42 results about "Succinamopine" patented technology

The invention provides a polyethylene glycol derivative-collagen cartilage repair gel which is characterized by being prepared from the following components in parts by weight: 8 to 12 parts of collagen (namely COL) solution, 0.5 to 2 parts of polydeoxyribonucleotide (namely PDRN), 4 to 8 parts of polyethylene glycol disuccinimide succinate (namely SS-PEG-SS) solution and 0.1 to 0.4 part of tannic acid (namely TA), and particularly discloses a preparation method and application of the polyethylene glycol derivative-collagen cartilage repair gel. In a word, the composite gel and the collagen are subjected to efficient amidation reaction through SS-PEG-SS to form a stable three-dimensional network, so that the structural support of the type I collagen, the tissue repair of PDRN and the anti-inflammatory and anti-oxidation characteristics of tannic acid are successfully integrated; therefore, the composite material has the comprehensive performance of good structural stability, high mechanical strength, good biocompatibility, controllable degradation period, excellent needle passing performance and the like, and shows the wide application prospects of clear repair effect, remarkable component synergistic effect, mature production process, outstanding cost effectiveness and the like.

The invention belongs to the field of protein hydrogel, and relates to protein adhesive hydrogel for promoting diabetic oral soft tissue injury repair and a preparation method of the protein adhesive hydrogel. The hydrogel is prepared by mixing an aqueous solution of recombinant fusion protein and an aqueous solution of polyethylene glycol bissuccinimide succinate. The sequence of the recombinant fusion protein is as shown in SEQ ID NO: 1: [PAATAVSHTTHAP (VPGKG) 5] 36. The hydrogel disclosed by the invention has good biocompatibility and excellent adhesion performance, can form a lasting antibacterial barrier, and is electrostatically combined with free DNA (Deoxyribonucleic Acid) in NETs, so that inflammatory factor expression induced by the NETs is inhibited, and finally diabetes wound healing is accelerated.

The application relates to the field of biological materials, and provides an anti-freezing hemostatic gel material and a preparation method thereof, which comprises the following steps: S1, preparing an EA complex; the EA complex is prepared by reacting epigallocatechin gallate and 3-acrylamide phenylboronic acid; S2, preparing a gel precursor solution: taking methylacrylated gelatin and acrylic acid in deionized water and heating to completely dissolve; adding an anti-freezing complex and uniformly mixing; subsequently, adding acrylic acid N-succinimidyl ester to obtain the gel precursor solution; the anti-freezing complex is prepared by mixing nanocellulose, proline and glycerol; S3, preparing an anti-freezing hemostatic gel material: mixing and reacting the EA complex, the gel precursor solution, acrylic acid, an initiator and an accelerator to prepare the anti-freezing hemostatic gel material. The anti-freezing hemostatic gel material has good adhesion and high mechanical strength, and can well adapt to the use requirements in a plateau environment.

Provided is a hydrogel precursor polymer derived from a poly (asparagine) (PAsPm) comprising cleavable and crosslinkable moieties attached to a pendant carboxylic acid group of the poly (asparagine); and a hydrophilic moiety also attached to a pendant carboxylic acid group, such as an ether-containing moiety obtained by ring opening a polysuccinimide with 2-(2-aminoethoxy) ethanol.

The application discloses a long-acting developing medical hydrogel and a preparation method thereof, and belongs to the field of biomedical materials. The long-acting developing medical hydrogel comprises a first component and a second component. The first component comprises multi-arm developing agent amine salt, succinimidyl ester-terminated multi-arm polyethylene glycol and a diluent. The second component is an accelerator solution. The second component is mixed with the first component to form the long-acting developing medical hydrogel through in-situ crosslinking. The long-acting developing medical hydrogel makes up for the shortcoming that conventional hydrogels cannot realize CT developing imaging in the body for a long time. The strength of the hydrogel can be adjusted by adjusting the arm number of the multi-arm developing agent amine salt under the condition that the concentration is unchanged. The multi-arm developing agent amine salt and the succinimidyl ester-terminated multi-arm polyethylene glycol can prolong the effective use time of the succinimidyl ester-terminated multi-arm polyethylene glycol after being mixed, thereby providing sufficient operation time for the operation of a clinician. In addition, the multi-arm developing agent amine salt can resist conventional Co60 irradiation sterilization.

