102results about How to "Shorten hemostasis time" patented technology

The invention discloses a method for preparing a modified regenerated cellulose / alginate hemostatic composite material, and relates to a method for preparing a hemostatic material, belonging to the technical field of biomedical composite materials. The invention aims to solve the technical problem that an existing alginate material has an unsatisfactory hemostatic effect, poor structure integrity, and relatively low mechanical strength and stability. The method comprises a first step of modification of regenerated cellulose; a second step of preparation of an oxidized regenerated cellulose / sodium alginate water solution; a third step of molding of the hemostatic composite material; and a fourth step of cross-linking and solidification of the hemostatic composite material. According to the modified regenerated cellulose / alginate hemostatic composite material, due to the introduction of a sodium carboxylic acid structure at the C6 site of the regenerated cellulose molecule through the selective oxidization of a TEMPO-NaClO-NaBr oxidation system, the hemostatic property, structure integrity, mechanical strength and stability of the modified regenerated cellulose / alginate hemostatic composite material are improved, and the defects of poor wet strength, low mechanical integrity and easy deformation of ordinary alginate non-woven fabrics are overcome.

The present invention relates to compositions comprising complexes of human cells and polymer fibers and methods of their use for therapeutic purposes. Methods of making such compositions are also provided. The present invention encompasses compositions comprising poly-β-1→4-N-acetylglucosamine polymers and stored platelets and their use for promoting wound healing and achieving hemostasis.

The invention relates to a medical flocking hemostasis material for stopping bleeding and protecting arteries, veins and wounds of tissues, application of the medical flocking hemostasis material and a method for preparing the medical flocking hemostasis material. The medical flocking hemostasis material comprises a flocking layer and a base layer, the flocking layer is used as a wound contacting layer and made of functional fibers, and the functional fibers are fixed on the upper surface of the base layer in a flocking method. The flocking method comprises a mechanical flocking method and an electrostatic flocking method. The medical flocking hemostasis material can be used for stopping bleeding and protecting the arteries, the veins and the wounds of the tissues, and further can be used as a medicine slow-release carrier. The invention further discloses method for controlling bleeding of the arteries, bleeding of the veins and bleeding of the wounds of the tissues by the aid of the medical flocking hemostasis material. Compared with a hemostasis material in the prior art, the medical flocking hemostasis material has the advantages that a hemostasis performance, comfortableness and the like are greatly improved.

The present invention relates to compositions comprising complexes of human cells and polymer fibers and methods of their use for therapeutic purposes. Methods of making such compositions are also provided. The presen t invention encompasses compositions comprising poly-.beta.-1.fwdarw.4-N-acetylglucosamine polymers and stored platelets and their use for promoting wound healing and achieving hemostasis.

The invention discloses a novel chitosan hemostatic sponge and a preparation method thereof. The preparation method comprises the following steps of: stirring 5-20 percent of water-soluble chitosan carboxylate or carboxymethyl chitosan and 0.1-5 percent of 407 or 188 poloxamer in water at a low speed to fully dissolving state; adding 0-10 percent of glycerol, stirring at a high speed, foaming for 30 minutes to one hour, and performing injection molding; rapidly refrigerating at the temperature 40 DEG C below zero; demolding; refrigerating and drying till the water content is below 3 percent; and cutting, sterilizing and packing to obtain the novel chitosan hemostatic sponge.

The invention discloses a preparation method for efficient anti-bacterial composite hemostatic sponge. The preparation method comprises the following steps: S1, adding sodium chloride and phosphonium salt into a sodium alginate solution to obtain a solution A; S2, adding an emulsifier into dispersion liquid to obtain a solution B, and preparing a 4 to 6 percent calcium chloride solution to serve as a solution C; S3, adding the solution A into the solution B in a water-oil ratio of 1 to (3-7) by an emulsion-crosslinking method, stirring to form an emulsion, adding the solution C into the emulsion for reacting, curing, centrifuging, washing and drying to obtain porous alginate drug-carrying microspheres; S4, dissolving chitosan hydrochloride into water to obtain an aqueous solution, adding the porous alginate drug-carrying microspheres prepared in the step S2 and a hemostatic medicament, performing freeze drying repeatedly, washing and sterilizing to obtain the hemostatic sponge. The hemostatic sponge has high water absorptivity, can rapidly stop bleeding, plug wounds, inhibit wound infection and effectively promote healing of the wounds; the thrombus risk is avoided.

