Preparation method and application of magnetic nanoparticles crossing blood-eye barrier

A magnetic nanoparticle and barrier technology, which is applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, and pharmaceutical formulas, etc., can solve the problem of restricting the application of nanomaterials in the treatment of eye diseases and hindering the entry of drugs into the eyes and other issues to achieve the effect of expanding the application potential

Active Publication Date: 2018-06-08
WENZHOU MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the blood-ocular barrier also prevents most drugs from entering the eye
At present, there are no relevant reports and applications of BMs crossing the blood-ocular barrier, which limits the application of nanomaterials based on BMs in the treatment of eye diseases, so it is necessary to study and improve this

Method used

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  • Preparation method and application of magnetic nanoparticles crossing blood-eye barrier
  • Preparation method and application of magnetic nanoparticles crossing blood-eye barrier
  • Preparation method and application of magnetic nanoparticles crossing blood-eye barrier

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Embodiment

[0029] (1) Firstly, bacterial magnetosome BMs were isolated and purified from Magnetospira MSR-1. The specific method is as follows: collect the MSR-1 strain in the logarithmic growth phase, and resuspend the strain in PBS buffer. The crudely extracted BMs were collected by sonication and magnetic recovery. The crudely extracted BMs were further purified by ultrasonic cleaning and magnetic recovery until no protein and DNA residues could be detected in the supernatant, and purified BMs were obtained.

[0030] (2) Using SPDP (N-succinimide 3-(2-pyridyldithio) propionate) cross-linking agent, the amino terminal of BMs was combined with cysteine ​​in TAT polypeptide (NH2-CGRKKRRQRRRK-COOH) The combination of sulfhydryl group and TAT-BMs carrier can cross the blood-ocular barrier. The specific method is as follows: BMs and SPDP were mixed at room temperature for 2 hours, the activated BMs-SPDP and TAT polypeptide were mixed at 4°C overnight, and the mixture was washed repeatedly...

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Abstract

The invention discloses a preparation method and application of magnetic nanoparticles crossing a blood-eye barrier. The preparation method comprises taking purified bacterial magnetosomes BMs, and binding the amino terminals of the bacterial magnetosomes BMs and cysteine in a TAT polypeptide through an N-succinimido-3-(2-pyridinedithio)propionate as a cross-linking agent to obtain magnetic nanoparticles crossing a blood-eye barrier. The magnetic nanoparticles are TAT-BMs composite carriers. The magnetic nanoparticles can cross a blood-eye barrier and expand the application potential of magnetic nanomaterials in treatment on eye diseases.

Description

technical field [0001] The invention relates to a magnetic nano particle, in particular to a preparation method and application of the magnetic nano particle crossing the blood-eye barrier. Background technique [0002] Bacterial magnetosomes (BMs) are biosynthesized magnetic nanoparticles with a unique nanostructure, covered with a biofilm similar to the cell membrane structure, and stable in size and shape. BMs have a wide range of potential applications in the field of biomedicine, such as nuclear magnetic resonance imaging, drug loading, and magnetic hyperthermia. Different from chemically synthesized magnetic nanoparticles, BMs are synthesized intracellularly by magnetotactic bacteria, and the main component is Fe 3 o 4 or Fe 3 S 4 , the size is 30-120 nm. [0003] The eye is a very fragile organ of the human body, and the entire organ forms an almost airtight space under the protection of the blood-ocular barrier. However, the blood-ocular barrier also prevents m...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/46A61K47/42A61K31/704A61P27/02A61P35/00
CPCA61K9/5169A61K9/5176A61K31/704A61K47/42A61K47/46
Inventor 齐蕾吕秀娟杨梅潘统鹤程如梅姚清清张立树陈路呀赵长荣戴黎明
Owner WENZHOU MEDICAL UNIV
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