Preparation method of lysozyme molecular imprinting-quantum dot nanoscale fluorescent probe

A nano-fluorescent probe and molecular imprinting technology, applied in the field of preparation of quantum dot fluorescent probes, can solve the problems of poor stability, high price of natural antibodies, cumbersome extraction process, etc., and achieve large specific surface area and easy selective recognition of template molecules. , the effect of excellent performance

Active Publication Date: 2012-01-11
NANKAI UNIV
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Problems solved by technology

However, due to the high price, cumbersome extraction process and poor stability of natural antibodies, the development of artificial antibody technology (molecular imprinting technology) is one of the ways to solve this problem

Method used

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  • Preparation method of lysozyme molecular imprinting-quantum dot nanoscale fluorescent probe
  • Preparation method of lysozyme molecular imprinting-quantum dot nanoscale fluorescent probe
  • Preparation method of lysozyme molecular imprinting-quantum dot nanoscale fluorescent probe

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Embodiment

[0025] A preparation method of lysozyme molecularly imprinted-quantum dot nanometer fluorescent probe, which uses cadmium telluride quantum dots as the carrier, and lysozyme (N-acetylmuramohydrolase) as the template molecule (the lysozyme used in this patent Enzymes are purchased from Sigma-Aldrich), combined with the selectivity of molecular imprinting technology and the fluorescent properties of quantum dots to synthesize nano-fluorescent probes, including the following steps:

[0026] 1) Mix 0.064g tellurium powder (Te), 0.36g NaBH 4 Mix with 8mL of distilled water, carry out the reduction reaction under the conditions of nitrogen protection and stirring to prepare NaHTe solution, and quickly add the prepared NaHTe solution to the CdCl solution of mercaptopropionic acid (MPA) 2 solution, the CdCl 2 The preparation materials of the solution are 12.5mL cadmium chloride, 53 μL mercaptopropionic acid and 180mL distilled water, then the pH of the solution is adjusted to 9.0 wit...

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Abstract

The invention discloses a preparation method of a lysozyme molecular imprinting-quantum dot nanoscale fluorescent probe. The preparation method adopts quantum dots as carriers and lysozyme as a template molecule and modifies a molecular imprinting polymer layer on surfaces of the quantum dots to synthesize the novel lysozyme molecular imprinting-quantum dot nanoscale fluorescent probe through combining selectivity of a molecular imprinting technology and fluorescent characteristics of the quantum dots. Specially, the preparation method comprises the following steps of preparing quantum dots, modifying surfaces of the quantum dots by denatured bovine serum albumin, forming a molecular imprinting layer through a polymerization reaction initiated by a selected functional monomer and cross-linking reagents, and washing away the template molecule by selected eluent. The lysozyme molecular imprinting-quantum dot nanoscale fluorescent probe can combine accuracy and specific identification characteristics of molecular imprinting and high sensitivity characteristics of quantum dot fluorescence detection and has high selectivity and sensitivity of template molecule identification. The preparation method has simple processes, good reproducibility between different batches, good application prospects in selective identification and detection of lysozyme in an actual sample.

Description

technical field [0001] The invention relates to a preparation technology of a quantum dot fluorescent probe, in particular to a preparation method of a lysozyme molecular imprint-quantum dot nanometer fluorescent probe. Background technique [0002] Quantum dots (quantum dots, QD) are semiconductor nanocrystals composed of II-VI or III-V elements that can receive excitation light to generate fluorescence. Quantum dots have excellent fluorescence characteristics such as wide and continuous excitation spectrum, narrow and symmetrical emission spectrum, adjustable emission wavelength, high fluorescence quantum yield, and good photochemical stability. In recent years, the application of quantum dots (semiconductor nanoparticles) in the fields of biology and medicine has greatly expanded the depth and breadth of quantum dot research, and has become one of the most viable development directions in the fields of biology and medicine. Moreover, quantum dots play a huge role in biol...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C09K11/88C09K11/02
Inventor 李文友张卫何锡文张玉奎
Owner NANKAI UNIV
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