This application belongs to the field of metal corrosion inhibition, specifically providing a neutralizing corrosion inhibitor and its preparation method. A neutralizing corrosion inhibitor comprises the following components by weight: 50-60 parts deionized water, 18-20 parts corrosion inhibitor matrix, 10-14 parts triethanolamine, 3-5 parts benzotriazole, and 6-8 parts ethylene glycol. The corrosion inhibitor matrix of this application is made from raw materials including polysuccinimide, triaminopyrimidine, and tryptophan derivatives. The neutralizing corrosion inhibitor prepared by this application exhibits good thermal stability and excellent corrosion inhibition performance.

The invention provides a preparation method of N-(4-(tert-butyl) thiazole-2-yl)-3-fluorobenzamide, which comprises the following steps: mixing a compound as shown in a formula I, N, N-diisopropylethylamine and 2-succinimido-1, 1, 3, 3-tetramethylurea tetrafluoroborate for reaction, then adding a compound as shown in a formula II for amidation reaction, and finally obtaining the N-(4-(tert-butyl) thiazole-2-yl)-3-fluorobenzamide. And reacting the N-(4-(tert-butyl) thiazole-2-yl)-3-fluorobenzamide to obtain the N-(4-(tert-butyl) thiazole-2-yl)-3- According to the preparation method provided by the invention, by innovatively changing the reaction sequence, firstly activating the raw materials and then carrying out amidation reaction, the polarity of by-products is successfully changed, the polarity of main by-products is obviously smaller than that of target products, the products are easy to separate, the column chromatography separation efficiency is greatly improved, the yield is increased, and the post-treatment time is shortened.

This invention discloses a wound dressing with photothermal antibacterial, hemocoagulant-promoting, and easily removable properties, its preparation method, and applications, belonging to the field of biomaterials technology. The method includes dissolving sericin in water, then sequentially adding hemoglobin, carbodiimide, and N-hydroxysuccinimide to induce a cross-linking reaction; obtaining a precursor for the composite protein dressing through a mechanochemical cross-linking strategy; pre-cooling the precursor and freeze-drying it to obtain a sericin-hemoglobin dressing; dialyzing the sericin-hemoglobin dressing in water for 2-3 days, and freeze-drying it again to obtain a protein dressing containing only hemoglobin and sericin. The antibacterial dressing prepared by this invention, upon contact with blood, promotes the formation of a dense fibrin membrane, promoting hemostasis while making it easy to remove from the wound site. This wound dressing is entirely composed of protein, exhibits good biocompatibility, and has great development potential.

The application belongs to the technical field of lithium ion batteries, and discloses a carbon-compatible binder for a silicon-carbon negative electrode of a lithium ion battery, a preparation method and application thereof, wherein the carbon-compatible binder is abbreviated as CMC-Py, sodium carboxymethyl cellulose is dissolved in a phosphate buffer solution, then N-hydroxysuccinimide and 1-ethyl-(3-dimethylaminopropyl) carbodiimide are added to activate the carboxyl group of the sodium carboxymethyl cellulose, finally, 1-pyrenemethylamine hydrochloride is added, and the reaction is carried out under stirring and N2 at room temperature to obtain the binder. The binder is prepared by the amidation reaction of sodium carboxymethyl cellulose as a main chain and 1-pyrenemethylamine hydrochloride, the pyrene group introduced can form a pi-pi conjugated system with graphite in the silicon-carbon material, the interface compatibility of the binder and the silicon-carbon material can be effectively improved, the dispersibility and the adhesion of the silicon-carbon material can be enhanced, and the electrochemical performance of the silicon-carbon negative electrode of the lithium ion battery is significantly improved.

