Crosslinked compositions comprising collagen and demineralized bone matrix, methods of making and methods of use

Abstract

A composition comprising a collagen protein and demineralized bone matrix is described wherein the composition is chemically cross-linked with a carbodiimide such as N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC). The crosslinking reaction can be conducted in the presence of N-hydroxysuccinimide (NHS). The collagen can be in a porous matrix or scaffolding. The DBM can be in the form of particles dispersed in the collagen. A method of making the composition is also described wherein a collagen slurry is cast into the desired shape, freeze dried to form a porous scaffolding and infitrated with a solution comprising the cross-linking agent. The composition can be used as an implant for tissue (e.g., soft tissue or bone) engineering.

Description

BACKGROUND Technical Field [0001] The present application relates generally to bioprosthetic devices and, in particular, to chemically cross-linked collagen based carriers comprising demineralized bone matrix (DBM) and to the use of these materials as implants such as, for example, osteoinductive implants. Background of the Technology [0002] Various materials have been used to repair or regenerate bone or soft tissue that has been lost due to either trauma or disease. Typically, implantable bone repair materials provided a porous matrix (i.e., scaffolding) for the migration, proliferation and subsequent differentiation of cells responsible for osteogenesis. While the compositions provided by this approach provided a stable structure for invasive bone growth they did not promote bone cell proliferation or bone regeneration. [0003] Subsequent approaches have used bone repair matrices containing bioactive proteins which when implanted into the bone defect provided not only a scaffoldin...

AI Technical Summary

Benefits of technology

[0005] According to a first aspect of the invention, a composition is provided comprising demineralized bone matrix (DBM) and a collagen protein wherein the composition is crosslinked. The composition can be chemically crosslinked with a carbodiimide. The carbodiimide can be N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC). The composition can be chemically cross-linked with a carbodiimide in the presence of N-hydroxysuccinimi

Problems solved by technology

While the compositions provided by this approach provided a stable structure for invasive bone growth they did not promote bone cell proliferation or bone regeneration.

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