Composite enzyme prepn for degrading agricultural waste effectively and its prepn process

A technology for compound enzyme preparation and agricultural waste is applied in the field of high-efficiency degradation of agricultural waste compound enzyme preparation and its preparation, which can solve the problems of low fermentation temperature, insufficient fermentation, incomplete killing of harmful organisms, etc. The effect of biogas production, energy saving, and promotion of digestion and absorption rate

Inactive Publication Date: 2007-10-03
唐东升 +1
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Problems solved by technology

Because the temperature of the fermentation heap is not high, the fermentat

Method used

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  • Composite enzyme prepn for degrading agricultural waste effectively and its prepn process
  • Composite enzyme prepn for degrading agricultural waste effectively and its prepn process
  • Composite enzyme prepn for degrading agricultural waste effectively and its prepn process

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Embodiment

[0013] A compound enzyme preparation for efficient degradation of agricultural waste, which includes the following enzyme species and activities: xylanase, whose enzyme activity is ≥100,000 U / g; β-glucanase, whose activity is ≥80,000 U / g ; Cellulase, whose enzyme activity is ≥28,000 U / g; acid protease, whose enzyme activity is ≥56,000 U / g; lipase, whose enzyme activity is ≥15,000 U / g pectinase, whose enzyme activity is ≥0.6 million U / g; α-amylase, whose enzyme activity is ≥12,000 U / g.

[0014] The preparation method of the high-efficiency degradation compound enzyme preparation of the above-mentioned agricultural waste, the method comprises the following process steps:

[0015] 1. Strain preparation:

[0016] Xylanase, glucanase, cellulase, acid protease, lipase, pectinase, α-amylase, etc. are all used in vitro directed evolution of enzyme molecules to produce high-yield enzyme live strains.

[0017] Using the theory of molecular breeding, using the directional screening tec...

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Abstract

The present invention relates to enzyme preparation, and is especially one kind of composite enzyme preparation for degrading agricultural waste effectively and its preparation process. The composite enzyme preparation includes xylanase with enzyme activity not lower than 100 KU/g, beta-dextranase with enzyme activity not lower than 80 KU/g, cellulose with enzyme activity not lower than 28 KU/g, acid protease with enzyme activity not lower than 56 KU/g, lipase with enzyme activity not lower than 15 KU/g, pectase with enzyme activity not lower than 6 KU/g, and alpha-amylase with enzyme activity not lower than 12 KU/g. Its preparation process includes the steps of spawn preparation, enzyme preparation production, compounding, etc. The present invention may be used in feed production, methane production and other fields.

Description

technical field [0001] The invention relates to an enzyme preparation and a preparation method thereof, in particular to a compound enzyme preparation for efficiently degrading agricultural waste and a preparation method thereof. Background technique [0002] my country is a large agricultural and populous country, with a large amount of agricultural waste and kitchen leftovers. At present, most of the organic waste in rural areas of our country is directly discharged, piled up or applied to farmland without treatment, and a small part adopts the method of traditional composting and natural fermentation. . However, the initial number of fermentation microbial flora of natural compost is small, the proliferation is slow, and it is often not resistant to high temperature, so the compost fermentation temperature rises slowly and the fermentation cycle is long, usually more than 2 months. Because the temperature of the fermentation heap is not high, the fermentation is not suffi...

Claims

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Application Information

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IPC IPC(8): C12N9/98C12N9/24C12N9/46C12N9/42C12N9/50C12N9/28A23K1/165C02F11/04C05F9/04C05F17/00A23K20/189
CPCY02B30/52Y02B30/123Y02A40/20Y02P20/129
Inventor 唐东升魏起星
Owner 唐东升
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