The invention discloses multifunctional hemostatic cryogel with orientation channels and a preparation method and application of the multifunctional hemostatic cryogel. Relates to the field of medical materials. The hemostasis cryogel has a columnar-like structure, and an axial orientation channel and a radial orientation channel are arranged in the hemostasis cryogel; the axial channels are arranged in a tubular mode, and the radial channels are of a honeycomb-like porous structure and communicate with the side face and the interior of the columnar-like structure. The hemostatic cryogel comprises the following components: modified polyethylene glycol, and protein or a derivative thereof, the modified polyethylene glycol is modified with an R group, and the R group comprises at least one of carboxyl, amino, aldehyde group, sulfydryl, alkylene and succinimide active ester. When the hemostatic cryogel is used, the hemostatic cryogel can be directly injected to a wound surface, rapidly absorbs liquid and expands after being in contact with blood or tissue fluid, fills a wound cavity and forms stable plugging, so that rapid hemostasis is realized.

The invention discloses a wound dressing capable of preventing seawater soaking. The wound dressing is prepared from a dressing gel material and a polyurethane (PU) film, the dressing gel material is prepared from the following components: chitosan, freeze-dried A-S-Pvfp5-P recombinant protein, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, N-hydroxysuccinimide and a double-network hydrogel material, and the double-network hydrogel material is prepared from the chitosan, the freeze-dried A-S-Pvfp5-P recombinant protein, the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and the N-hydroxysuccinimide. The seawater soaking prevention wound dressing provided by the invention solves the problems of insufficient seawater soaking prevention effect, poor adhesion performance and poor wound closing effect in the prior art.

The invention discloses a graphene anticorrosive coating, a preparation method thereof and a refrigerator, and belongs to the technical field of non-ferrous metal protection. The graphene anticorrosive coating is composed of benzidine-disuccinimido suberic acid ester modified graphene, and the benzidine-disuccinimido suberic acid ester is combined with the graphene through a pi-pi conjugation effect. The graphene anticorrosive coating is applied to protection of the copper electrode for the refrigerator, the problem that an existing graphene coating for corrosion prevention of the copper electrode cannot give consideration to corrosion prevention performance and conductivity is solved, and the graphene anticorrosive coating has good toughness, excellent corrosion prevention effect and high conductivity.

The invention relates to an extracellular vesicle in-situ microreactor and preparation and application thereof, the inner wall of a capillary is subjected to silanization treatment, and the surface of the capillary is modified with epoxy groups and amphoteric molecules through free radical polymerization reaction, so that a capillary open tubular column is prepared; and then modifying polyethyleneimine and iodoacetic acid N-hydroxysuccinimide ester in sequence through covalent bonding to prepare the amphoteric solid-phase alkylation capillary microreactor. The method has the advantages that the extracellular vesicles are captured in situ, the vesicle proteome sample is treated, and the method is suitable for proteome sample treatment of trace extracellular vesicle samples. In addition, the method is resistant to various surfactants and strong cracking reagents and has good sample compatibility.

The application belongs to the field of antitumor drug preparation, and particularly relates to albumin binding and non-binding gemcitabine prodrugs, and a preparation method and application thereof. The acryloyl chloride gemcitabine prodrug is a compound formed by bridging gemcitabine and acryloyl chloride through an amide bond, and the two non-albumin binding prodrugs are compounds formed by bridging gemcitabine and 3-succinimidyl propionic acid / propionic acid through an amide bond. The ethylene group in the prodrug is a binding target for the free thiol group of the 34th cysteine on albumin, can be rapidly and specifically combined with albumin in the systemic circulation, and further forms an albumin prodrug complex. The complex can significantly improve the stability of gemcitabine in the systemic circulation, and prolong the systemic circulation time. The macromolecular prodrug strategy designed in the application can improve the delivery efficiency of chemotherapeutic drugs, improve the antitumor effect of chemotherapeutic drugs, and provides a new direction and new ideas for the delivery of chemotherapeutic drugs.

