60 results about "Succinamopine" patented technology

The invention discloses a preparation method of an optical OCA (Optical Clear Adhesive) and application of the optical OCA in a mobile phone folding screen, and relates to the technical field of optical cement, the optical OCA is prepared from the following raw materials in parts by weight: 50 parts of acrylate prepolymers, 2-4 parts of 3-allyl-2-mercapto-3H-quinazoline-4-ketone, 1-3 parts of diallyl disulfide, 1-3 parts of 3-[N, N-dimethyl formamide], 1-3 parts of 2, 3, 5-trimethyl-1, 3-pentanediol monoisobutyronitrile, 1-3 parts of 2, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3 The preparation method comprises the following steps: adding 3-5 parts of N, N-dimethyl-[2-(2-methylpropane-2-enoyloxy) ethyl] ammonium] propane-1-sulfonic acid inner salt, 1-3 parts of allyl succinimido carbonate, 2-4 parts of dicyclopentene methacrylate, 1-3 parts of 1, 3, 5-triacryloyl hexahydro-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5- The invention relates to a graphene quantum dot photoinitiator which is prepared from the following components in parts by weight: 1-3 parts of 1, 3, 5-triazine, 0.8-1.2 parts of a photoinitiator, 1-3 parts of an aziridine cross-linking agent, 1-3 parts of graphene quantum dots, 0.8-1.2 parts of a coupling agent and 40-50 parts of a diluent. The optical OCA adhesive is high in light transmittance and bonding strength, good in weather resistance and excellent in folding resistance.

The invention provides a polyethylene glycol derivative-collagen cartilage repair gel which is characterized by being prepared from the following components in parts by weight: 8 to 12 parts of collagen (namely COL) solution, 0.5 to 2 parts of polydeoxyribonucleotide (namely PDRN), 4 to 8 parts of polyethylene glycol disuccinimide succinate (namely SS-PEG-SS) solution and 0.1 to 0.4 part of tannic acid (namely TA), and particularly discloses a preparation method and application of the polyethylene glycol derivative-collagen cartilage repair gel. In a word, the composite gel and the collagen are subjected to efficient amidation reaction through SS-PEG-SS to form a stable three-dimensional network, so that the structural support of the type I collagen, the tissue repair of PDRN and the anti-inflammatory and anti-oxidation characteristics of tannic acid are successfully integrated; therefore, the composite material has the comprehensive performance of good structural stability, high mechanical strength, good biocompatibility, controllable degradation period, excellent needle passing performance and the like, and shows the wide application prospects of clear repair effect, remarkable component synergistic effect, mature production process, outstanding cost effectiveness and the like.

The invention discloses periodontitis targeted sustained-release gel based on epithelial training immunization and a preparation method thereof, and relates to the technical field of biological medicine, and the preparation method comprises the following steps: modifying poloxamer, and dissolving the modified poloxamer in anhydrous acetonitrile; the preparation method comprises the following steps: dissolving RGD peptide in a PBS (Phosphate Buffer Solution), adding N-hydroxysuccinimide and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide, and activating to obtain an activated RGD peptide solution; adding the activated RGD peptide solution into the modified poloxamer solution, and reacting at room temperature to obtain RGD peptide modified poloxamer; the RGD peptide modified poloxamer solution is mixed with a beta-glucan solution and a TLR2 agonist solution, and the periodontitis targeted slow-release gel is obtained. The gel provided by the invention specifically targets gingival epithelial cells, reduces inflammatory response and promotes periodontal tissue regeneration, has good biocompatibility and slow release performance, and can effectively control periodontitis and prevent relapse.

The invention relates to the technical field of fibers, in particular to a PHBV-containing bio-based high-strength antibacterial fiber and a preparation method thereof.The fiber is obtained by conducting blending and electrostatic spinning on environment-friendly materials including silk fibroin, polyoxyethylene, PHBV and a composite natural antibacterial agent; 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride, N-hydroxysuccinimide, a composite natural antibacterial agent and carboxymethyl chitosan are used as raw materials to prepare a cross-linking steeping liquor, and the electrostatic spinning fibers are subjected to cross-linking modification treatment in the cross-linking steeping liquor; lignin and hexadecyl trimethyl ammonium bromide are combined for electrostatic assembly to coat a natural antibacterial agent to serve as a pretreatment natural antibacterial agent, 3-chloropropyl-trimethoxysilane serves as a coupling agent, ethylenediamine serves as an amination modification reagent, an amination natural antibacterial agent is obtained, and then a bio-based bacterial adhesion prevention flame-retardant polymer is grafted.

