11 results about "Nucleic acid methods" patented technology

The present invention relates to a peptide with a length of 25 amino acids or less comprising the sequence X1-X2-X3-X4-X5-X6-X7-X8-X9-X10-X11-X12-X13 (SEQ ID No: 1) as well as to A peptide with a length of 25 amino acids or less comprising the sequence X1-X2-X3-X4-X5-X6-X7-X8-X9-X10-X11-X12-X13-X14 (SEQ ID No: 2). The present invention further relates to a nanostructure comprising a nucleic acid scaffold and at least two peptide moieties, wherein the sequence of each of the at least two peptide moieties is independently selected from the sequence of the peptide of the invention as well as pharmaceutical compositions, nucleic acids, methods and uses related thereto.

Methods for treating biological samples containing nucleic acids using high ionic strength and high temperatures. The sample can be separated into two or more phases to isolate various components in the sample, and an enzymatic reaction is performed to detect a sequence of interest. Kits for performing the methods of the invention are also provided.

Disclosed are polypeptides useful in the treatment of diseases associated with pathogenic genomic repetitive sequences, such as neurological diseases. Nucleic acid molecules and vectors encoding such polypeptides are also disclosed. Also disclosed are therapeutic uses and methods of treating such diseases; in particular, the present invention relates to therapeutic uses and methods for the treatment of Friedel's Ataxia (FRDA). Also disclosed are methods and related peptides and nucleic acids for active, long-term delivery of therapeutic molecules to target cells in vivo or in vitro.

Methods and systems provided herein address current limitations of bisulfite-based methylation sequencing by improving the quality and accuracy of nucleic acid methylation sequencing and uses thereof for detection of disease. Methods that include minimally-destructive conversion methods for methylation sequencing as well as specialized UMI adapters provide for improved quality of sequencing libraries and sequencing information. Greater accuracy and more complete methylation-state information permits higher quality feature generation for use in machine learning models and classifier generation.

Disclosed herein are methods and compositions for enhancing delivery and function of vaccine components, immunotherapy Agents, and improved delivery of nucleic acid nanostructures, nucleic acids, peptides, polypeptides, and other types of cargoes. These methods and compositions utilize design components suitable for rapid and cost-effective manufacturing, and are designed to exclusively use the process of self-assembly to form nanotherapeutics requiring no purification in many instances.

The invention relates to the technical field of kits, in particular to an oral cavity swab nucleic acid protection liquid and an extraction kit.The oral cavity swab nucleic acid protection liquid is composed of, by weight, 3.5-4.5% of anhydrous sodium acetate, 5-10% of polyethylene glycol, 5-10% of ethylenediaminetetraacetic acid and 5-10% of Tris hydrochloric acid, and the specific content is as follows: the anhydrous sodium acetate is 3.5-4.5%; 6%-8% of ethylenediamine tetraacetic acid; 0.8%-1.2% of Tris hydrochloric acid; 0.8%-1.2% of polyethylene glycol; the nucleic acid extraction method based on the paramagnetic particle method extraction kit is simple and convenient to operate, does not contain toxic reagents such as phenol chloroform and the like, can extract a plurality of samples at one time, realizes rapid diagnosis, simplifies experimental operation steps to the greatest extent, and ensures the safety of experimenters; the kit can meet the requirements of simplicity and convenience in operation, high sample flux and good nucleic acid extraction quality.

Methods and compositions for analyzing Tandem Repeats and single nucleotide polymorphisms use unlabeled, allele-specific PCR primers, a quenched fluorescent probe, and blocking nucleic acids which prevent amplification of specific numbers of tandem repeat units.

The invention provides a host nucleic acid removal method for high-throughput sequencing, and belongs to the field of pathogenic microorganism detection. By limiting the types and use concentrations of reagents, host nucleic acid substances are ensured to be fully removed. In a high host background sample, the method is suitable for tissues and blood, background host nucleic acid can be effectively reduced, and the detection rate and the detection quantity of pathogenic microorganism gene sequences are improved.

Methods, compositions, and systems for identifying autoimmune disease subject response to an immunosuppressant drug are described herein.

Methods for strand-displacement amplification of a target nucleic acid have been developed. Compositions for use according to the methods are also provided. The methods typically include steps of removing, hybridizing and extending a selectively removable or digestible primer with a strand-displacing DNA polymerase once or more than once to provide a multiplicity of copies of the target nucleic acid. Methods for strand-displacement amplification of single or double-stranded target DNA molecules are provided. In some forms, when the methods include amplification of a double-stranded nucleic acid, the methods employ one or more adapter handles that perform the function of providing a removable region on a single strand of the ds target. Compositions and kits of oligonucleotide primers and double-stranded nucleic acid adapters including selectively removable regions are also provided for use in the described methods.

Methods and kits of parts for depleting and / or enriching nucleic acid comprising the use of a rare earth metal ion.