Production method of heat resisting xylanase and use in feed

Abstract

The invention discloses a production method of a heat-resistant xylanase that utilizes a thermophilic fungus DSM10635 and the usage of the heat-resistant xylanase in feedstuff. As a feedstuff addition agent, the heat-resistant xylanase can be directly used or mixed with other enzymes so as to prepare a composite enzyme agent. The heat-resistant xylanase production method has following advantages: a mixture of dry corncob powder and flour water is used as a culture medium, thus being very simple and cheap; processing of raw materials is simple; product extraction is simple, product can be obtained by crashing materials after solid fermentation, and the whole process does not have waste discharge; the culture medium contains little water and consequently has reduced chances of bacteria contamination.

Description

technical field [0001] The present invention relates to the field of biochemical enzymes, in particular to a production method of a heat-resistant xylanase and the use of the heat-resistant xylanase in feed. Background technique [0002] Xylan is a polyfive-carbon sugar whose main chain is connected by multiple xylopyranosyl groups through 1,4-xylosidic bonds. A variety of short substituents of different sizes are attached to the side chain, and are connected to the arabinose residue through the O2 and O3 atoms on the xylose residue, so it is also called arabinoxylan. Sometimes arabinoxylan molecules also contain a small amount of other side chain residues, such as six-carbon sugar, glucuronic acid, ferulic acid. Arabinoxylan is covalently bound to other components of the cell wall through these residues. The number and distribution of arabinose side chains vary with different types and varieties of grains, and the ratio of arabinose to xylose is about 0.65-0.74:1. The su...

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Problems solved by technology

At the same time, it reduces the diffusion rate of nutrients in the intestinal chyme in the intestinal tract, hindering nutrient absorption

[0009] 2. Affect the activity of endogenous enzymes in the digestive tract and stimulate the compensatory increase of digestive organs

[0011] 3. Affect the digestion and absorption of fat

[0013] 4. Promoting the proliferation of harmful microorganisms in the intestine, affecting animal health

For poultry, wet feces are easy to stick around the cloaca, contaminate poultry and poultry eggs, and provide a place for microbial fermentation, resulting in a large amount of ammonia, and can promote the reproduction of fungal spores, which is not good for the health of poultry

[0015] 5. Physical barrier effect, affecting the digestion of nutrients

At present, the main ingredient in the feed is corn, and amylopectin is difficult to use, resulting in waste of feed

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