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Preparation method of starch microsphere and human recombinant tissue factor lipid composite hemostatic material

A technology of starch microspheres and tissue factor, applied in drug delivery, pharmaceutical formulations, surgical adhesives, etc., can solve the problems of limited hemostatic effect of pure chitosan materials, long degradation time of cellulose materials, and increased risk of wound infection , to achieve the effect of increased specific surface area, improved water absorption performance, and shorter hemostasis time

Active Publication Date: 2021-03-12
山西省生物研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These materials have achieved good results in animal experiments and clinical applications, but there are still some shortcomings. The slow absorption of gelatin increases the risk of wound infection, animal-derived heterologous proteins are potentially allergenic, and cellulose materials take a long time to degrade. , poor adhesion, may cause rebleeding, the preparation process of Arista absorbable starch hemostatic microspheres produced by Medafor company in the United States is complicated, the price is expensive, and it is difficult to popularize. The hemostatic effect of pure chitosan material is limited. , with poor adhesion and slower hemostasis

Method used

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  • Preparation method of starch microsphere and human recombinant tissue factor lipid composite hemostatic material
  • Preparation method of starch microsphere and human recombinant tissue factor lipid composite hemostatic material

Examples

Experimental program
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Effect test

Embodiment 1

[0021] (1) Water phase preparation, add 1.5 g of potato starch into 8 mL of deionized aqueous solution, adjust the pH value to about 8.0 with NaOH solution, heat to 80 °C, wait for the starch to dissolve, and cool to room temperature.

[0022] (2) Oil phase preparation, take 1 g of Span60 into 20 mL of soybean oil, heat to 60 °C until completely dissolved, and cool to room temperature.

[0023] (3) For emulsification, slowly add the starch solution obtained in step (1) into the oil phase solution obtained in step (2), and stir thoroughly.

[0024] (4) Add 0.10 g of sodium trimetaphosphate to complete starch cross-linking and form starch microspheres.

[0025] (5) The solution in step (4) is centrifuged to separate the precipitate, washed with ethyl acetate and ethanol in sequence, and dried to obtain starch microspheres.

[0026] (6) Disperse 1.5 g of starch microspheres obtained in step (5) in 10 mL of anhydrous 1,4-dioxane solution, add 0.015 g of DSC, and 2 After protecti...

Embodiment 2

[0029] (1) Add 20 g of potato starch into 100 mL of deionized aqueous solution, adjust the pH value to about 8 with NaOH solution, heat to 80°C until the starch is dissolved, and cool to room temperature.

[0030] (2) Add 15 g of Span60 to 240 mL of soybean oil, heat to 60°C until completely dissolved, and cool to room temperature.

[0031] (3) Slowly add the starch solution obtained in step (1) into the oil phase solution obtained in step (2), and stir thoroughly.

[0032] (4) Add 1 g of sodium trimetaphosphate and stir for 5 h to complete starch cross-linking and form starch microspheres.

[0033] (5) The solution in step (4) is centrifuged to separate the precipitate, washed with ethyl acetate and ethanol in sequence, and dried to obtain starch microspheres.

[0034] (6) Disperse 20 g of the starch microspheres obtained in step (5) in 100 mL of anhydrous 1,4-dioxane solution, add 0.2 g of DSC, and 2 After protection, 4°C, and magnetic stirring for 10 h, the surface of sta...

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Abstract

The invention relates to a preparation method of a starch microsphere and human recombinant tissue factor lipid composite hemostatic material. The preparation method comprises the following steps: dissolving potato starch in deionized water, mixing with emulsified soybean oil according to the mass ratio of water phase to oil phase solution of 1: 2.0-1: 2.5, and forming starch microspheres with mutually condensed hydroxyl groups under the action of an initiator; further dispersing the obtained starch microspheres in anhydrous 1,4-dioxane, modifying with N,N'-disuccinimido carbonate (DSC), adding human recombinant tissue factor lipids, and combining the tissue factors with the starch microspheres in a covalent bonding manner. The starch microsphere and human recombinant tissue factor lipid composite hemostatic material is obtained through centrifugation, cleaning and vacuum drying, and a rapid blood coagulation effect is achieved by utilizing the starting blood coagulation function of tissue factors.

Description

technical field [0001] The field relates to a hemostatic compound, which is a rapid hemostatic dry powder material for external use. Background technique [0002] Accidental bleeding and surgical bleeding, improper handling can cause shock and even death. The clinical cases caused by excessive blood loss account for a large proportion. Hemostasis is the core link of treatment, and good hemostasis technology is the guarantee of success in this link. The development and improvement of hemostatic materials has become a research hotspot in the field of contemporary medicine. With the advancement of medical technology, new medical materials and related technologies have been well developed. [0003] The effective hemostatic material is to use absorbable material with good biocompatibility to stop bleeding, which is safe, non-allergic and non-irritating. At present, the hemostatic materials on the market mainly include fibrin glue, collagen, oxidized cellulose hemostatic gauze, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/08A61L24/00
CPCA61L24/08A61L24/001A61L24/0042A61L2400/04C08L3/02
Inventor 芦冬涛赵峰梅李红霞赵邑
Owner 山西省生物研究院有限公司
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