Method of improving ginsenoside extraction rate by utilizing steam-explosion combined bio-composite enzyme technology

A biological compound enzyme and ginsenoside technology, applied in biochemical equipment and methods, enzymes, hydrolytic enzymes, etc., can solve the problem of low extraction rate of ginsenoside, achieve the effect of increasing extraction rate, reducing interference, and reducing production energy consumption

Inactive Publication Date: 2012-08-01
JILIN AGRICULTURAL UNIV
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Problems solved by technology

[0005] But in the extraction process of total ginseng saponins, the interference of ginseng cell wall is the main factor affecting the extraction rate of ginsenosides; meanwhile, in the extraction process, it will also be interfered by other macromolecular substances, such as pectin, protein, etc.; these factors make The extraction rate of ginsenosides is low

Method used

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  • Method of improving ginsenoside extraction rate by utilizing steam-explosion combined bio-composite enzyme technology

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Effect test

Embodiment 1

[0030] (1) Raw material pre-treatment: wash the raw sun-dried ginseng with water to remove impurities such as silt, and then pulverize it;

[0031] (2) Steam explosion treatment: put the pre-treated ginseng in the steam explosion tank, first feed air until the pressure in the steam explosion tank is 1.0MPa, and blast for 10 minutes; then quickly pass steam into the steam explosion tank The pressure is 1.5MPa, steam explosion treatment for 1 minute;

[0032] (3) Biological compound enzyme treatment: Suspend the raw material after steam explosion with 5 times the amount of water, add biological compound enzyme preparation (25% of cellulase, 15% of pectinase, α-starch 30% enzyme, 15% protease, 15% laccase), hydrolysis at pH 7, 50°C for 2 hours;

[0033] (4) Extraction of ginsenosides: add 15 times the amount (based on the initial raw ginseng drying parameters) of 95% ethanol to the reaction system obtained after the treatment in (3), perform ultrasonic extraction for 45 minutes,...

Embodiment 2

[0036] (1) Pre-treatment of raw materials: wash the aboveground part of American ginseng to remove impurities such as sediment and then pulverize it;

[0037] (2) Steam explosion treatment: put the pre-treated raw materials in the steam explosion tank, first feed air until the pressure in the steam explosion tank is 0.8MPa, and blast for 15 minutes; then quickly pass steam into the steam explosion tank The pressure is 1.5MPa, steam explosion treatment for 2 minutes;

[0038](3) Biological compound enzyme treatment: Suspend the raw material after steam explosion with 10 times the amount of water, add biological compound enzyme preparation (30% of cellulase, 10% of pectinase, α-starch 35% enzyme, 10% protease, 15% laccase), hydrolysis at pH 7, 50°C for 2 hours;

[0039] (4) Extraction of ginsenosides: add 10 times the amount of water (based on the amount of the aerial part of American ginseng) to the reaction system obtained in (3), heat and reflux for extraction for 1 hour, fi...

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Abstract

The invention provides a steam-explosion combined bio-composite enzyme preparation, and a method for producing ginsenoside by using ginseng, American ginseng, ginseng stems and leaves, American ginseng stems and leaves and the like as raw materials. Compared with a conventional method, the method has the characteristics of high yield of ginseoside extract, high content of extract saponin, high extraction rate of gnsenoside and the like.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine modernization, and relates to a steam-explosion-combined biological composite enzyme preparation, which uses ginseng, American ginseng, ginseng stems and leaves, American ginseng stems and leaves, etc. as raw materials to produce ginsenosides. Compared with the conventional method, the method has the characteristics of high yield of ginsenoside extract, high content of saponin in the extract, high extraction rate of ginsenoside and the like. Background technique [0002] Ginseng (Panax ginseng C.A. Meyer) and American ginseng (Panax quinquefolium Linn) are Araliaceae plants. As a traditional medicinal material, it has a history of thousands of years and is widely used in countries all over the world. Ginseng greatly invigorates the vital energy, restores the veins and strengthens the spleen, nourishes the spleen and lungs, promotes fluid production, and calms the nerves. American gins...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/20C12N9/00C12N9/42C12N9/26C12N9/50C12N9/02
Inventor 赵岩张连学郜玉钢吴鹏王立岩何忠梅金达明翟为
Owner JILIN AGRICULTURAL UNIV
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