An apparatus for sealing a puncture in a blood vessel is disclosed having a tissue-confining device for compressing tissue in the vicinity of the puncture, which longitudinally extends between a proximal end and a distal end and is connected to an adjustable artery clamp for controllably applying pressure, such as by a fluid circuit, onto the blood vessel, so as to reduce the hemostatis time. The artery clamp comprises in a preferred embodiment a proximal plunger and a distal plunger positioned upstream and downstream, respectively, to the puncture. The apparatus is also suitable for treating a pseudoaneurysm.

The invention discloses collagen-based composite hemostasis powder and a preparation method thereof. The method is characterized by comprising the following steps: modifying type I collagen by using dencichine, preparing the modified type I collagen into a solution with the concentration of 1-2 percent by using polylysine-modified chitosan; preparing medical gelatin into a 1-3% solution; preparing the modified chitosan into a 1-3% solution; blending the three solutions according to a mass ratio of 0.5-1:2-8:8-2; stirring at the temperature of 4-10 DEG C for 8-10 hours, and performing freeze drying to prepare composite sponge; sequentially impregnating the composite sponge in a modifier and a blood coagulation factor in an ultrasonic cleaner, fully washing, performing freeze drying, and grinding by a grinder to prepare the collagen-based composite hemostasis powder. The powder has the effects of rapidly and effectively stopping bleeding, resisting bacteria, diminishing inflammation, relieving pain, promoting wound healing and repairing, has high adhesion property, hydrophilicity, biological degradability and biocompatibility and is low in price and suitable for wound hemostasis or repair.

The invention relates to a water-soluble oxidized regenerated cellulose hemostatic material and a preparation method thereof, solving the problem that the traditional oxidized regenerated cellulose materials are water-insoluble and have the defects of high polymerization degree, poor biological absorbability and slow haemostatic speed. The water-soluble oxidized regenerated cellulose hemostatic material is prepared from an alcoholic solution of oxidized regenerated cellulose and strong alkali. The preparation method comprises the following steps of: (1) neutralizing and modifying; (2) washing; and (3) drying. The product provided by the invention can be used in the medical hemostasis field.

The invention discloses a preparation method and applications of carboxylation chitosan. The preparation method comprises following steps: a dimethylsulfoxide solution of glutaric anhydride is delivered into a sealed reactor, and is heated, chitosan is added for reaction, an obtained product is reacted in sodium hydroxide solution so as to obtain carboxylation chitosan; carboxylation chitosan is dissolved in a solid acid solution, calcium chloride is added for dissolving, an obtained mixture is subjected to spray drying so as to obtain carboxylation chitosan hemostatic particles; carboxylation chitosan is dissolved in the solid acid solution, calcium chloride is added for dissolving, the obtained mixture is subjected to freeze drying so as to obtain carboxylation chitosan hemostatic sponge; carboxylation chitosan is dissolved in an acid solution, an obtained mixed solution is injected into a mould, the mould is sealedly coated with a one-way permeable membrane, and is immersed in an alkaline medium so as to obtain chitosan gel, the obtained chitosan gel is washed with deionized water until pH value reaches 7, and is immersed in a calcium chloride solution for crosslinking, an obtained product is collected, washed, immersed in a glycerine solution, collected, and drained, is added into absolute ethyl alcohol for single-surface steaming, and is dried so as to obtain carboxylation chitosan hemostatic membranes.

The invention discloses a graphene oxide grafted modified oxidized regenerated cellulose hemostatic material and a preparation method thereof, relating to a hemostatic material and a preparation method thereof. The graphene oxide grafted modified oxidized regenerated cellulose hemostatic material aims to solve the problems that a modified material of the existing oxidized regenerated cellulose is poor in mechanical strength and biological absorption of the material and small in improvement degree of hemostatic performance. The graphene oxide grafted modified oxidized regenerated cellulose hemostatic material is prepared from graphene oxide containing amino groups on the surface and oxidized regenerated cellulose. The preparation method comprises the following steps: I, preparing graphene oxide subjected to acylating chlorination; II, preparing graphene oxide containing the amino groups; and III, performing chemical grafting to obtain the graphene oxide grafted modified oxidized regenerated cellulose hemostatic material. The graphene oxide grafted modified oxidized regenerated cellulose hemostatic material disclosed by the invention can be used for reducing the bleeding-stopping time by 7-17% when the material is used for stopping bleeding. The graphene oxide grafted modified oxidized regenerated cellulose hemostatic material and the preparation method thereof can be obtained according to the invention.