The invention discloses long-acting developing medical hydrogel and a preparation method thereof, and belongs to the field of biomedical materials.The long-acting developing medical hydrogel comprises a first component and a second component; the first component comprises a multi-arm developer amine salt, succinimide ester terminated multi-arm polyethylene glycol and a diluent; the second component is an accelerator solution; the second component and the first component are mixed and then cross-linked in situ to form the long-acting developing medical hydrogel, so that the defect that conventional hydrogel cannot realize CT developing imaging in vivo for a long time is overcome, and the strength of the hydrogel can be adjusted by adjusting the arm number of amine salt of a multi-arm developing agent under the condition that the concentration is not changed; meanwhile, after the multi-arm developing agent amine salt and the succinimide ester end-capped multi-arm polyethylene glycol are mixed, the effective use time of the succinimide ester end-capped multi-arm polyethylene glycol can be prolonged, sufficient operation time is provided for surgical operation of clinicians, and in addition, the multi-arm developing agent amine salt can tolerate conventional Co60 irradiation sterilization.

The invention relates to the technical field of pet supplies, in particular to coal gangue cat litter and a preparation method thereof. The coal gangue cat litter comprises the following components: activated coal gangue and macromolecular organic matters; the activated coal gangue is obtained by activating an oxidizing agent; the macromolecular organic matter is selected from at least one of polyacrylamide, polyethylene glycol, maleimide, suberic acid disuccinimide ester, carbodiimide, agar, xanthan gum, gelatin, pectin, Arabic gum and guar gum. The invention also provides a preparation method of the coal gangue cat litter, which comprises the following steps: crushing the coal gangue into powder, adding water and an oxidizing agent, adding the macromolecular organic matter and the acid into the obtained activated coal gangue to obtain micelles, and granulating to obtain the coal gangue cat litter. The defects that existing cat litter is insufficient in strength and prone to forming dust due to mutual friction are overcome, and the problems that existing cat litter products lack timely feedback on health conditions of urinary and digestive systems of pet cats, and the existing cat litter products are high in raw material cost and prone to generating peculiar smells are solved.

The invention discloses a supramolecular polymer based on lipoic acid-based polydimethylsiloxane as well as an application and a degradation method of the supramolecular polymer. The supramolecular polymer is prepared by synthesizing an intermediate from lipoic acid and derivatives thereof and N, N '-succinimido carbonate, and then reacting with polydimethylsiloxane with different chain lengths and viscosities. The synthesis is simple, the raw material cost is low, and industrial production is easy. The polymer can be processed into different structures, is used as a dielectric layer of a capacitive sensor, and has good performance. Besides, the polymer can be degraded in a chloroform solution containing benzyl mercaptan and 1, 8-diazabicyclo [5.4. 0] undec-7-ene, and can be completely dissolved within 48 hours, so that the problems of complex synthesis, high cost and non-degradability of the existing material are solved.

The application relates to a preparation and application method of a novel crosslinking agent enrichment based on a sulfur-fluorine exchange reaction (SuFEx), and belongs to the technical field of organic synthesis. The application is based on the existing trimethylpiperidyl bis-succinimidyl ester crosslinking agent, and a series of crosslinking agents with a single end or a double end of benzylsulfonyl fluoride are synthesized based on the SuFEx reaction. The crosslinking agent has the characteristics of enrichment, adjustable arm length, multiple reaction sites, isotope quantification and the like, not only improves the crosslinking depth and coverage, but also can realize the relative quantification of proteins. The application provides important technical support for in-situ protein analysis based on a chemical crosslinking strategy.

The invention relates to a preparation method of coupling magnetic beads for microbial collection, which adopts SPDP (N-succinimido-3-(2-pyridine dithio) propionate) as a cross-linking agent to realize directional fixation of recombinant protein A-Cys on magnetosome, and then the recombinant protein A-Cys can be coupled with an antibody to form the coupling magnetic beads for microbial collection with specific antigen binding. The coupling magnetic bead for microbial collection prepared by the preparation method provided by the invention can be efficiently coupled with different types of antibodies, and has a relatively wide application prospect.

