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Preparation method of RGD-M13 phage/polylysine/oxidized regenerated cellulose composite haemostatic material

A RGD-M13, regenerated cellulose technology, applied in pharmaceutical formulations, pharmaceutical science, bandages, etc., can solve the problems of reduced mechanical strength and bioabsorbable properties of oxidized regenerated cellulose, and a small increase in hemostasis time, so as to promote wound healing. Healing, improvement of hemostatic properties, reduction of hemostasis time

Active Publication Date: 2016-04-13
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to solve the problem that the hemostatic time of the existing hemostatic material modified by oxidized regenerated cellulose is small, and the mechanical strength and bioabsorbability of oxidized regenerated cellulose are reduced, and a RGD-M13 bacteriophage is provided Preparation method of polylysine / oxidized regenerated cellulose composite hemostatic material

Method used

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specific Embodiment approach 1

[0020] Embodiment 1: This embodiment is a preparation method of RGD-M13 bacteriophage / polylysine / oxidized regenerated cellulose composite hemostatic material, which is completed according to the following steps:

[0021] 1. Self-assembly of polylysine: immerse the oxidized regenerated cellulose into an aqueous solution of polylysine with a mass fraction of 0.01% to 0.1%, then let it stand at room temperature for 10min to 30min, and then oxidize the regenerated cellulose from The mass fraction is 0.01%~0.1% polylysine aqueous solution is taken out, obtains the oxidized regenerated cellulose modified by polylysine; Use deionized water to wash the oxidized regenerated cellulose modified by polylysine 10 times~ 20 times, and then vacuum freeze-drying at a temperature of -70°C to -40°C for 40h to 80h to obtain dry polylysine-modified oxidized regenerated cellulose;

[0022] The mass fraction of carboxyl groups in the oxidized regenerated cellulose described in step 1 is 16% to 24%;...

specific Embodiment approach 2

[0033] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the molecular weight of polylysine in the polylysine aqueous solution with a mass fraction of 0.01% to 0.1% in step 1 is 150,000 to 300,000. Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0034] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the oxidized regenerated cellulose in step 1 exists in the form of short filaments, filaments or fabrics. Other steps are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses a preparation method of an RGD-M13 phage / polylysine / oxidized regenerated cellulose composite haemostatic material, and relates to a preparation method of a haemostatic material. The invention aims at solving the problems of an existing oxidized regenerated cellulose modified haemostatic material that a haemostatic time is lightly improved, and the mechanical strength and bio-absorbing property of the oxidized regenerated cellulose are decreased. The method comprises the following steps: I. self-assembly of polylysine; II. self-assembly of an RGD-M13 phage; and III. cleaning and drying, so that the RGD-M13 phage / polylysine / oxidized regenerated cellulose composite haemostatic material is obtained. The RGD-M13 phage / polylysine / oxidized regenerated cellulose composite haemostatic material prepared by the invention, when applied to haemostasis, can shorten the haemostatic time by 25.17%-41.95%. The invention provides the RGD-M13 phage / polylysine / oxidized regenerated cellulose composite haemostatic material.

Description

technical field [0001] The invention relates to a preparation method of a hemostatic material. Background technique [0002] Various accidents inevitably occur in daily life. During emergency treatment and operation, medical accidents or even death may be caused by massive bleeding. Therefore, local and effective rapid hemostasis of patients is very important, and effective control of bleeding and reducing bleeding time have become important measures to reduce the mortality of patients. Commonly used clinical hemostatic materials such as hemostatic gauze, hemostatic fiber, and hemostatic bandage have limitations in use, such as longer hemostasis time, easy adhesion to the wound and difficult dressing change, and powerlessness to wound infection and suppuration. Rapid hemostasis and functional hemostasis will be the development direction of hemostatic drugs in the future. [0003] Commonly used absorbable hemostatic materials include fibrin glue, gelatin sponge, oxidized ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/28A61L15/20A61L15/36A61L15/42
CPCA61L15/20A61L15/28A61L15/36A61L15/42A61L2400/04
Inventor 吴亚东黄玉东毛传斌王芳
Owner HARBIN INST OF TECH
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