Tissue engineering material for nerve injury repair as well as preparation method and application of tissue engineering material

A technology for tissue engineering and nerve injury, applied in the field of biodegradable materials of cross-linked nerve cadherin, which can solve the problems of limited application and poor mechanical strength, and achieve the effects of linear state stability, promotion of differentiation, and promotion of recovery

Active Publication Date: 2020-05-08
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As an animal-derived extracellular matrix protein, collagen has the advantages of biocompatibility and degradability, but its mechanical strength is poor, which greatly limits its application.

Method used

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  • Tissue engineering material for nerve injury repair as well as preparation method and application of tissue engineering material
  • Tissue engineering material for nerve injury repair as well as preparation method and application of tissue engineering material
  • Tissue engineering material for nerve injury repair as well as preparation method and application of tissue engineering material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Preparation of a Linear Ordered Collagen Scaffold of Crosslinked N-cadherin (1)

[0050] The present invention provides a kind of LOCS (LOCS-Ncad) of cross-linking N-cadherin, which uses cross-linking agents Traut's and Sulfo-SMCC to cross-link N-cadherin to LOCS.

[0051] The preparation method of the LOCS of the above-mentioned cross-linked N-cadherin is as follows (the schematic diagram of the reaction principle of cross-linked N-cadherin on a linear ordered collagen scaffold is as follows: figure 1 shown in A):

[0052] (1) Use 300 μL of Traut's reagent (PBS, pH=8, 3mM EDTA) with a concentration of 5mg / mL to soak a bunch of LOCS at room temperature (the size of a bunch of LOCS is: length 4cm, diameter 4mm, and the size of each LOCS is For: length 4cm, diameter 0.25mm), immersion time is 2h; After immersion, soak and wash with PBS 3 times, each 5 minutes, obtain the LOCS modified by Traut's reagent;

[0053] (2) Use 200 μL of PBS solution (PBS, pH=7.2, 3m...

Embodiment 2

[0054] Example 2 Preparation of a Linear Ordered Collagen Scaffold of Crosslinked N-cadherin (2)

[0055] (1) Use 300 μL of Traut's reagent (PBS, pH=8, 3mM EDTA) with a concentration of 5mg / mL to soak a bunch of LOCS at room temperature (the size of a bunch of LOCS is: length 4cm, diameter 4mm, and the size of each LOCS is For: length 4cm, diameter 0.25mm), immersion time is 2h; After immersion, soak and wash with PBS 3 times, each 5 minutes, obtain the LOCS modified by Traut's reagent;

[0056] (2) Use 200 μL of PBS solution (PBS, pH=7.2, 3mM EDTA) of N-cadherin recombinant protein and Sulfo-SMCC (wherein, the final concentration of N-cadherin recombinant protein is 400 μg / mL, and the final concentration of Sulfo-SMCC is 3.2 mg / mL) at room temperature to soak the LOCS modified by Traut's reagent obtained in step (1) for 2 hours to obtain cross-linked N-cadherin LOCS.

experiment example 1

[0059] Experimental example 1 The effect of the amount of Traut's reagent and N-cadherin recombinant protein on cross-linking efficiency

[0060] According to the principle of cross-linking N-cadherin to LOCS with the cross-linking agent Traut’s reagent and Sulfo-SMCC, the cross-linking of N-cadherin was analyzed by detecting the concentration of sulfhydryl groups, as follows:

[0061] During the preparation of the linear ordered collagen scaffolds crosslinked with N-cadherin, 300 μL of Traut’s reagent (PBS, pH=8, 3 mM EDTA) with concentrations of 0, 1, 2.5, 5, 7.5, and 10 mg / mL were soaked at room temperature A bundle of LOCS (dimensions: length 4cm, diameter 4mm) was soaked for 2 hours to obtain LOCS diluted with Traut's reagent. Detect the concentration of sulfhydryl groups after treatment with different concentrations of Traut's reagent, the results are as follows figure 1 As shown in B, the results show that the sulfhydryl concentration of LOCS (Example 1 and Example 2) ...

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Abstract

The invention relates to the technical field of tissue engineering, in particular to a tissue engineering material for nerve injury repair as well as a preparation method and application of the tissueengineering material. The tissue engineering material for nerve injury repair is a linear ordered collagen stent of crosslinked N-cadherin. The tissue engineering material prepared by crosslinkingtheN-cadherin in the linear ordered collagen stent can efficiently induce neural stem cells to migrate towards an injury region, enrich neutral stem cells in the injury region, effectively inhibit deposition of inhibitors such as chondroitin sulfate proteolgycan and promote differentiation of the neutral stem cells towards neurons so as to promote recovery of electrophysiological and motion functions; and meanwhile, the N-cadherin crosslinked linear ordered collagen stent also has stable and ordered topological structures and excellent mechanical properties and thus can be used for repairing nerve injuries such as spinal cord injury.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a cross-linked nerve-cadherin biodegradable material for repairing nerve damage, a preparation method and application of the material. Background technique [0002] The spinal cord is an important part of the central nervous system and is the conduction pathway between the brain and the peripheral nervous system. Spinal cord injury will lead to the complete or partial loss of motor and sensory functions below the injury section, which greatly reduces the quality of life of the patient and brings a huge burden to the society. At present, the clinical treatment methods for spinal cord injuries mainly include drug therapy such as neuronutrients and surgical decompression, but these methods cannot regenerate the damaged spinal cord of the patient, and thus cannot improve his motor function. [0003] With the development of tissue engineering, the scaffold material combine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/50A61L27/26C12N5/0793
CPCA61L27/50A61L27/26C12N5/0619A61L2430/32C12N2533/54C08L89/00A61L27/58A61L2430/40C12N5/0068C08L89/06A61L27/227A61L27/24A61L27/54A61L2300/412C12N2533/50C12N2537/10
Inventor 戴建武刘为远赵燕南陈冰肖志峰
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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