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Bionic mineralized collagen gel and preparation method and application thereof

A collagen gel and biomimetic mineralization technology, applied in the field of biomimetic materials, can solve the problems of uneven repair effect, inability to achieve nano-scale bone tissue biomimetic, etc., and achieve the effect of high-efficiency biomimetic mineralization

Active Publication Date: 2021-04-09
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In recent years, the development of tissue engineering has brought new hope for bone regeneration and repair, but the repair effect is uneven
The basic unit of bone is calcium phosphate mineralized collagen with an ordered nanoscale structure, but most of the current calcium phosphate materials are large-scale materials with tens or even hundreds of nanometers, which cannot realize nanoscale bone tissue bionics

Method used

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  • Bionic mineralized collagen gel and preparation method and application thereof
  • Bionic mineralized collagen gel and preparation method and application thereof
  • Bionic mineralized collagen gel and preparation method and application thereof

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preparation example Construction

[0031] The invention provides a preparation method of bionic mineralized collagen gel, comprising the following steps:

[0032] (1) Type I collagen acid solution is formed into collagen gel;

[0033] (2) Cross-linking the collagen gel to obtain a cross-linked collagen gel;

[0034] (3) calcium salt solution, polyaspartic acid solution and phosphate solution are mixed to obtain mineralization solution;

[0035] (4) immersing the cross-linked collagen gel in a mineralization solution for mineralization to obtain the biomimetic mineralized collagen gel.

[0036] In the present invention, the type I collagen acid solution is formed into a collagen gel.

[0037] In the present invention, there is no special limitation on the source of the type I collagen acid solution, and a method well known to those skilled in the art can be used. In a specific embodiment of the present invention, the type I collagen acid solution is preferably prepared by dissolving the type I collagen acid s...

Embodiment 1

[0061] Extraction of Type I Collagen: Sampling: Take 50g of pig’s hoof tendon, soak it in double-antibody water for 15 minutes, cut it into minced meat, dissolve it in 500mL Tris buffer, and leave it overnight at 4°C. Filter through gauze, wash with distilled water for 5 times, dilute to 1.5L, add 45mL of acetic acid, 5g of pepsin, and stir at 4°C for 3 days. Centrifugation and filtration: Centrifuge at 4000 rpm for 25 minutes at 4°C to recover collagen, dilute with 0.5M acetic acid, and filter again with gauze. Salting out: 0.9M sodium chloride solution salting out, stirring overnight at 4°C. Centrifugation: 4°C, 4000rpm, centrifuge for 25 minutes, dissolve in 0.5M acetic acid, overnight at 4°C. Dialysis: Pack the collagen into dialysis bags, place in 2L deionized water, stir at 4°C for 3 days, and change the water every 8 hours during this period. Freeze-drying: Divide the collagen in the dialysis bag, overnight at -80°C, transfer to a freeze dryer, and dry for 3 days to o...

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Abstract

The invention provides bionic mineralized collagen gel and a preparation method and application thereof, and belongs to the technical field of bionic materials. Polyaspartic acid is adopted as a stabilizer, a mineralization solution containing calcium ions and phosphate ions can form amorphous calcium phosphate nano-clusters, after collagen hydrogel is mixed with the amorphous calcium phosphate nano-clusters, the amorphous calcium phosphate nano-clusters can enter collagen fibrils in the collagen hydrogel and be combined with free carboxyl, then nucleating and phase change are performed to form hydroxyapatite, the hydroxyapatite grows in order in the long axis direction of the collagen fibrils, and mineralization in the collagen fibrils in the collagen gel is achieved.

Description

technical field [0001] The invention relates to the technical field of biomimetic materials, in particular to a biomimetic mineralized collagen gel and its preparation method and application. Background technique [0002] As the hard tissue in the locomotor system, bone provides a strong lever for muscle contraction to achieve motor function. Bone tissue regeneration will be abnormal when patients are accompanied by severe trauma, body disorders, osteoporosis, primary or metastatic bone tumors, insufficient blood supply, or infection. At the same time, there are about 8.9 million new patients with abnormal bone union every year in the world, and this number continues to rise with the progress of population aging. At present, the gold standard for improving bone regeneration ability is autologous bone grafting and allograft bone grafting, but the available resources are limited, and there are complications such as pain and paresthesia at the donor site. [0003] In recent y...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/50A61L27/12A61L27/02A61L27/18A61L27/52
CPCA61L27/24A61L27/50A61L27/12A61L27/025A61L27/18A61L27/52A61L2430/02A61L2400/12C08L75/02
Inventor 茵梓陈晓雷庭筠张涛赵艳艳
Owner ZHEJIANG UNIV
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