The application discloses an alkaline phosphatase antibody label and a preparation method, a detection reagent and a kit thereof, relates to the field of protein coupling technology. The alkaline phosphatase antibody label provided by the technical scheme of the application is prepared by activating alkaline phosphatase by a polyethylene glycol compound containing a succinimidyl ester group and SMCC, or is prepared by modifying alkaline phosphatase by a polyethylene glycol molecule with an amino group and then activating by SMCC. Compared with the traditional Traut's-SMCC method, the alkaline phosphatase antibody label has improved hydrophilicity, improved solubility, and certain steric hindrance, so that the possibility of continued reaction of the excess active sites on the alkaline phosphatase can be reduced, the strength of the cross-linking and aggregation of the alkaline phosphatase and the antibody can be reduced without affecting the cross-linking efficiency, so that the stability and yield of the alkaline phosphatase antibody label are improved, and batch differences are reduced.

The invention relates to the technical field of high-performance polyester, and discloses a high-toughness antibacterial DTY polyester yarn and a preparation method and application thereof. The preparation method comprises the following steps: adding chitosan into an acetic acid aqueous solution, uniformly stirring, adding protocatechuic acid and carboxylated modified graphene, uniformly stirring, adding N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride, stirring and reacting at room temperature under the protection of nitrogen, removing a solvent, washing and drying to obtain modified chitosan; adding the modified chitosan into tetrahydrofuran, uniformly stirring, adding 4-benzoyl chloride isocyanate and triethylamine, stirring and reacting at room temperature under the protection of nitrogen, removing a solvent, washing and drying to obtain an antibacterial agent; the preparation method comprises the following steps: adding an antibacterial agent into a solvent, uniformly stirring to obtain an antibacterial finishing agent, adding the surface modified DTY polyester yarn, dipping, taking out, washing and drying to obtain the high-toughness antibacterial DTY polyester yarn, and the polyester yarn is good in antibacterial property and washing resistance and can be applied to the field of textiles and garments.

This invention relates to flake silver pigments having a dm in the range of 2.0 to 100.0 µm. 50 With an aspect ratio greater than 25, at least partially coated with an additive component, wherein the additive component is: I) A thiol represented by general formula (Ia): A1-S-A2 or a dithiol represented by general formula (Ib): A1-S-S-A2, wherein A1 and A2 represent alkyl groups having 1 to 8 carbon atoms and A1=A2, or A1 and A2 independently represent residues containing polar or polarizable functional groups selected from the group consisting of esters, carboxyl groups, amines, hydroxyl groups, benzyl groups, benzoic acid groups, furanyl groups, furfuryl groups, pyrroleyl groups, pyridyl groups, pyrazolyl groups, pyrazolyl groups, oxazolyl groups, isoxazolyl groups, ethers, and combinations thereof, wherein these polar or polarizable functional groups are separated from the sulfur or disulfide atom by a maximum of 3 non-aromatic carbon atoms, and wherein the maximum total length of the thiol or dithiol involves a chain containing a maximum of 8 non-aromatic carbon atoms, and the maximum length difference between A1 and A2 is within 2 atoms of each other, or (II) From general formula (IIa): HS-(CH(A3)) x -(R) 1 ) y -A3 represents or is derived from general formula (IIb): HS-R 4 -CO-O-R 5 The thiol represented by R 1 It is an alkylene or phenylene having 1 to 6 carbon atoms, wherein A3 is independently selected from NR. 2 2. OH, COOH, COOR 3 The group consisting of N-succinimide and 1-amino-2-carboxyethyl, wherein integers x and y are 0 or 1, and x + y = 1 or 2, and wherein R 2 Independently selected from the group consisting of H, methyl, ethyl, and propyl, and R among them. 3 Select from the group consisting of H, methyl, ethyl or propyl, butyl, pentyl, hexyl, N-succinimide and 2-aminoacetyl acid, and R among them. 4 It is methylene or ethylene, and R in it 5 The additive component is selected from the group consisting of alkyl groups having 1 to 6 C atoms and N-succinimides, wherein the maximum total length of the thiol of formula (IIa) or (IIb) involves a chain containing up to 8 non-aromatic C atoms, or (III) is an amine having ether functionality and not more than 8 non-aromatic C atoms, and wherein the additive component can be one molecule or a mixture of two or more of any molecule represented by any one of formulas (Ia), (Ib), (IIa), (IIb) and (III).