The invention provides a blood vessel closure structure which can be completely degraded. The structure especially aims at arterial puncture mouth closure. The structure comprises an anchor in a bloodvessel lumen, an anti-hemorrhagic compression object outside the lumen and a connecting suture; the portions, kept in the body, of the structure are all made from material which can be completely degraded; according to one particularly preferable embodiment, the anchor in the blood vessel lumen is closely attached to the blood vessel inner wall and anchors a puncture mouth; the anti-hemorrhagic compression object presses the blood vessel outer wall tightly and blocks the puncture mouth; the suture connects the anchor and the compression object, and through an auto-tightened knot, the sandwichstructure is fixed. By means of the blood vessel closure structure which can be completely degraded, the burden of longstanding manual pressing for hemostasis of a doctor is reduced, and time in bedof a patient is shortened; compared with a blood vessel suture closure structure, the blood vessel blocking closure structure has the advantages that the operative steps are simplified, and the learning cost and the hemostasis failure rate of a surgeon are reduced; the objects implanted in the body can be completely degraded, so that a suture removing process brought by use of non-degradable material is avoided.

The invention provides a chitosan fiber-based efficient hemostatic material and a preparation method thereof. The preparation method comprises the following steps of spinning chitosan to obtain a chitosan fiber base material, and grafting carboxyl and catechol structures on the chitosan fiber base material at the same time. The hemostatic material can quickly absorb moisture in blood to swell, so that the hemostatic rate is remarkably improved. The carboxyl is introduced, so that the hemostatic material has super-strong imbibition capacity, platelets in blood of a bleeding part are quickly adhered and aggregated to form thrombus, hydroxyl on the catechol group can be complexed with iron ions in red blood cells, the red blood cells are aggregated to play a role in stabilizing blood clots, and finally, the effect of quickly stopping bleeding is achieved. The efficient hemostatic material provided by the invention is especially suitable for the condition of massive bleeding.

The invention relates to an antibacterial and hemostatic microballoon containing berberine and preparation and application of the antibacterial and hemostatic microballoon. Hemostatic can be used in abody and can be rapidly degraded and absorbed. A compound antibacterial and hemostatic material with carboxymethyl chitosan, sodium alga acid, collagen and the berberine as raw materials is adopted,the characteristics of antibacteria and adhesivity of the berberine are used, and the material is enabled to obtain the antibacterial performance while the hemostatic effect is further enhanced. Meanwhile, under the environment of simulated body liquid after hemostasis, antibacterial and hemostatic effective componenets such as the carboxymethyl chitosan, the sodium alga acid, the collagen and theberberine are degraded, the characteristics of related materials such as polysaccharides and polypeptide capable of being hydrolyzed and micromolecule being easily metabolic and decomposed are used,and thus, the effect that the antibacterial and hemostatic material can be rapidly degraded and absorbed in the body is achieved.

The invention relates to a preparation method for a porous zeolite-containing hemostatic gel dressing based on human-like collagen and zeolite. According to the weight ratio (1:10) to (1:20) of zeolite to human-like collagen, an appropriate amount of zeolite is added into LHC solution to obtain a mixture, inorganic salt, zinc beta-diiminate complex as a compound cross-linking agent and 1,2,7,8-diepoxyoctane are added into the mixture and uniformly stirred, the pH value of the solution is regulated to the acidic condition, the solution is put into 40 DEGC-80 DEG C water bath and kept for 0.5 to 5 hours, high-temperature vapor treatment and washing are then carried out twice to remove salt and cross-linking agent residues, and after drying and Co-60 sterilization, the zeolite-containing hemostatic gel dressing is obtained. The material has good air permeability, adhesion resistance and a quick hemostatic effect, moreover, the gel medium disperses the zeolite particles, so that the problem of heat release of the zeolite in the process of exerting the hemostatic effect is greatly mitigated, and compared with gel without zeolite, the porous zeolite-containing hemostatic gel dressing remarkably shortens the hemostatic time to 15 to 25 seconds on average.