The invention discloses a preparation method of a carbon fiber-iodine doped carbon quantum dot composite material based on surface modification and chemical crosslinking, and belongs to the technical field of nano composite materials. The material is prepared by carrying out surface treatment on carbon fibers to introduce functional groups, carrying out iodine doping on carbon quantum dots, and directionally connecting complementary functional groups of the carbon fibers and the carbon quantum dots by adopting a bifunctional crosslinking agent (such as 1-ethyl-3-(3-dimethyl propyl) carbodiimide (EDC) / N-hydroxysuccinimide (NHS), glutaraldehyde, hexamethylene diisocyanate and toluene diisocyanate). A stable composite interface is formed through covalent bonds. According to the method, the high CT contrast ratio of the iodine-doped carbon quantum dots is utilized, and in the using process of the carbon fiber, damage and defects in the carbon fiber structure can be detected through a CT imaging method.

The invention belongs to the field of protein hydrogel, and relates to protein adhesive hydrogel for promoting diabetic oral soft tissue injury repair and a preparation method of the protein adhesive hydrogel. The hydrogel is prepared by mixing an aqueous solution of recombinant fusion protein and an aqueous solution of polyethylene glycol bissuccinimide succinate. The sequence of the recombinant fusion protein is as shown in SEQ ID NO: 1: [PAATAVSHTTHAP (VPGKG) 5] 36. The hydrogel disclosed by the invention has good biocompatibility and excellent adhesion performance, can form a lasting antibacterial barrier, and is electrostatically combined with free DNA (Deoxyribonucleic Acid) in NETs, so that inflammatory factor expression induced by the NETs is inhibited, and finally diabetes wound healing is accelerated.

The application relates to the field of biological materials, and provides an anti-freezing hemostatic gel material and a preparation method thereof, which comprises the following steps: S1, preparing an EA complex; the EA complex is prepared by reacting epigallocatechin gallate and 3-acrylamide phenylboronic acid; S2, preparing a gel precursor solution: taking methylacrylated gelatin and acrylic acid in deionized water and heating to completely dissolve; adding an anti-freezing complex and uniformly mixing; subsequently, adding acrylic acid N-succinimidyl ester to obtain the gel precursor solution; the anti-freezing complex is prepared by mixing nanocellulose, proline and glycerol; S3, preparing an anti-freezing hemostatic gel material: mixing and reacting the EA complex, the gel precursor solution, acrylic acid, an initiator and an accelerator to prepare the anti-freezing hemostatic gel material. The anti-freezing hemostatic gel material has good adhesion and high mechanical strength, and can well adapt to the use requirements in a plateau environment.

The invention discloses an endoplasmic reticulum targeted lipid peroxidation fluorescent indicator and application thereof, and the indicator introduces a conjugated diolefin structure as a lipid peroxidation recognition unit to obtain a series of BODIPY derivatives with the maximum emission wavelength of 620 nm. A conjugated diene part of the fluorescent probe can be subjected to oxidative cracking under the action of reactive oxygen species, and a conjugated system of the fluorescent probe becomes smaller due to breakage of double bonds, which shows that absorption and emission wavelengths are obviously shortened. Therefore, the LPO process can be monitored through the change of the fluorescence ratio. Meanwhile, hydrophilic succinimide ester sulfonic acid group NHS is introduced as a group reacting with protein, the oil-water coefficient of the compound is moderate, the compound easily penetrates through a cell membrane and reacts with adjacent protein free amino in a short time, the probe can be fixed on the protein of an endoplasmic reticulum through a chemical bond, and the probe can be used for long-time fluorescence imaging research. And long-time real-time tracking is carried out on the movement and form change of the endoplasmic reticulum.

Provided is a hydrogel precursor polymer derived from a poly (asparagine) (PAsPm) comprising cleavable and crosslinkable moieties attached to a pendant carboxylic acid group of the poly (asparagine); and a hydrophilic moiety also attached to a pendant carboxylic acid group, such as an ether-containing moiety obtained by ring opening a polysuccinimide with 2-(2-aminoethoxy) ethanol.

