Production method of heat resisting xylanase and use in feed

A technology of xylanase and production method, applied in the field of biochemical enzymes, can solve problems such as stimulating compensatory increase of digestive organs, polluting poultry and poultry eggs, and affecting animal health

A technology of xylanase and production method, applied in the field of biochemical enzymes, can solve problems such as stimulating compensatory increase of digestive organs, polluting poultry and poultry eggs, and affecting animal health

CN101487000AInactive Publication Date: 2009-07-22熊海容
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  • Production method of heat resisting xylanase and use in feed
  • Production method of heat resisting xylanase and use in feed
  • Production method of heat resisting xylanase and use in feed

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0064] The production flow chart of thermostable xylanase of the present invention is as figure 1 Shown:

[0065] Production of the thermostable xylanase of the invention using the thermophilic fungus DSM10635

[0066] 1. Substrate selection

[0067] Such as figure 2 As shown, the thermophilic fungus DSM10635 was studied at 50°C with cheap corn husks, corncobs, corn stalks, bran, broad leaves (poplar) and needles (pine) as raw materials, and the enzyme production was obtained The order of size is: corn bran medium (1673.54IU / g) > corn cob medium (1306.34IU / g) > corn stalk medium (379.07IU / g) > bran medium (155.09IU / g) > broad Leaf wood medium (19.11IU / g) > coniferous wood medium (2.03IU / g).

[0068] The results indicated that corn husks and corn cobs were the best inductive substrates for the production of thermostable xylanase at 50°C by the thermophilic fungus DSM10635, and their application in the industrial production of enzymes would greatly reduce production costs ...

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Abstract

The invention discloses a production method of a heat-resistant xylanase that utilizes a thermophilic fungus DSM10635 and the usage of the heat-resistant xylanase in feedstuff. As a feedstuff addition agent, the heat-resistant xylanase can be directly used or mixed with other enzymes so as to prepare a composite enzyme agent. The heat-resistant xylanase production method has following advantages: a mixture of dry corncob powder and flour water is used as a culture medium, thus being very simple and cheap; processing of raw materials is simple; product extraction is simple, product can be obtained by crashing materials after solid fermentation, and the whole process does not have waste discharge; the culture medium contains little water and consequently has reduced chances of bacteria contamination.

Description

technical field [0001] The present invention relates to the field of biochemical enzymes, in particular to a production method of a heat-resistant xylanase and the use of the heat-resistant xylanase in feed. Background technique [0002] Xylan is a polyfive-carbon sugar whose main chain is connected by multiple xylopyranosyl groups through 1,4-xylosidic bonds. A variety of short substituents of different sizes are attached to the side chain, and are connected to the arabinose residue through the O2 and O3 atoms on the xylose residue, so it is also called arabinoxylan. Sometimes arabinoxylan molecules also contain a small amount of other side chain residues, such as six-carbon sugar, glucuronic acid, ferulic acid. Arabinoxylan is covalently bound to other components of the cell wall through these residues. The number and distribution of arabinose side chains vary with different types and varieties of grains, and the ratio of arabinose to xylose is about 0.65-0.74:1. The su...

Claims

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Application Information

Patent Timeline
22 Jul 2009
Publication
CN101487000A
IPC
C12N9/24; A23K1/165; C12R1/645
Inventors
熊海容