The invention provides an administration method, which includes the following steps that: a fluid is introduced into a container for holding a medicament, so that the fluid flows along a pressure relief channel, and discharged at a pressure relief through hole of the container, after the flow velocity of the fluid is stabilized, during use, the pressure relief through hole is closed and the fluidenters into a pressurized channel, flows along the pressurized channel, is discharged from the outlet of the pressurized channel and the medicament is pushed into the outlet of the container. The method provided by the invention can deliver the medicament in the container in a more stable and consistently balanced manner and in a more gradual manner, effectively avoiding the medicament being spread on the mirrored field due to the counteraction of target tissue caused by the sudden release of pressure, making administration (such as hemostatic agent) more precise, improving the continuity of the laparoscopic operation, improving the hemostatic effect, and shortening the operation time.

The invention discloses a growth factor compound dressing capable of adhering a tissue fissure and a preparation method thereof. The compound dressing comprises an alginate gel layer and a non-woven fabric layer, wherein the alginate gel layer is prepared from sodium alginate, calcium carbonate, glucolactone, glycerinum, sodium carboxymethyl cellulose, allantoin, alpha-cyanoacrylate, cell growth factors and a protective agent. The dressing can be used for replacing a suture line to a certain extent, radically effectively promoting wound healing to prevent pains of surgical suturing for patients with relatively deep wounds, and effectively reducing generation of scars.

The invention discloses a polysaccharide hemostatic dressing as well as a preparation method and an application thereof. The polysaccharide hemostatic dressing is membrane or sponge-like product prepared by carrying out a cross-linking reaction on aminopolysaccharides and oxidized polysaccharides, wherein the aminopolysaccharides refer to one or more of hyaluronic acid, chondroitin sulfate, heparin, chitosan, hydroxyethyl chitosan, hydroxypropyl chitosan, sulfonated chitosan, hydroxybutyl chitosan, carboxymethyl chitosan, succinyl chitosan or other chitosan derivatives; the oxidized polysaccharides refer to one or more of oxidized polysaccharides containing dialdehyde groups; and saccharide units containing the dialdehyde groups account for 1-70% of total saccharide units of the polysaccharide molecule. The polysaccharide hemostatic dressing disclosed by the invention has an obvious hemostasis effect, is capable of obviously shortening the hemostasis time of the wound surface, is non-toxic, and has excellent biocompatibility. The polysaccharide hemostatic dressing can be used for preparing medical apparatuses and instruments for traumatic hemostasis and surgical hemostasis, servesas hemostatic dressing medical apparatuses and instruments to be developed, and has wide market prospects.

The invention discloses a nanogold / lysine / oxidized regenerated cellulose composite hemostatic material and a preparation method thereof, relates to a hemostatic material and a preparation method thereof, and aims at solving the problems of obstruction to the biological absorption of a hemostatic material and slight improvement of the hemostatic performance due to a fact that the skin of a mammal is irritant to other body cells at a contact position and a large whitish granulation lump is formed at a use position in the use process of an existing oxidized regenerated cellulose modified material serving as the hemostatic material. The nanogold / lysine / oxidized regenerated cellulose composite hemostatic material is prepared from the nanogold and the oxidized regenerated cellulose on the surface of which the lysine is grafted; the preparation method of the hemostatic material comprises the steps of firstly, chemically grafting the lysine to the oxidized regenerated cellulose, secondly, preparing the aqueous dispersion of the nanogold, and 3, carrying out self-assembling of the nanogold, thereby obtaining the nanogold / lysine / oxidized regenerated cellulose composite hemostatic material. The invention successfully provides the nanogold / lysine / oxidized regenerated cellulose composite hemostatic material and the preparation method thereof.

