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Fibrous internal and external mineralized collagen scaffold and preparation method thereof

A mineralized collagen and fiber technology, applied in the preparation and application of bone tissue defect repair materials, bionic mineralization process, synergistically mineralized collagen scaffolds inside and outside fibers and its preparation, can solve the problem of unsystematic research on degradation products, biological Compatibility needs to be studied and other issues to achieve good biological compatibility and low price

Active Publication Date: 2016-03-23
博纳格科技(天津)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the polymers used are chemically synthesized polyanion materials, and the biocompatibility after being applied to the body needs to be studied. Secondly, whether its degradation products have an impact on the body has not yet been systematically studied. Therefore, looking for a natural Stable materials with good biocompatibility are necessary

Method used

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  • Fibrous internal and external mineralized collagen scaffold and preparation method thereof
  • Fibrous internal and external mineralized collagen scaffold and preparation method thereof
  • Fibrous internal and external mineralized collagen scaffold and preparation method thereof

Examples

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Embodiment 1

[0043] The preparation of the synergistically mineralized collagen scaffold inside and outside the fiber of the present invention comprises the following steps,

[0044] (1) Extraction and self-assembly of type Ⅰ collagen

[0045]Soak the tail of fresh 8-week-old SD rats in 75% ethanol solution for 15 minutes, remove the skin of the tail and extract the tendons of the tail. All operations were performed in a deionized water tank to prevent collagen denaturation. The extracted rat tail tendon was placed in Tris-HCl-NaCl buffer solution (0.05mol / L, pH=7.4), and soaked at 4°C for 24 hours. After the rat tail tendon was taken out, it was rinsed with deionized water, placed in 0.3 M acetic acid solution, and kept stirring at room temperature for 3 days to obtain a collagen acetic acid solution. After the solution was centrifuged at 4° C. and 3000 r / min for 30 minutes, the supernatant was taken and put into a dialysis bag. Place the dialysis bag in 0.02M dipotassium hydrogen phosp...

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Abstract

The invention provides a fibrous internal and external mineralized collagen scaffold and a preparation method thereof. The fibrous internal and external mineralized collagen scaffold material not only is approximate to natural osseous tissues on chemical composition but also simulates the generation process of natural osseous tissues on formation process as far as possible, so that a characteristic and periodic (D=67 nm) transverse position structure in collagen fibers. The novel biomimetic mineralized scaffold material is remarkably superior to a scaffold material prepared according to the traditional mineralizing method in in-vivo experiments (animal experiments), in-vitro experiments (cell proliferation and osteogenic differentiation) and mechanical property experiments, thereby providing a new probability for repair of clinical osseous tissue defects in future and having a wider application prospect.

Description

technical field [0001] The invention belongs to the field of tissue engineering scaffold materials, and in particular relates to a collagen scaffold with synergistic mineralization inside and outside the fiber and a preparation method thereof, especially a bionic mineralization process, and relates to the preparation and application of bone tissue defect repair materials. Background technique [0002] Natural bone tissue is mainly a complex organic-inorganic complex formed by the orderly arrangement and combination of hydroxyapatite nanocrystals and type I collagen fibers. The repair of bone tissue defects caused by factors such as tumor resection or trauma has always been an important and difficult issue in scientific research and clinical treatment. Although autologous bone grafting technology has achieved certain results in the treatment of clinical bone tissue defects, its further development and application are restricted by the limitation of bone supply area and the se...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/20A61L27/12A61L27/58
CPCA61L27/12A61L27/20A61L27/24A61L27/58A61L2300/112A61L2300/232A61L2300/252C08L89/06C08L5/08
Inventor 张旭王瑶王浩荣李长义
Owner 博纳格科技(天津)有限公司
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