The invention discloses a preparation method of a silicon-based antibacterial agent and application of the silicon-based antibacterial agent in preparation of a thin film, and the preparation method comprises the following steps: adding mesoporous silica and 3-aminopropyltriethoxysilane into a container filled with dry toluene, carrying out reflux stirring at 90 DEG C for 24 h, and cooling to room temperature after the reaction is complete, so as to obtain the silicon-based antibacterial agent. Washing with dry toluene, and carrying out vacuum drying to obtain aminated mesoporous silica; the preparation method comprises the following steps: mixing caffeic acid, 1-ethyl-(3-dimethylaminopropyl) carbodiimide, N-hydroxysuccinimide and deionized water, and ultrasonically activating carboxyl at room temperature; after ultrasonic treatment is finished, adding mesoporous silica, stirring at room temperature, centrifugally washing with absolute ethyl alcohol, and drying in vacuum to obtain a light brown powdery product, namely the silicon-based antibacterial agent. The thermal degradation rate of the ASBACA / PVA antibacterial composite film in the degradation stage is lower than that of a pure PVA film, and the thermal degradation temperature of the ASBACA / PVA antibacterial composite film in the degradation stage is higher than that of the pure PVA film, so that the thermal stability of the PVA film is possibly enhanced through the interaction between PVA and the antibacterial agent ASBACA.

This invention discloses an Angiopep-2 modified fluorescent polyamide-amine dendritic polymer and its preparation method, as well as a method for preparing nanoparticles based thereon, belonging to the field of nanomaterials technology. The preparation method includes the following steps: mixing the polyamide-amine dendritic polymer with a first phosphate buffer to obtain solution I; mixing maleimide-polyethylene glycol-N-hydroxysuccinimide with the first phosphate buffer to obtain solution II; mixing solution I and solution II and reacting them, followed by ultrafiltration purification to obtain intermediate A; dissolving Angiopep-2 in a second phosphate buffer and reacting it with intermediate A, followed by freeze-drying to obtain intermediate B; dissolving intermediate B in a dimethyl sulfoxide solution to obtain solution III; dissolving fluorescein isothiocyanate in a dimethyl sulfoxide solution to obtain solution IV; mixing solutions III and IV and reacting them under a protective gas atmosphere in the dark; freeze-drying to obtain the Angiopep-2 modified fluorescent polyamide-amine dendritic polymer.

The application discloses a microfluidic chip for extracellular vesicle capture and detection, and belongs to the technical field of microfluidic chips.The microfluidic chip comprises a polydimethylsiloxane layer and a glass substrate layer; the polydimethylsiloxane layer comprises microfluidic channel units arranged in a matrix, and the microfluidic channel units are sequentially connected by an outlet channel, a fishbone array structure and an inlet channel; and the surface of the glass substrate layer is modified with biotin-polyethylene glycol-N-hydroxysuccinimidyl ester and N-hydroxysuccinimidyl ester-polyethylene glycol.The microfluidic chip disclosed by the application can specifically capture target substances and improve the capture efficiency of the target substances.

The invention relates to the field of biological materials, and provides an anti-freezing hemostatic gel material and a preparation method thereof, and the preparation method comprises the following steps: S1, preparing an EA compound; the preparation method comprises the following steps: carrying out a reaction on epigallocatechin gallate and 3-acrylamide phenylboronic acid to prepare an EA compound; s2, preparing a gel precursor liquid: heating methylacryloylated gelatin and acrylic acid in deionized water until the methylacryloylated gelatin and the acrylic acid are completely dissolved; adding the anti-freezing compound and uniformly mixing; then, acrylic acid N-succinimide ester is added, and the gel precursor liquid is obtained; the anti-freezing compound is prepared by mixing nano cellulose, proline and glycerol; s3, preparing the anti-freezing hemostatic gel material: mixing the EA compound, the gel precursor fluid, acrylic acid, an initiator and an accelerant for reaction to prepare the anti-freezing hemostatic gel material. The anti-freezing hemostatic gel material disclosed by the invention is good in adhesiveness and high in mechanical strength, and can well meet the use requirements of a plateau environment.