The application discloses a long-acting developing medical hydrogel and a preparation method thereof, and belongs to the field of biomedical materials. The long-acting developing medical hydrogel comprises a first component and a second component. The first component comprises multi-arm developing agent amine salt, succinimidyl ester-terminated multi-arm polyethylene glycol and a diluent. The second component is an accelerator solution. The second component is mixed with the first component to form the long-acting developing medical hydrogel through in-situ crosslinking. The long-acting developing medical hydrogel makes up for the shortcoming that conventional hydrogels cannot realize CT developing imaging in the body for a long time. The strength of the hydrogel can be adjusted by adjusting the arm number of the multi-arm developing agent amine salt under the condition that the concentration is unchanged. The multi-arm developing agent amine salt and the succinimidyl ester-terminated multi-arm polyethylene glycol can prolong the effective use time of the succinimidyl ester-terminated multi-arm polyethylene glycol after being mixed, thereby providing sufficient operation time for the operation of a clinician. In addition, the multi-arm developing agent amine salt can resist conventional Co60 irradiation sterilization.

The invention discloses a preparation method of a water treatment agent and the water treatment agent. The preparation method adopts a one-step method for synthesis, and comprises the following steps: mixing polysuccinimide, benzisothiazolinone and alkali, and then adding a solvent for reaction to obtain the water treatment agent. The water treatment agent prepared by the preparation method disclosed by the invention has the effects of scale inhibition and sterilization at the same time, and the efficiency of the water treatment process is improved.

This application belongs to the field of metal corrosion inhibition, specifically providing a neutralizing corrosion inhibitor and its preparation method. A neutralizing corrosion inhibitor comprises the following components by weight: 50-60 parts deionized water, 18-20 parts corrosion inhibitor matrix, 10-14 parts triethanolamine, 3-5 parts benzotriazole, and 6-8 parts ethylene glycol. The corrosion inhibitor matrix of this application is made from raw materials including polysuccinimide, triaminopyrimidine, and tryptophan derivatives. The neutralizing corrosion inhibitor prepared by this application exhibits good thermal stability and excellent corrosion inhibition performance.

Provided are antibody-drug conjugates, methods for preparing them, and uses thereof. The antibody-drug conjugates comprise an antibody, a cytotoxic payload, and a linker of Formula I, wherein the succinimidyl group of the linker of Formula I forms a thioether bond with a thiol group obtained by reduction of the interchain disulfide chain of the antibody, and the carbonyl group in the ester group of the linker is bonded to the amino group of the payload. The antibody-drug conjugates are used in the manufacture of therapeutic agents for the diagnosis, prevention, and treatment of neoplastic diseases.

The invention relates to tear-resistant plush cloth. A composite coating is arranged on the surface of a plush cloth main body; the composite coating comprises a polyacrylate layer and a modified layer, wherein the modified layer is prepared from the following components: phthaloyl glycine hydroxysuccinimide ester, benzoyl peroxide, cyclopentene-1-yl boric acid, a carbon nano tube, dimethyl sulfoxide and deionized water; the invention further relates to a preparation method of the tear-resistant plush fabric. The preparation method comprises the steps of preparation of the modified layer coating liquid and dip-coating operation. By arranging the double-layer composite coating and polyacrylate, the tear resistance, the waterproof function, the antifouling function and the like are improved; the preparation method comprises the following steps: carrying out graft copolymerization on a modified layer which takes phthaloyl glycine hydroxysuccinimide ester as a main material and cyclopentene-1-yl boric acid under the action of benzoyl peroxide; and free radicals decomposed by benzoyl peroxide can attack polyester fiber polyester chains of the plush cloth and are subjected to graft copolymerization with cyclopentene-1-yl boric acid to obtain derivatives carrying boric acid groups, and the derivatives and a polyacrylate layer synergistically improve the tear resistance.

The invention provides a preparation method of N-(4-(tert-butyl) thiazole-2-yl)-3-fluorobenzamide, which comprises the following steps: mixing a compound as shown in a formula I, N, N-diisopropylethylamine and 2-succinimido-1, 1, 3, 3-tetramethylurea tetrafluoroborate for reaction, then adding a compound as shown in a formula II for amidation reaction, and finally obtaining the N-(4-(tert-butyl) thiazole-2-yl)-3-fluorobenzamide. And reacting the N-(4-(tert-butyl) thiazole-2-yl)-3-fluorobenzamide to obtain the N-(4-(tert-butyl) thiazole-2-yl)-3- According to the preparation method provided by the invention, by innovatively changing the reaction sequence, firstly activating the raw materials and then carrying out amidation reaction, the polarity of by-products is successfully changed, the polarity of main by-products is obviously smaller than that of target products, the products are easy to separate, the column chromatography separation efficiency is greatly improved, the yield is increased, and the post-treatment time is shortened.