The invention provides a nanogel hemostatic material modified with modified glucan as well as preparation and an application of the nanogel hemostatic material. The nanogel hemostatic material is formed by polymerizing modified glucose and monomers containing vinyl, wherein the modified glucose is glucose modified with glycidyl methacrylate. The nanogel hemostatic material has excellent water absorption and good biocompatibility, the size is increased after the nanogel hemostatic material absorbs water to expand, a polymer hydrogel layer is formed and can block a wound to stop bleeding, modified glucan nonogel has an excellent hemostatic effect on traumatic hemorrhage such as venous hemorrhage, arterial hemorrhage, body surface hemorrhage as well as internal organ hemorrhage such as liver hemorrhage, is a good and novel hemostatic and has good application prospects in clinical trauma. The invention further provides a preparation method of the modified polysaccharide modified nanogel hemostatic material. The raw materials are cheap and easy to obtain, the reaction condition is mild, the reaction operation is simple, and post-treatment is convenient.

The invention belongs to the technical field of biomedicine materials, and specifically relates to a modified chitosan-alginate-dragon's blood composite nano hemostatic material having a porous microsphere structure. Modified chitosan and alginate are compounded through an electrostatic effect to prepare a wall material of the microsphere; and dragon's blood is taken as the core material. The corematerial is embedded in the wall material. The mass ratio of the wall material to the core material is 1-3:1-5. The modified chitosan is amphiphilic modified chitosan. The alginate is one of sodium alginate and magnesium alginate. The invention also provides a preparation method of the composite nano hemostatic material. The provided modified chitosan-alginate-dragon's blood composite nano hemostatic material can rapidly stop bleeding of wounds, is also capable of continuously releasing drugs, inhibiting wound infection, and promoting wound healing, has good biological compatibility, and doesnot have any side or toxic effect. The problem that dragon's blood is difficult to dissolve during the preparation process is solved. The encapsulation rate of dragon's blood is increased, and the action time of drugs is prolonged.

The invention provides a water-soluble hemostatic material and a preparation method thereof and relates to a hemostatic material and a preparation method thereof, wherein the water-soluble hemostatic material and the preparation method thereof can be used for overcoming the problems that the existing oxidized regenerated cellulose materials are not water-soluble, have poor biological absorbability and slow hemostatic speed. The water-soluble hemostatic material is prepared from oxidized regenerated cellulose carboxylic sodium fabrics and hydrochloric acid. The preparation method comprises the following steps: winding the oxidized regenerated cellulose carboxylic sodium fabrics on a glass porous core of an acidification rotary reactor; adding the hydrochloric acid for close acidification; decompressing, filtering and washing with absolute ethanol; and freezing and drying to obtain the water-soluble hemostatic material. The water-soluble hemostatic material can be used as a hemostatic material.

The invention aims to provide fiber suitable for clinical application, in particular to the application of hemostasis, scalding, chronic wound treatment and others. The invention is a hemostatic material, wherein the hemostatic material includes fiber, and the material is possessed of biodegradability; the hemostatic material is characterized in that the fiber includes a polymer expressed by the following structural formula: FORMULA, wherein R is at least one of groups consisting of -NH2, -CH2CH20H, -OCH2CH2CH2OH, -OH, -ONa, -OK, -OCa; m, n and p respectively represent the quantity percent of corresponding repeat unit in the polymer molecule and can meet the following relationship formula: m+n+p=1, p / (m+n+p)=0.05-0.30; the degree of substitution D is defined as m / (m+n+p), the total degree of substitution of fiber is defined as Ds, and the degree of substitution of polymer at the center of the fiber cross section is defined as D0; when the degree of substitution of the edge of the fiber cross section is defined as D, the following relationship formula can be satisfied: Ds=0.09-0.8, Do / D=0-0.7.

The invention belongs to the technical field of medical materials, and particularly relates to alkyl chitosan-graphene oxide composite sponge and a preparation method and application thereof. The composite sponge comprises alkyl chitosan and graphene oxide adsorbed on alkyl chitosan, and adsorption capacity of graphene oxide is 3-28%. The composite sponge obtained by taking alkyl chitosan as a matrix and compositing graphene oxide and alkyl chitosan has excellent hemostatic performance and blood absorbing capacity. Embodiment results show that when the composite sponge is used for hemostasis,in-vitro whole blood coagulation time is less than 58s, hemostasis time of a rabbit femoral artery bleeding model is less than 155s, bleeding amount is less than 5.4g, and hemostasis effect is betterthan pure alkyl chitosan sponge or graphene oxide powder.