The application relates to the technical field of biological medicine, in particular to a radioactive embolism gel, a preparation method thereof and a radioactive embolism agent. The radioactive embolism gel comprises the following components: a radionuclide; a skeleton material comprising polyethylene glycol dimercapto; a chelating ligand comprising 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetracarboxylic acid-N-hydroxysuccinimidyl ester; wherein the chelating ligand is covalently grafted on the skeleton material, and the radionuclide is combined with the chelating ligand through chelation. The radioactive embolism gel has the characteristics of high radiochemical purity and good stability, can be made into a gel embolism agent integrating arterial tumor embolism and local radiotherapy, realizes the synergistic treatment of vascular embolism and internal irradiation, and has a good clinical application prospect.

The invention discloses a photocuring biological tissue adhesive as well as a preparation method and application thereof, and relates to the technical field of biomedical materials. The photocuring biological tissue adhesive comprises: (1) a photocuring cross-linked network forming unit, which comprises: (1) a methylacryloylated natural polymer with amino on the main chain; (2) F127DA; (2) a dynamic and stable chemical crosslinking unit comprising: (1) an oxidized polysaccharide comprising at least one of oxidized dextran, oxidized hyaluronic acid, and oxidized cellulose; (2) succinimide acrylate; (3) a photoinitiator comprising: a lithium phenyl (2, 4, 6-trimethylbenzoyl) phosphate salt or a photoinitiator composition, the photoinitiator composition comprising: eosin Y at a concentration of 0.05 to 0.25 mmol / L; triethanolamine, the mass volume ratio of which is 1.5% to 3%; and N-vinylcaprolactam in a mass volume ratio of 1% to 2%. According to the invention, the visual pathway is not obstructed while firm bonding is realized, and the application prospect of the high-precision advanced field is expanded.

The invention discloses a preparation method of semaglutide and application of the semaglutide in treatment of diabetes mellitus, and relates to the technical field of medicine preparation, and the preparation method comprises the following steps: preparing intermediates SEM120, SEM110 and SEM130, and carrying out condensation and deprotection reaction to obtain a semaglutide crude product; the preparation method comprises the following steps: reacting a semaglutide crude product with fluorenylmethoxycarbonyl succinimide ester, and carrying out N-terminal specific protection to obtain an Fmoc-semaglutide intermediate; reacting the Fmoc-semaglutide intermediate with a succinic anhydride derivative, and carrying out hydrophobic modification; reacting the product subjected to hydrophobic modification with a piperidine solution, performing deprotection, and purifying to obtain modified semaglutide; by adopting the specific raw and auxiliary material ratio and reaction conditions, the method has the advantages of simple process, high yield, good product purity and the like, and is suitable for industrial production.

The invention relates to the technical field of biomedical materials, and particularly discloses a preparation method of hydrogel formed by combining terminal modification of PluronicF127 with HA and CuS-NDs, anhydrous tetrahydrofuran is taken and placed in a container, and then PluronicF127, N, N '-disuccinimido carbonate and 4-dimethylaminopyridine are taken and stirred and mixed together; adding anhydrous dimethyl sulfoxide, stirring and mixing, adding beta-alanine into ultrapure water, adjusting the pH value, and stirring and dissolving; adding the mixture into the mixed solution for stirring reaction; the reaction liquid is placed in a dialysis bag for dialysis, and modified PluronicF127 is obtained; cuCl2. 2H2O and sodium citrate are taken and added into ultrapure water, and after the pH is adjusted, stirring is carried out for dissolution; adding sodium sulfide nonahydrate into ultrapure water, stirring and dissolving; adding the solution, stirring and mixing to obtain a CuS-NDs solution; the CuS-NDs solution is subjected to freeze drying, and freeze-dried CuS-NDs powder is obtained; and mixing and stirring the PluronicCF127 solution, the hyaluronic acid solution and the CuS-NDs solution in an ice-water bath to obtain a hydrogel finished product. The degradable plastic has the characteristics of good mechanical strength and viscosity, and excellent biodegradability, biology and safety.