This invention discloses a wound dressing with photothermal antibacterial, hemocoagulant-promoting, and easily removable properties, its preparation method, and applications, belonging to the field of biomaterials technology. The method includes dissolving sericin in water, then sequentially adding hemoglobin, carbodiimide, and N-hydroxysuccinimide to induce a cross-linking reaction; obtaining a precursor for the composite protein dressing through a mechanochemical cross-linking strategy; pre-cooling the precursor and freeze-drying it to obtain a sericin-hemoglobin dressing; dialyzing the sericin-hemoglobin dressing in water for 2-3 days, and freeze-drying it again to obtain a protein dressing containing only hemoglobin and sericin. The antibacterial dressing prepared by this invention, upon contact with blood, promotes the formation of a dense fibrin membrane, promoting hemostasis while making it easy to remove from the wound site. This wound dressing is entirely composed of protein, exhibits good biocompatibility, and has great development potential.

The application belongs to the technical field of lithium ion batteries, and discloses a carbon-compatible binder for a silicon-carbon negative electrode of a lithium ion battery, a preparation method and application thereof, wherein the carbon-compatible binder is abbreviated as CMC-Py, sodium carboxymethyl cellulose is dissolved in a phosphate buffer solution, then N-hydroxysuccinimide and 1-ethyl-(3-dimethylaminopropyl) carbodiimide are added to activate the carboxyl group of the sodium carboxymethyl cellulose, finally, 1-pyrenemethylamine hydrochloride is added, and the reaction is carried out under stirring and N2 at room temperature to obtain the binder. The binder is prepared by the amidation reaction of sodium carboxymethyl cellulose as a main chain and 1-pyrenemethylamine hydrochloride, the pyrene group introduced can form a pi-pi conjugated system with graphite in the silicon-carbon material, the interface compatibility of the binder and the silicon-carbon material can be effectively improved, the dispersibility and the adhesion of the silicon-carbon material can be enhanced, and the electrochemical performance of the silicon-carbon negative electrode of the lithium ion battery is significantly improved.

The invention discloses multifunctional hemostatic cryogel with orientation channels and a preparation method and application of the multifunctional hemostatic cryogel. Relates to the field of medical materials. The hemostasis cryogel has a columnar-like structure, and an axial orientation channel and a radial orientation channel are arranged in the hemostasis cryogel; the axial channels are arranged in a tubular mode, and the radial channels are of a honeycomb-like porous structure and communicate with the side face and the interior of the columnar-like structure. The hemostatic cryogel comprises the following components: modified polyethylene glycol, and protein or a derivative thereof, the modified polyethylene glycol is modified with an R group, and the R group comprises at least one of carboxyl, amino, aldehyde group, sulfydryl, alkylene and succinimide active ester. When the hemostatic cryogel is used, the hemostatic cryogel can be directly injected to a wound surface, rapidly absorbs liquid and expands after being in contact with blood or tissue fluid, fills a wound cavity and forms stable plugging, so that rapid hemostasis is realized.

The invention discloses a wound dressing capable of preventing seawater soaking. The wound dressing is prepared from a dressing gel material and a polyurethane (PU) film, the dressing gel material is prepared from the following components: chitosan, freeze-dried A-S-Pvfp5-P recombinant protein, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, N-hydroxysuccinimide and a double-network hydrogel material, and the double-network hydrogel material is prepared from the chitosan, the freeze-dried A-S-Pvfp5-P recombinant protein, the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and the N-hydroxysuccinimide. The seawater soaking prevention wound dressing provided by the invention solves the problems of insufficient seawater soaking prevention effect, poor adhesion performance and poor wound closing effect in the prior art.

The application belongs to the technical field of micro-robot preparation, and provides a magnetic micro-robot with ROS response and shape self-adaptability, a preparation method and application thereof. The preparation method comprises the following steps: mixing eight-arm polyethylene diamine, eight-arm succinimidyl carboxylate, iron oxide nanoparticles, antioxidant material, cell adhesion molecule and PBS buffer to obtain a mixture; mixing the mixture with n-hexane, liquid paraffin and span 80, and performing magnetic stirring, and then standing to obtain a magnetic micro-robot mixture; and separating the magnetic micro-robot mixture by using a permanent magnet to obtain magnetic gel microspheres, i.e. the magnetic micro-robot. The magnetic micro-robot prepared by the application has ROS response and good shape self-adaptability, and can be widely applied to the medical field.

