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Urine microalbumin detection reagent strip and preparation method thereof

A technology of urine microalbumin and detection reagent, applied in the field of medical examination, can solve the problems of low sensitivity and the like, and achieve the effects of good stability, easy visual discrimination and machine reading, and accurate measurement results

Active Publication Date: 2018-06-29
李宏奎
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the detection principle of the immunoturbidimetric method is to react to generate an insoluble antigen-antibody complex and generate a certain turbidity, the level of turbidity reflects the content of microalbumin in the sample, resulting in the existing urinary microalbumin kits Inevitable lack of low sensitivity

Method used

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  • Urine microalbumin detection reagent strip and preparation method thereof
  • Urine microalbumin detection reagent strip and preparation method thereof
  • Urine microalbumin detection reagent strip and preparation method thereof

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Embodiment

[0016] A reagent strip for detecting urine microalbumin, comprising a support plate and a filter paper sheet, the filter paper sheet is attached to the support plate, and a dye solution is immersed in the filter paper sheet, and the dye solution is composed of reagent I and reagent II , the reagent I consists of 0.10mmol / L cyanine dye, pH7.4, 20mmol / L triethanolamine buffer, 0.01g / L Triton X-100, 0.10g / L sodium benzoate, 0.10g / L PEG -4000; Reagent II consists of pH7.4.0, 50mmol / L disodium hydrogen phosphate-sodium dihydrogen phosphate buffer, 0.01mmol / L O-(N-succinimidyl)-N N N'N'-tetramethyl Urea tetrafluoroborate, sodium azide of 0.10g / L, dimethylacetamide of 1g / L; The cyanine dye is a compound of formula I,

[0017]

[0018] The preparation method of the above urine microalbumin detection reagent strip comprises the following steps:

[0019] (1) Weigh the raw materials cyanine dye, triethanolamine, Triton X-100, sodium benzoate, PEG-4000, add purified water to make up t...

Embodiment 2

[0025] A reagent strip for detecting urine microalbumin, comprising a support plate and a filter paper sheet, the filter paper sheet is attached to the support plate, and a dye solution is immersed in the filter paper sheet, and the dye solution is composed of reagent I and reagent II The reagent I consists of 10mmol / L cyanine dye, pH8.0, 100mmol / L triethanolamine buffer, 0.1g / L Triton X-100, 1g / L sodium benzoate, and 2g / L PEG-4000; The composition of reagent II is pH8.0, 100mmol / L disodium hydrogen phosphate-sodium dihydrogen phosphate buffer, 1mmol / LO-(N-succinimidyl)-N N N'N'-tetramethyl tetrafluoroborate urea , the sodium azide of 2g / L, the dimethylacetamide of 10g / L; Described cyanine dye is the compound of formula I,

[0026]

[0027] The preparation method of the above urine microalbumin detection reagent strip comprises the following steps:

[0028] (1) Weigh the raw materials cyanine dye, triethanolamine, Triton X-100, sodium benzoate, PEG-4000, add purified water...

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Abstract

The invention provides a urine microalbumin detection reagent strip and a preparation method thereof, and belongs to the technical field of medical examination. The reagent strip comprises a supporting plate and a filter paper sheet, wherein the filter paper sheet is attached onto the supporting plate; the filter paper sheet is immerged in dyeing liquid; the dyeing liquid is prepared from a reagent I and a reagent II; the reagent I is prepared from cyanine dyes, trolamine buffer solution, Triton X-100, sodium benzoate and PEG-4000; the reagent II is prepared from disodium hydrogen phosphate-monosodium orthophosphate buffer solution, O-(N-succinimido)-N N N'N'-tetramethyl tetrafluorohydrazine carbamide borate, sodium azide and dimethyl acetamide. The cyanine dyes of the prepared urine microalbumin detection reagent strip can fast react with microalbumin in urine; when the concentration of the microalbumin is in a range of 0 to 200mg / L, the test paper is at the obvious color gradation from being achromatic color to dark blue; the visual distinguishing and machine reading are easy; the determination result is accurate; the stability is high.

Description

technical field [0001] The invention belongs to the technical field of medical testing, and in particular relates to a urine microalbumin detection reagent strip and a preparation method thereof. Background technique [0002] Albumin is one of the important plasma proteins. Under normal circumstances, albumin has a large molecular weight and cannot cross the glomerular basement membrane. Therefore, the urine of healthy people only contains a very low concentration of albumin, specifically per liter Urine albumin does not exceed 20mg, so it is also called "urine microalbumin test". In disease, the glomerular basement membrane is damaged and the permeability changes. Any lesion that can cause the glomerular basement membrane permeability to increase can lead to the excretion of albumin. At this time, albumin can enter the urine, and the concentration of urinary albumin can continue to rise. The most ideal way to measure urine albumin is to take a 24-hour urine sample. Since ...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/7759
Inventor 李宏奎
Owner 李宏奎
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