Method for producing D-alpha-hydroxybutyric acid
A technology of hydroxybutyric acid and sodium hydroxybutyrate, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low enantiomeric excess ratio, and achieve simple separation, easy access, and bottom The effect of high concentration
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0055] (1) Preparation of complete microbial cells containing NAD-independent hydroxyacid dehydrogenase: Pseudomonas putida ATCC12633 was selected for routine culture, and NAD-independent hydroxyacid dehydrogenase was detected by high-performance liquid chromatography (HPLC). Hydrogenase activity, until its activity reaches 140 units / liter, stop the fermentation culture; separate and collect the thalline, wash the thalline twice with pH 7.4 potassium phosphate buffer, suspend the thalline resuspension in deionized water, make the thalline concentration reach 200 grams of wet cells / liter to obtain a complete cell suspension containing NAD-independent hydroxyacid dehydrogenase, and store at 4°C for later use;
[0056] (2) Heat denaturation to inactivate NAD-independent D-hydroxyacid dehydrogenase: take the complete cell suspension containing NAD-independent hydroxyacid dehydrogenase prepared in step (1) and place it in a 60°C water bath for treatment 10 minutes, store at 4°C for...
Embodiment 2
[0064] (1) Preparation of complete microbial cells containing NAD-independent hydroxyacid dehydrogenase: Pseudomonas aeruginosa (Pseudomonas aeruginosa) ATCC 10145 was selected and cultured routinely. Dependent on the activity of hydroxyacid dehydrogenase, when the activity reaches 160 units / liter, stop the fermentation culture; separate and collect the bacteria, wash the bacteria twice with pH 7.4 potassium phosphate buffer, and resuspend the bacteria in deionized water. Make the bacterial cell concentration reach 200 g wet cells / liter to obtain a complete cell suspension containing NAD-independent hydroxyacid dehydrogenase, and store it at 4°C for later use;
[0065] (2) Heat denaturation to inactivate NAD-independent D-hydroxyacid dehydrogenase: take the complete cell suspension containing NAD-independent hydroxyacid dehydrogenase prepared in step (1) and place it in a water bath at 55°C for treatment 15 minutes, store at 4°C for later use;
[0066] (3) Resolution of racem...
Embodiment 3
[0073] (1) Preparation of crude enzyme solution containing NAD-independent L-hydroxyacid dehydrogenase: Pseudomonas stutzeri (Pseudomonasstutzeri) ATCC 17588 was selected for routine culture, and NAD was detected by high-performance liquid chromatography (HPLC) during the period Independent L-hydroxyacid dehydrogenase activity, when the activity reaches 170 units / liter, stop the fermentation culture; separate and collect the bacteria, wash the bacteria twice with pH 7.4 potassium phosphate buffer, resuspend the bacteria in In deionized water, make the cell concentration reach 200 g wet cells / liter to obtain a complete cell suspension containing NAD-independent L-hydroxyacid dehydrogenase, crush the complete cell suspension with ultrasonic waves to obtain a crude enzyme solution, store at 4°C spare;
[0074] (2) Heat denaturation to inactivate NAD-independent D-hydroxyacid dehydrogenase: take the crude enzyme solution containing NAD-independent hydroxyacid dehydrogenase prepare...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com