The invention discloses a graphene anticorrosive coating, a preparation method thereof and a refrigerator, and belongs to the technical field of non-ferrous metal protection. The graphene anticorrosive coating is composed of benzidine-disuccinimido suberic acid ester modified graphene, and the benzidine-disuccinimido suberic acid ester is combined with the graphene through a pi-pi conjugation effect. The graphene anticorrosive coating is applied to protection of the copper electrode for the refrigerator, the problem that an existing graphene coating for corrosion prevention of the copper electrode cannot give consideration to corrosion prevention performance and conductivity is solved, and the graphene anticorrosive coating has good toughness, excellent corrosion prevention effect and high conductivity.

The invention relates to an extracellular vesicle in-situ microreactor and preparation and application thereof, the inner wall of a capillary is subjected to silanization treatment, and the surface of the capillary is modified with epoxy groups and amphoteric molecules through free radical polymerization reaction, so that a capillary open tubular column is prepared; and then modifying polyethyleneimine and iodoacetic acid N-hydroxysuccinimide ester in sequence through covalent bonding to prepare the amphoteric solid-phase alkylation capillary microreactor. The method has the advantages that the extracellular vesicles are captured in situ, the vesicle proteome sample is treated, and the method is suitable for proteome sample treatment of trace extracellular vesicle samples. In addition, the method is resistant to various surfactants and strong cracking reagents and has good sample compatibility.

The invention discloses a ready-to-use sodium alginate paper-coated material for three-dimensional cell culture as well as a preparation method and application thereof, and belongs to the technical field of cell culture. The preparation method of the ready-to-use sodium alginate-coated paper material comprises the following steps: dropwise adding sodium alginate onto a paper material, cross-linking sodium alginate hydrogel through polyethylene glycol-diamine, 1-ethyl-(3-dimethylaminopropyl)-carbodiimide hydrochloride and N-hydroxysuccinimide, and freeze-drying to prepare the ready-to-use sodium alginate-coated paper material. Compared with the traditional hydrogel, the problems that the prepared hydrogel is difficult to dissolve, bubbles are easily generated when the hydrogel is mixed with cells, and the bubbles are difficult to eliminate are effectively avoided; besides, the ready-to-use sodium alginate and paper material has no cytotoxicity, has good stability and can be stored for 120 days at room temperature in a dark state, and compared with the currently prepared and used sodium alginate and paper material, the ready-to-use sodium alginate and paper material has no obvious difference in cell adhesion capability and cell three-dimensional culture capability.

The application belongs to the field of antitumor drug preparation, and particularly relates to albumin binding and non-binding gemcitabine prodrugs, and a preparation method and application thereof. The acryloyl chloride gemcitabine prodrug is a compound formed by bridging gemcitabine and acryloyl chloride through an amide bond, and the two non-albumin binding prodrugs are compounds formed by bridging gemcitabine and 3-succinimidyl propionic acid / propionic acid through an amide bond. The ethylene group in the prodrug is a binding target for the free thiol group of the 34th cysteine on albumin, can be rapidly and specifically combined with albumin in the systemic circulation, and further forms an albumin prodrug complex. The complex can significantly improve the stability of gemcitabine in the systemic circulation, and prolong the systemic circulation time. The macromolecular prodrug strategy designed in the application can improve the delivery efficiency of chemotherapeutic drugs, improve the antitumor effect of chemotherapeutic drugs, and provides a new direction and new ideas for the delivery of chemotherapeutic drugs.

The invention discloses long-acting developing medical hydrogel and a preparation method thereof, and belongs to the field of biomedical materials.The long-acting developing medical hydrogel comprises a first component and a second component; the first component comprises a multi-arm developer amine salt, succinimide ester terminated multi-arm polyethylene glycol and a diluent; the second component is an accelerator solution; the second component and the first component are mixed and then cross-linked in situ to form the long-acting developing medical hydrogel, so that the defect that conventional hydrogel cannot realize CT developing imaging in vivo for a long time is overcome, and the strength of the hydrogel can be adjusted by adjusting the arm number of amine salt of a multi-arm developing agent under the condition that the concentration is not changed; meanwhile, after the multi-arm developing agent amine salt and the succinimide ester end-capped multi-arm polyethylene glycol are mixed, the effective use time of the succinimide ester end-capped multi-arm polyethylene glycol can be prolonged, sufficient operation time is provided for surgical operation of clinicians, and in addition, the multi-arm developing agent amine salt can tolerate conventional Co60 irradiation sterilization.

