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Preparation method of fluorogold nanocomposite used for cell imaging

A fluorescent gold nanometer and cell imaging technology, which is applied in the field of preparation of fluorescent gold nanocomposites, can solve the problems of easy photobleaching, limit the application of fluorescence methods, and low brightness, and achieve broad application prospects, increase fluorescence intensity, and low cost. Effect

Inactive Publication Date: 2016-07-27
UNIV OF SCI & TECH BEIJING
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Problems solved by technology

[0002] Fluorescent labeling has become an important means of chemical and biological sensing and imaging research, but traditional organic dyes have disadvantages such as low brightness and easy photobleaching, which greatly limit the application of fluorescence methods in the field of biological imaging

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  • Preparation method of fluorogold nanocomposite used for cell imaging

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preparation example Construction

[0021] An embodiment of the present invention is a method for preparing a fluorescent gold nanocomposite for cell imaging, comprising:

[0022] The step of synthesizing gold nanoparticles; the step of coating the polylysine shell on the surface of the gold nanoparticles; and using the reaction between the amino group and the succinimide group on the surface of the shell to modify the interaction between the fluorescent dye and the targeting molecule step.

[0023] Preferably, the step of synthesizing gold nanoparticles is: soak the flask with aqua regia for 30 minutes, and clean it with deionized water; add deionized water and amino acid respectively in the ratio of adding 5 mg of amino acid to every 10 mL of deionized water in the flask, at room temperature Stir fully to dissolve; then add chloroauric acid solution in a ratio of 100 μL for every 5 mg of amino acid, the concentration of the chloroauric acid solution is 0.1mol / L, stir at room temperature for 3 hours, and the so...

Embodiment 1

[0034] A method for preparing a fluorescent gold nanocomposite for cell imaging, comprising the following steps:

[0035] Step 1. Synthesis of gold nanoparticles

[0036] Take a 25mL round-bottom flask, soak it in aqua regia for 30 minutes, and then clean it with deionized water. Add 10 mL of deionized water and 5 mg of tryptophan into the flask, stir well at room temperature to dissolve. Then add 100 μL chloroauric acid solution (0.1mol / L concentration), stir at room temperature for 3 hours, the solution gradually changes from colorless to dark red;

[0037] Step 2, coating of polylysine shell

[0038]Dilute 2 mL of the gold nanoparticle solution prepared in step 1 to 4 mL and add it to a 10 mL round bottom flask. Add 100 μL of polylysine solution (concentration: 25 mg / mL) and 0.2 mg of disuccinimide suberate to it, adjust pH=9, and stir at room temperature for 3 hours;

[0039] Step 3. Modification of fluorescent dyes and targeting molecules

[0040] Centrifuge the coat...

Embodiment 2

[0042] A method for preparing a fluorescent gold nanocomposite for cell imaging, comprising the following steps:

[0043] Step 1. Synthesis of gold nanoparticles

[0044] Take a 25mL round-bottomed flask, soak it in aqua regia for 30 minutes and then clean it with deionized water; add 10mL deionized water and 5mg tryptophan to the flask, and stir well at room temperature to dissolve. Then add 100 μL chloroauric acid solution (0.1mol / L), stir at room temperature for 3 hours, the solution gradually changes from colorless to dark red;

[0045] Step 2, coating of polylysine shell

[0046] Dilute 2 mL of the gold nanoparticle solution prepared in step 1 to 4 mL and add it to a 10 mL round bottom flask. Add 100 μL of polylysine solution (25 mg / mL) and 0.2 mg of 3,3’-dithiobis(sulfosuccinimidyl propionate) to it, adjust the pH to 9, and stir at room temperature for 3 hours;

[0047] Step 3. Modification of fluorescent dyes and targeting molecules

[0048] Centrifuge the coated pr...

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Abstract

The invention relates to the technical field of preparation of composite nanoparticles and provides a preparation method of a fluorogold nanocomposite used for cell imaging. The preparation method comprises the following steps of: synthesizing gold nanoparticles; coating a polylysine shell layer onto the surfaces of the gold nanoparticles; and modifying fluorescent dye and a targeting molecule through reaction between an amino group on the surface of the shell layer and a succinimido group. The preparation method provided by the invention has the beneficial effects that electrostatic self-assembly and an amino crosslinking method are adopted for preparation, operation is simple, and cost is low; amino acid, polylysine and polypeptide molecules are biomolecules respectively and are environment-friendly, usage of a reducing agent and a surfactant with high toxicity is avoided, and biocompatibility is good; the fluorescence intensity of dye molecules and photostability in cells are improved by utilizing surface plasma effect of the gold nanoparticles; introduction of the targeting molecule improves fluorescent imaging effect of composite on specific tumour cells; the fluorogold nanocomposite has a broad application prospect in the fields of cell imaging, disease detection, research and development of targeted medicines and the like.

Description

technical field [0001] The invention relates to the technical field of preparation of composite nanoparticles, in particular to a method for preparing a fluorescent gold nanocomposite for cell imaging. Background technique [0002] Fluorescent labeling has become an important means of chemical and biological sensing and imaging research, but traditional organic dyes have disadvantages such as low brightness and easy photobleaching, which greatly limit the application of fluorescence methods in the field of biological imaging. In recent years, with the development of nanotechnology, fluorescent nanoparticles have been increasingly used in sensing and imaging. Different from traditional organic small molecule dyes, fluorescent nanostructures such as semiconductor quantum dots, conjugated polymers, carbon nanodots, and dye-modified nanoparticles have the advantages of high brightness and good photostability in cells. It has been reported that the fluorescence imaging of tumor ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/06
CPCC09K11/025C09K11/06C09K2211/1029
Inventor 李立东唐馥王淳王晓瑜
Owner UNIV OF SCI & TECH BEIJING
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