Keratin nanoparticle and preparation method thereof

A keratin and nanoparticle technology, applied in the field of keratin nanoparticle and its preparation, can solve the problems of poor biocompatibility, inability to absorb and degrade, shorten the hemostasis time, reduce blood loss, improve in vitro coagulation and The effect of hemostatic effect in vivo

Active Publication Date: 2016-02-24
JIANGSU BANGZE BIOLOGICAL MEDICINE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are many materials for hemostasis on the market, such as mineral material zeolite powder, although it has a good hemostatic effect, it cannot be absorbed and degraded, and can only be used for emergency hemostasis of trauma; and some synthetic hemostatic materials are biocompatible Poor performance, etc. have certain limitations in use

Method used

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  • Keratin nanoparticle and preparation method thereof
  • Keratin nanoparticle and preparation method thereof
  • Keratin nanoparticle and preparation method thereof

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preparation example Construction

[0025] (3) Preparation of nanosuspension: Inject keratin aqueous solution into 20-100mL of the prepared dispersion solution through a constant flow pump at a constant rate of 0.05-0.2mL / min, and finally the keratin in the nanosuspension The protein content is 1mg / mL. At the same time as instillation, the dispersion solution is ultrasonically dispersed with a power of 300-400W in an ice bath;

[0026] (4) Centrifuge the suspension obtained in step (3) at a high speed, the centrifugal speed is 10000-12000r / m, the centrifugation time is 20-30min, and the centrifugation temperature is 4°C. After freezing for 12 hours, freeze-drying for 24 hours, drying the resulting powder agglomerates and grinding to obtain keratin nanoparticles.

[0027] The role of the dispersion solution in step (1) is to precipitate the keratin in the acidic solvent, and the pH of the dispersion solution cannot be too high, otherwise the keratin cannot be completely precipitated in the precipitant; meanwhile,...

Embodiment 1

[0031] A preparation method of keratin nanoparticles:

[0032] (1) Take glacial acetic acid in a beaker, add deionized water to dilute to pH 2.0, and prepare a dispersion solution for subsequent use;

[0033] (2) Weigh 75 mg of keratin extract, dissolve it in 15 mL of deionized water under magnetic stirring, and prepare an aqueous keratin solution with a mass concentration of 0.5%;

[0034] (3) Get 20 mL of the dispersion solution made in step (1) in a 25 mL beaker, and disperse ultrasonically with a power of 300 W under ice bath conditions. At the same time, get 5 mL of the keratin solution made in step (2). The flow pump was injected into the dispersion solution at a rate of 0.05mL / min. After the keratin solution was injected, the ultrasound was stopped to obtain a keratin nanosuspension. Repeat 3 times under the same conditions;

[0035] (4) Centrifuge the above nanosuspension at 10,000 rpm for 30 min at 4°C, pour the centrifuged supernatant, and put the centrifuged sedime...

Embodiment 2

[0037] A preparation method of keratin nanoparticles:

[0038] (1) Take hydrochloric acid in a beaker, add deionized water to dilute to pH 1.0, and prepare a dispersion solution for subsequent use;

[0039] (2) Weigh 187.5 mg of keratin extract, dissolve it in 25 mL of deionized water under magnetic stirring, and prepare an aqueous keratin solution with a mass concentration of 0.75%;

[0040](3) Get 40mL of the dispersion solution prepared in step (1) in a 50mL beaker, and disperse ultrasonically with a power of 400W under ice bath conditions. The flow pump was injected into the dispersion solution at a rate of 0.1mL / min. After the keratin solution was injected, the ultrasound was stopped to obtain a keratin nanosuspension. Repeat 3 times under the same conditions;

[0041] (4) Centrifuge the above nanosuspension at 11000rpm for 25min at 4°C, pour the centrifuged supernatant, and put the centrifuged sediment in a glass disc, pre-freeze at -40°C for 12 hours, then freeze-dry f...

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Abstract

The invention discloses a keratin nanoparticle and a preparation method thereof. The average particle size of the keratin nanoparticle ranges from 105 nm to 150 nm. The keratin nanoparticle is prepared in the following steps that firstly, a dispersing solution is prepared, wherein a dilute acetic acid solution with the pH being 2-3 or a diluted hydrochloric acid solution with the pH being 1-3 is prepared for use; secondly, a keratin aqueous solution with the mass concentration being 0.5-1.25% is prepared, wherein deionized water is added into a keratin extract, stirred and dissolved; thirdly, a nano suspension is prepared, wherein the keratin aqueous solution is added into the prepared dispersing solution of a preset amount in a dropwise mode, and the dispersing solution is subjected to ultrasonic dispersing while dropwise addition is conducted to obtain a keratin nano suspension; fourthly, the suspension obtained in the third step is subjected to high-speed centrifugation, after centrifugation, centrifugal supernate is poured, and powder obtained after centrifugal settlement and drying blocks and is ground to obtain the keratin nanoparticle. The method is easy to implement, and the prepared keratin nanoparticle is uniform in appearance, can be used for medicine carriers or hemostatic materials and has good in-vitro coagulation and in-vivo hemostasis effects.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a keratin nanoparticle and a preparation method thereof. Background technique [0002] In recent years, with the continuous advancement of science and technology and the continuous development of the medical industry, people have a new understanding of hemostatic methods and materials. At present, there are many materials for hemostasis on the market, such as mineral material zeolite powder, although it has a good hemostatic effect, it cannot be absorbed and degraded, so it can only be used for emergency hemostasis of trauma; and some synthetic hemostatic materials are biocompatible Poor performance, etc. have certain limitations in use. [0003] Xueyu charcoal has been recorded in the "Compendium of Materia Medica". In ancient times, people used it for wound bleeding, which has the effects of stopping bleeding, eliminating blood stasis, diuresis and promoting mu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61K9/14A61K47/42A61P7/04
CPCA61K9/14A61K38/1748A61K47/42
Inventor 王伯初郝石磊王驹罗甜甜祝连彩赵志斌秦松柏王丹丹
Owner JIANGSU BANGZE BIOLOGICAL MEDICINE TECH CO LTD
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