Crosslinked compositions comprising collagen and demineralized bone matrix, methods of making and methods of use

Inactive Publication Date: 2005-01-27
WARSAW ORTHOPEDIC INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0005] According to a first aspect of the invention, a composition is provided comprising demineralized bone matrix (DBM) and a collagen protein wherein the composition is crosslinked. The composition can be chemically crosslinked with a carbodiimide. The carbodiimide can be N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC). The composition can be chemically cross-linked with a carbodiimide in the presence of N-hydroxysuccinimi

Problems solved by technology

While the compositions provided by this approach provided a stable structure for inv

Method used

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  • Crosslinked compositions comprising collagen and demineralized bone matrix, methods of making and methods of use
  • Crosslinked compositions comprising collagen and demineralized bone matrix, methods of making and methods of use
  • Crosslinked compositions comprising collagen and demineralized bone matrix, methods of making and methods of use

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Embodiment Construction

[0011] According to one embodiment of the invention, a composition comprising DBM in a collagen carrier is provided which provides an osteoconductive matrix for cell migration and which has an extended duration after implantation in a patient. According to further embodiment of the invention, a chemical crosslinking method is provided to crosslink a composition comprising collagen and DBM. During crosslinking, collagen molecules can be crosslinked together through reactive groups present on the collagen molecules. Also during crosslinking, collagen molecules can be crosslinked to the DBM due to the presence of reactive surface groups on the DBM. As a result, an osteoconductive matrix that lasts longer after implantation and that can still be turned over in vivo as bone is formed is provided. This method also allows control of the amount of DBM added to the matrix and optimization of the material handling characteristics of the resulting composition.

[0012] According to a further emb...

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Abstract

A composition comprising a collagen protein and demineralized bone matrix is described wherein the composition is chemically cross-linked with a carbodiimide such as N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC). The crosslinking reaction can be conducted in the presence of N-hydroxysuccinimide (NHS). The collagen can be in a porous matrix or scaffolding. The DBM can be in the form of particles dispersed in the collagen. A method of making the composition is also described wherein a collagen slurry is cast into the desired shape, freeze dried to form a porous scaffolding and infitrated with a solution comprising the cross-linking agent. The composition can be used as an implant for tissue (e.g., soft tissue or bone) engineering.

Description

BACKGROUND Technical Field [0001] The present application relates generally to bioprosthetic devices and, in particular, to chemically cross-linked collagen based carriers comprising demineralized bone matrix (DBM) and to the use of these materials as implants such as, for example, osteoinductive implants. Background of the Technology [0002] Various materials have been used to repair or regenerate bone or soft tissue that has been lost due to either trauma or disease. Typically, implantable bone repair materials provided a porous matrix (i.e., scaffolding) for the migration, proliferation and subsequent differentiation of cells responsible for osteogenesis. While the compositions provided by this approach provided a stable structure for invasive bone growth they did not promote bone cell proliferation or bone regeneration. [0003] Subsequent approaches have used bone repair matrices containing bioactive proteins which when implanted into the bone defect provided not only a scaffoldin...

Claims

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Application Information

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IPC IPC(8): A61L24/00A61L24/10A61L27/22A61L27/36
CPCA61L24/0005A61L24/102A61L27/227A61L2430/02A61L27/3645A61L27/3687A61L27/3608
Inventor DRAPEAU, SUSAN J.TORRIANNI, MARKEVERAERTS, FRANKMCKAY, WILLIAM F.
Owner WARSAW ORTHOPEDIC INC
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