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Preparation method and applications of carboxylation chitosan

A technology of carboxylated chitosan and carboxylated chitosan gel, which is used in medical science, absorbent pads, bandages, etc., can solve the problems of not finding chitosan hemostatic products, unsatisfactory bleeding effect, and weakened hemostatic effect. , to achieve the effects of good wound adhesion, strong antibacterial, and shortened hemostasis time.

Inactive Publication Date: 2014-06-11
江春平 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention provides a kind of preparation method of novel carboxylated chitosan, purpose is in order to solve following technical problem: 1, existing chitosan dressing coagulation time is long, 2. Poor water absorption and poor adhesion to wounds. 3. Some antibacterial effects, but not strong

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0048] A kind of preparation method of novel carboxylated chitosan

[0049] (1) Add glutaric anhydride in dimethyl sulfoxide solution (3g glutaric anhydride dissolved in 40ml dimethyl sulfoxide) into a 250 ml airtight flask, and heat to 60°C;

[0050] (2) Put 6g of chitosan with a degree of deacetylation of 55% and a molecular weight of 20,000 into a closed flask, and stir the reaction for 30 minutes with the rotor to carboxylate the chitosan;

[0051] (3) After the reaction, take out the chitosan, put it into 0.1mol / L NaOH solution at 25°C for 1 hour, and then wash it with ethanol for 6 times.

[0052] The carboxylated chitosan described in this embodiment has a short hemostatic time, can be applied to wounds with a large amount of bleeding, and has a good fit with the skin surface, and its hemostatic time for the rabbit dorsal auricular artery bleeding model is 23 ± 6s , the bleeding time applied to the rabbit popliteal artery bleeding model is 81 ± 17s, and the blood abs...

Embodiment 2

[0054] A kind of preparation method of novel carboxylated chitosan

[0055] (1) Add glutaric anhydride in dimethyl sulfoxide solution (3g glutaric anhydride dissolved in 40ml dimethyl sulfoxide) into a 250 ml airtight flask, and heat to 60°C;

[0056] (2) Put 6g of chitosan with a degree of deacetylation of 99% and a molecular weight of 500,000 into a closed flask, and stir the reaction for 30 minutes with the rotor to carboxylate the chitosan;

[0057] (3) After the reaction, take out the chitosan, put it into 0.1mol / L NaOH solution at 25°C for 1 hour, and then wash it with ethanol for 6 times.

[0058] The carboxylated chitosan described in this embodiment has a short hemostatic time, can be applied to wounds with a large amount of bleeding, and has a good fit with the skin surface, and its hemostatic time for the rabbit dorsal auricular artery bleeding model is 22 ± 5s , the bleeding time applied to the rabbit popliteal artery bleeding model is 79 ± 12s, and the blood ab...

Embodiment 3

[0060] A kind of preparation method of using carboxylated chitosan to prepare carboxylated chitosan hemostatic granules

[0061] Dissolve 1g of carboxylated chitosan into 100ml of 1% (m / v) citric acid solution, then dissolve into 0.2g of calcium chloride, and then spray dry with a conventional spray dryer to obtain carboxylated chitosan hemostatic particles .

[0062] The carboxylated chitosan hemostatic particles described in this embodiment have a short hemostatic time, can be applied to wounds with a large amount of bleeding, and have a good fit with the skin surface. The hemostatic time of the rabbit dorsal auricular artery bleeding model is 21 ±7s, the bleeding time applied to the rabbit popliteal artery bleeding model is 63±11s, and the blood absorption per gram of carboxylated chitosan hemostatic particles is 5.1±1.1g, which makes it hemostasis and promote wound healing in clinical operations.

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Abstract

The invention discloses a preparation method and applications of carboxylation chitosan. The preparation method comprises following steps: a dimethylsulfoxide solution of glutaric anhydride is delivered into a sealed reactor, and is heated, chitosan is added for reaction, an obtained product is reacted in sodium hydroxide solution so as to obtain carboxylation chitosan; carboxylation chitosan is dissolved in a solid acid solution, calcium chloride is added for dissolving, an obtained mixture is subjected to spray drying so as to obtain carboxylation chitosan hemostatic particles; carboxylation chitosan is dissolved in the solid acid solution, calcium chloride is added for dissolving, the obtained mixture is subjected to freeze drying so as to obtain carboxylation chitosan hemostatic sponge; carboxylation chitosan is dissolved in an acid solution, an obtained mixed solution is injected into a mould, the mould is sealedly coated with a one-way permeable membrane, and is immersed in an alkaline medium so as to obtain chitosan gel, the obtained chitosan gel is washed with deionized water until pH value reaches 7, and is immersed in a calcium chloride solution for crosslinking, an obtained product is collected, washed, immersed in a glycerine solution, collected, and drained, is added into absolute ethyl alcohol for single-surface steaming, and is dried so as to obtain carboxylation chitosan hemostatic membranes.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a preparation method and application of carboxylated chitosan. Background technique [0002] Hemostasis has always been a major problem in hospital operations. Wound bleeding is one of the most difficult problems in surgical operations. More than half of an operation is usually spent on hemostasis, although doctors can use hemostatic forceps to stop bleeding, cauterize the wound, and even Drugs that constrict blood vessels are used, but millions of units of blood are still lost in vain on the operating table every year, and even life may be lost due to ineffective hemostasis. If the bleeding can be stopped quickly, it will not only reduce casualties, but also greatly reduce the amount of blood transfusion. At the same time, the secondary injury borne by the patient due to blood loss will also be reduced. In addition, on the battlefield, in natural disasters and i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08C08J3/24C08J3/18C08L5/08A61L15/28A61L15/42
Inventor 江春平吴俊华
Owner 江春平
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