strip includes the following steps:

[0057] S1, using coating buffer, corn, rice, soybean, wheat, watermelon, pineapple, orange, peach, apple, mango, banana, cantaloupe, grape, strawberry, beef, chicken, egg white / yolk, pork, lamb, milk , mushrooms, tomatoes, spinach, green beans, cashew nuts, peanuts, sesame, crabs, shrimps, scallops, a total of 30 common food-specific allergens and anti-sheep IgG, human IgG diluted to the relative coating concentration to prepare the allergen coating solution Wherein, the allergen adopts lyophilized powder, and the coating buffer is dissolved in 30mmol / L tris buffer containing stabilizer, and the concentration of the allergen coating is 0.1mg / ml-1mg / ml;

[0058] S2, spot-coating the prepared allergen coating solution on ...

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Abstract

The invention discloses a kit for detecting IgG antibodies of allergens of 30 types of foods and a preparation method and relates to the technical field of biomedical in-vitro diagnosis. A linear immunoassay method is adopted, and specific IgG allergens of 30 types of common foods including corns, rice, soybeans, wheat, watermelons, pineapples, oranges, peaches, apples, mangoes, bananas, Hami melons, grapes, strawberries, beef, chicken, egg white / egg yolk, pork, mutton, milk, mushrooms, tomatoes, spinach, green soybeans, cashew nuts, peanuts, sesames, crabs, shrimps and scallops, a quality control line and a critical value line cover a membrane strip, which is prepared on a nitrocellulose membrane, in parallel; the sensitivity (a signal value) is high and the stability is high; the IgG antibodies of the allergens of 30 types of the common foods in human blood serum can be detected and tedious operation of independently detecting antibodies related to an Elisa method methodological kit one by one is overcome, so that the detection efficiency is greatly improved.

Description

technical field [0001] The invention relates to the technical field of biomedical in vitro diagnosis, in particular to a kit and a preparation method for detecting 30 food allergen IgG antibodies. Background technique [0002] At present, common food allergens in clinical laboratory samples are generally detected by enzyme-linked immunosorbent assay (Elisa). [0003] This method is to coat allergens in 96 microwell plates, incubate and react clinical samples, and finally interpret the results with a microplate reader. This method can usually detect 14 food allergen IgG at one time, and one experiment can only The detection of a single indicator has low throughput and high detection cost, which has limitations in expanding the allergen detection project. Contents of the invention [0004] The purpose of the present invention is to provide a kit and preparation method for detecting 30 food allergen IgG antibodies, thereby solving the aforementioned problems in the prior art...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/543
CPCG01N33/558G01N33/54306
Inventor 吴利娅孙冬娜张逸南李高扬刘立敏
Owner SIDERUN BEIJING MEDICAL DIAGNOSTIC PROD CO LTD
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