Preparation method of antibacterial and hemostatic microballoon containing berberine and application of antibacterial hemostatic microballoon

A technology of berberine and microspheres, applied in the application, pharmaceutical formulation, pharmaceutical science and other directions, can solve the problems of unsuitable hemostatic powder, difficult to degrade and absorb, and achieve the effects of shortening hemostasis time, convenient use and long storage time.

Active Publication Date: 2019-04-05
ZHEJIANG SCI-TECH UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This kind of material hopes to use halloysite and chitosan to play the respective advantages of inorganic and organic materials to play a hemostatic and bactericidal effect, but as it says, halloysite nanotubes are inorganic materials, and the main components are silicon oxide, aluminum oxide and a small amount of other metals Oxides, such ingredients are difficult to be degraded and absorbed in the body, and cannot be hydrolyzed by lysozyme to exert a synergistic effect, so it is not suitable as a hemostatic powder for hemostasis in vivo

Method used

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  • Preparation method of antibacterial and hemostatic microballoon containing berberine and application of antibacterial hemostatic microballoon
  • Preparation method of antibacterial and hemostatic microballoon containing berberine and application of antibacterial hemostatic microballoon
  • Preparation method of antibacterial and hemostatic microballoon containing berberine and application of antibacterial hemostatic microballoon

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Experimental program
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Embodiment 1

[0039] 1. Preparation of matrix solution: 15 g sodium alginate, 5 g carboxymethyl chitosan, 0.25 g collagen and 2 g berberine were added to 1000 ml water and dissolved respectively to make 4 parts of matrix solution.

[0040] 2. Material mixing: Mix 2500 ml liquid paraffin dispersant with 180 ml Tween 80 emulsifier, add sodium alginate, carboxymethyl chitosan and collagen mixed matrix solution to emulsify and stir for 1 hour, then add 2% w / w chlorine Calcium chloride 100 ml was added dropwise to the mixed material as a cross-linking agent with rapid stirring to carry out cross-linking reaction to prepare microspheres.

[0041] 3. Refining: After the material liquid is separated, pour out the upper oil phase, discard it, add 5000ml of absolute ethanol to the lower liquid, stir, discard the supernatant, and repeat washing 3 to 5 times. Suction filtration, add 1000 ml of berberine matrix solution to dilute, then spray dry to obtain microspheres of about 1-60 μm. The microspheres...

Embodiment 2

[0043] 1. Preparation of matrix solution: 15 g of sodium alginate, 5 g of carboxymethyl chitosan, and 0.25 g of collagen were respectively dissolved in 1000 ml of water to prepare 3 parts of matrix solution.

[0044] 2. Material mixing: Mix 2500 ml liquid paraffin with 180 ml Tween 80, add sodium alginate, carboxymethyl chitosan and collagen mixed matrix solution to emulsify and stir for 1 hour, add 2% w / w calcium chloride 100 ml Add it dropwise to the mixed material with rapid stirring to carry out cross-linking reaction to prepare microspheres.

[0045] 3. Refining: After the material liquid is separated, pour out the upper oil phase, discard it, add 5000 ml of absolute ethanol to the lower liquid, stir, discard the supernatant, and repeat washing 3 to 5 times. Filter with suction, add 1000 ml of aqueous solution to dilute, and then spray dry to obtain microspheres of about 1-60 μm. The microspheres were further vacuum-dried, aseptically processed, and then sealed and packa...

Embodiment 3

[0047] 1. Preparation of matrix solution: Add 15 g of sodium alginate, 5 g of carboxymethyl chitosan, 0.25 g of collagen and 1 g of berberine into 1000 ml of water and dissolve to prepare 4 parts of matrix solution.

[0048] 2. Material mixing: Mix 2500 ml liquid paraffin dispersant with 180 ml Tween 80 emulsifier, add sodium alginate, carboxymethyl chitosan and collagen mixed matrix solution to emulsify and stir for 1 hour, then add 2% w / w chlorine Calcium chloride 100 ml was added dropwise to the mixed material as a cross-linking agent with rapid stirring to carry out cross-linking reaction to prepare microspheres.

[0049] 3. Refining: After the material liquid is separated, pour out the upper oil phase, discard it, add 5000 ml of absolute ethanol to the lower liquid, stir, discard the supernatant, and repeat washing 3 to 5 times. Suction filtration, add 1000 ml of berberine matrix solution to dilute, then spray dry to obtain microspheres of about 1-60 μm. The microspheres...

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Abstract

The invention relates to an antibacterial and hemostatic microballoon containing berberine and preparation and application of the antibacterial and hemostatic microballoon. Hemostatic can be used in abody and can be rapidly degraded and absorbed. A compound antibacterial and hemostatic material with carboxymethyl chitosan, sodium alga acid, collagen and the berberine as raw materials is adopted,the characteristics of antibacteria and adhesivity of the berberine are used, and the material is enabled to obtain the antibacterial performance while the hemostatic effect is further enhanced. Meanwhile, under the environment of simulated body liquid after hemostasis, antibacterial and hemostatic effective componenets such as the carboxymethyl chitosan, the sodium alga acid, the collagen and theberberine are degraded, the characteristics of related materials such as polysaccharides and polypeptide capable of being hydrolyzed and micromolecule being easily metabolic and decomposed are used,and thus, the effect that the antibacterial and hemostatic material can be rapidly degraded and absorbed in the body is achieved.

Description

technical field [0001] The invention relates to an antibacterial and hemostatic microsphere containing berberine, a preparation method and related applications thereof. Background of the invention [0002] Trauma bleeding is one of the common factors that induce death. Effective hemostatic materials can quickly control bleeding, which is crucial for wound treatment and obtaining time for further rescue. How to stop bleeding quickly and effectively has always been the focus and difficulty in first aid and clinical treatment, but most hemostatic agents are only effective for small blood vessel bleeding, and the hemostatic effect for large-scale and deep-seated bleeding is not ideal. The hemostatic materials on the market are mainly divided into synthetic agents, physical agents, biological agents, absorbable agents and new hemostatic dressings. Synthetic preparations, such as cyanoacrylate adhesives, etc., can quickly form high-molecular polymers in the presence of water to q...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/10A61L24/08A61L24/00
CPCA61L24/001A61L24/0015A61L24/0042A61L24/08A61L24/102A61L2300/216A61L2400/04A61L2300/418A61L2300/412A61L2300/404C08L5/08C08L5/04
Inventor 金甲叶飞吕正兵张楠姚颖洁代凯丽季志孝徐明
Owner ZHEJIANG SCI-TECH UNIV
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