The invention relates to the technical field of pet supplies, in particular to coal gangue cat litter and a preparation method thereof. The coal gangue cat litter comprises the following components: activated coal gangue and macromolecular organic matters; the activated coal gangue is obtained by activating an oxidizing agent; the macromolecular organic matter is selected from at least one of polyacrylamide, polyethylene glycol, maleimide, suberic acid disuccinimide ester, carbodiimide, agar, xanthan gum, gelatin, pectin, Arabic gum and guar gum. The invention also provides a preparation method of the coal gangue cat litter, which comprises the following steps: crushing the coal gangue into powder, adding water and an oxidizing agent, adding the macromolecular organic matter and the acid into the obtained activated coal gangue to obtain micelles, and granulating to obtain the coal gangue cat litter. The defects that existing cat litter is insufficient in strength and prone to forming dust due to mutual friction are overcome, and the problems that existing cat litter products lack timely feedback on health conditions of urinary and digestive systems of pet cats, and the existing cat litter products are high in raw material cost and prone to generating peculiar smells are solved.

The invention discloses a supramolecular polymer based on lipoic acid-based polydimethylsiloxane as well as an application and a degradation method of the supramolecular polymer. The supramolecular polymer is prepared by synthesizing an intermediate from lipoic acid and derivatives thereof and N, N '-succinimido carbonate, and then reacting with polydimethylsiloxane with different chain lengths and viscosities. The synthesis is simple, the raw material cost is low, and industrial production is easy. The polymer can be processed into different structures, is used as a dielectric layer of a capacitive sensor, and has good performance. Besides, the polymer can be degraded in a chloroform solution containing benzyl mercaptan and 1, 8-diazabicyclo [5.4. 0] undec-7-ene, and can be completely dissolved within 48 hours, so that the problems of complex synthesis, high cost and non-degradability of the existing material are solved.

The application relates to a preparation and application method of a novel crosslinking agent enrichment based on a sulfur-fluorine exchange reaction (SuFEx), and belongs to the technical field of organic synthesis. The application is based on the existing trimethylpiperidyl bis-succinimidyl ester crosslinking agent, and a series of crosslinking agents with a single end or a double end of benzylsulfonyl fluoride are synthesized based on the SuFEx reaction. The crosslinking agent has the characteristics of enrichment, adjustable arm length, multiple reaction sites, isotope quantification and the like, not only improves the crosslinking depth and coverage, but also can realize the relative quantification of proteins. The application provides important technical support for in-situ protein analysis based on a chemical crosslinking strategy.

The invention relates to a preparation method of coupling magnetic beads for microbial collection, which adopts SPDP (N-succinimido-3-(2-pyridine dithio) propionate) as a cross-linking agent to realize directional fixation of recombinant protein A-Cys on magnetosome, and then the recombinant protein A-Cys can be coupled with an antibody to form the coupling magnetic beads for microbial collection with specific antigen binding. The coupling magnetic bead for microbial collection prepared by the preparation method provided by the invention can be efficiently coupled with different types of antibodies, and has a relatively wide application prospect.

The application discloses an alkaline phosphatase antibody label and a preparation method, a detection reagent and a kit thereof, relates to the field of protein coupling technology. The alkaline phosphatase antibody label provided by the technical scheme of the application is prepared by activating alkaline phosphatase by a polyethylene glycol compound containing a succinimidyl ester group and SMCC, or is prepared by modifying alkaline phosphatase by a polyethylene glycol molecule with an amino group and then activating by SMCC. Compared with the traditional Traut's-SMCC method, the alkaline phosphatase antibody label has improved hydrophilicity, improved solubility, and certain steric hindrance, so that the possibility of continued reaction of the excess active sites on the alkaline phosphatase can be reduced, the strength of the cross-linking and aggregation of the alkaline phosphatase and the antibody can be reduced without affecting the cross-linking efficiency, so that the stability and yield of the alkaline phosphatase antibody label are improved, and batch differences are reduced.