The present invention provides an absorbable semi-flowing cross-linked polypeptide biological surgical bleeding-stopping substance, which comprises a gelatin and a cross-linking agent, wherein a cross-linking agent is added to a gelatin solution to make high-reactivity amino acid side chains react with each other so as to form a cross-linking structure, and iron-calcium ions are added to increase the cross-linking degree so as to finally form the three-dimensional network cross-linked macromolecular polypeptide. According to the present invention, the absorbable semi-flowing cross-linked polypeptide biological surgical bleeding-stopping substance can be safely absorbed by human tissues, has characteristics of semi-fluidity, viscosity and adhesion, can promote the coagulation process of human body through physical and chemical principles, can rapidly stop bleeding, can treat surgical bleeding in the case of no damage on tissues so as to improve the convenience and the flexibility of surgical hemostasis and improve the safety and the effectiveness of surgical hemostasis, and can achieve the satisfactory bleeding-stopping effect in the cases of multi-point bleeding, large-area errhysis, bleeding in the narrow lacuna, hidden bleeding point or difficultly distinguished bleeding point, and bleeding difficultly operated and controlled by the existing bleeding-stopping methods.

The invention discloses a preparation method of an RGD-M13 phage / polylysine / oxidized regenerated cellulose composite haemostatic material, and relates to a preparation method of a haemostatic material. The invention aims at solving the problems of an existing oxidized regenerated cellulose modified haemostatic material that a haemostatic time is lightly improved, and the mechanical strength and bio-absorbing property of the oxidized regenerated cellulose are decreased. The method comprises the following steps: I. self-assembly of polylysine; II. self-assembly of an RGD-M13 phage; and III. cleaning and drying, so that the RGD-M13 phage / polylysine / oxidized regenerated cellulose composite haemostatic material is obtained. The RGD-M13 phage / polylysine / oxidized regenerated cellulose composite haemostatic material prepared by the invention, when applied to haemostasis, can shorten the haemostatic time by 25.17%-41.95%. The invention provides the RGD-M13 phage / polylysine / oxidized regenerated cellulose composite haemostatic material.

The invention discloses a keratin nanoparticle and a preparation method thereof. The average particle size of the keratin nanoparticle ranges from 105 nm to 150 nm. The keratin nanoparticle is prepared in the following steps that firstly, a dispersing solution is prepared, wherein a dilute acetic acid solution with the pH being 2-3 or a diluted hydrochloric acid solution with the pH being 1-3 is prepared for use; secondly, a keratin aqueous solution with the mass concentration being 0.5-1.25% is prepared, wherein deionized water is added into a keratin extract, stirred and dissolved; thirdly, a nano suspension is prepared, wherein the keratin aqueous solution is added into the prepared dispersing solution of a preset amount in a dropwise mode, and the dispersing solution is subjected to ultrasonic dispersing while dropwise addition is conducted to obtain a keratin nano suspension; fourthly, the suspension obtained in the third step is subjected to high-speed centrifugation, after centrifugation, centrifugal supernate is poured, and powder obtained after centrifugal settlement and drying blocks and is ground to obtain the keratin nanoparticle. The method is easy to implement, and the prepared keratin nanoparticle is uniform in appearance, can be used for medicine carriers or hemostatic materials and has good in-vitro coagulation and in-vivo hemostasis effects.

The invention discloses biologic hemostatic powder and a preparation method thereof, which belongs to the field of biological medicine. Cassava starch is used as a raw material to form a loose porousstructure, coagulation of blood platelets can be accelerated, the blood coagulation can be promoted, and the hemostatic time can be shortened; gamma-polyglutamic acid and calcium chloride are used asraw materials to be chelated with calcium ions to prepare chelate additive gamma-polyglutamic acid on a bleeding site, and the existence of the calcium ions on a wound can further accelerate the bloodcoagulation; collagen is mixed a cross-linking agent genipin to combine heparin sodium and collagen to obtain an epidermal growth promotion additive, the healing and cell activation rebuilding of a bleeding wound can be promoted, and the restoration function is excellent; and chitosan and dodecyl aldehyde are added to generate Schiff base, so that the probability of mesoporous additive remainingin blood can be reduced, the safety performance can be ensured, and the irritation action for the wound can be reduced. The problems that the existing hemostatic powder is poor in hemostatic effect and low in hemostatic speed can be solved.