The invention provides a method for preparing (S)-1-(3,5-bis (
trifluoromethyl)) phenylethanol by
microbial transformation. The (S)-1-(3,5-bis (
trifluoromethyl)) phenylethanol is prepared according to The invention provides a method for preparing (S)-1-(3,5-bis (
trifluoromethyl)) phenylethanol by
microbial transformation. The (S)-1-(3,5-bis (trifluoromethyl)) phenylethanol is prepared according tothe following steps: 1-(3,5-bis (trifluoromethyl)) phenylethanol functioning as substrate is catalyzed by
fermentation products of
Candida tropicalis 104 functioning as
enzyme source so as to be reducthe following steps: 1-(3,5-bis (trifluoromethyl)) phenylethanol functioning as substrate is catalyzed by
fermentation products of
Candida tropicalis 104 functioning as
enzyme source so as to be reduced asymmetrically at 28-34 DEG C, and transformation solution is separated and purified after the asymmetric reduction reaction. The
Candida tropicalis 104 is chosen to be used as
enzyme-producing stred asymmetrically at 28-34 DEG C, and transformation solution is separated and purified after the asymmetric reduction reaction. The Candida tropicalis 104 is chosen to be used as enzyme-producing strain after optimum strain seeking, and 1-(3,5-bis (trifluoromethyl)) phenylethanol is catalyzed to be reduced asymmetrically in a single-phase aqueous
system so as to generate (S)-1-(3,5-bis (trifluoroain after optimum strain seeking, and 1-(3,5-bis (trifluoromethyl)) phenylethanol is catalyzed to be reduced asymmetrically in a single-phase aqueous
system so as to generate (S)-1-(3,5-bis (trifluoromethyl)) phenylethanol which has an
enantiomeric excess value of over 99 percent and can be well applied.methyl)) phenylethanol which has an
enantiomeric excess value of over 99 percent and can be well applied.