A tissue engineering material for nerve injury repair and its preparation method and application

A technology for tissue engineering and nerve injury, applied in the field of biodegradable materials of cross-linked nerve cadherin, which can solve the problems of poor mechanical strength and limited application, and achieve the effects of linear state stability, promotion of differentiation, and promotion of recovery

Active Publication Date: 2022-02-11
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As an animal-derived extracellular matrix protein, collagen has the advantages of biocompatibility and degradability, but its mechanical strength is poor, which greatly limits its application.

Method used

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  • A tissue engineering material for nerve injury repair and its preparation method and application
  • A tissue engineering material for nerve injury repair and its preparation method and application
  • A tissue engineering material for nerve injury repair and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Preparation of a Linear Ordered Collagen Scaffold of Crosslinked N-cadherin (1)

[0050] The present invention provides a kind of LOCS (LOCS-Ncad) of cross-linking N-cadherin, which uses cross-linking agents Traut's and Sulfo-SMCC to cross-link N-cadherin to LOCS.

[0051] The preparation method of the LOCS of the above-mentioned cross-linked N-cadherin is as follows (the schematic diagram of the reaction principle of cross-linked N-cadherin on a linear ordered collagen scaffold is as follows: figure 1 shown in A):

[0052] (1) Use 300 μL of Traut's reagent (PBS, pH=8, 3mM EDTA) with a concentration of 5mg / mL to soak a bunch of LOCS at room temperature (the size of a bunch of LOCS is: length 4cm, diameter 4mm, and the size of each LOCS is For: length 4cm, diameter 0.25mm), immersion time is 2h; After immersion, soak and wash with PBS 3 times, each 5 minutes, obtain the LOCS modified by Traut's reagent;

[0053] (2) Use 200 μL of PBS solution (PBS, pH=7.2, 3m...

Embodiment 2

[0054] Example 2 Preparation of a Linear Ordered Collagen Scaffold of Crosslinked N-cadherin (2)

[0055] (1) Use 300 μL of Traut's reagent (PBS, pH=8, 3mM EDTA) with a concentration of 5mg / mL to soak a bunch of LOCS at room temperature (the size of a bunch of LOCS is: length 4cm, diameter 4mm, and the size of each LOCS is For: length 4cm, diameter 0.25mm), immersion time is 2h; After immersion, soak and wash with PBS 3 times, each 5 minutes, obtain the LOCS modified by Traut's reagent;

[0056] (2) Use 200 μL of PBS solution (PBS, pH=7.2, 3mM EDTA) of N-cadherin recombinant protein and Sulfo-SMCC (wherein, the final concentration of N-cadherin recombinant protein is 400 μg / mL, and the final concentration of Sulfo-SMCC is 3.2 mg / mL) at room temperature to soak the LOCS modified by Traut's reagent obtained in step (1) for 2 hours to obtain cross-linked N-cadherin LOCS.

experiment example 1

[0059] Experimental example 1 The effect of the amount of Traut's reagent and N-cadherin recombinant protein on cross-linking efficiency

[0060] According to the principle of cross-linking N-cadherin to LOCS with the cross-linking agent Traut’s reagent and Sulfo-SMCC, the cross-linking of N-cadherin was analyzed by detecting the concentration of sulfhydryl groups, as follows:

[0061] During the preparation of the linear ordered collagen scaffolds crosslinked with N-cadherin, 300 μL of Traut’s reagent (PBS, pH=8, 3 mM EDTA) with concentrations of 0, 1, 2.5, 5, 7.5, and 10 mg / mL were soaked at room temperature A bundle of LOCS (dimensions: length 4cm, diameter 4mm) was soaked for 2 hours to obtain LOCS diluted with Traut's reagent. Detect the concentration of sulfhydryl groups after treatment with different concentrations of Traut's reagent, the results are as follows figure 1 As shown in B, the results show that the sulfhydryl concentration of LOCS (Example 1 and Example 2) ...

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Abstract

The invention relates to the technical field of tissue engineering, in particular to a tissue engineering material for repairing nerve damage and its preparation method and application. The invention provides a tissue engineering material for repairing nerve damage, which is a linear ordered collagen scaffold of cross-linked nerve cadherin. The tissue engineering material prepared by cross-linking neural cadherin to a linear ordered collagen scaffold can efficiently induce the migration of neural stem cells to the damaged area, enrich the neural stem cells in the damaged area, and effectively inhibit the inhibitory effect of chondroitin sulfate proteoglycans, etc. The deposition of factors promotes the differentiation of neural stem cells into neurons, and then promotes the recovery of electrophysiology and motor function; at the same time, the linear ordered collagen scaffold cross-linked by N-cadherin also has a stable and ordered topology and excellent mechanical properties , Therefore, it can be used for the repair of nerve damage such as spinal cord injury.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a cross-linked nerve-cadherin biodegradable material for repairing nerve damage, a preparation method and application of the material. Background technique [0002] The spinal cord is an important part of the central nervous system and is the conduction pathway between the brain and the peripheral nervous system. Spinal cord injury will lead to the complete or partial loss of motor and sensory functions below the injury section, which greatly reduces the quality of life of the patient and brings a huge burden to the society. At present, the clinical treatment methods for spinal cord injuries mainly include drug therapy such as neuronutrients and surgical decompression, but these methods cannot regenerate the damaged spinal cord of the patient, and thus cannot improve his motor function. [0003] With the development of tissue engineering, the scaffold material combine...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/50A61L27/26C12N5/0793
CPCA61L27/50A61L27/26C12N5/0619A61L2430/32C12N2533/54C08L89/00A61L27/58A61L2430/40C12N5/0068C08L89/06A61L27/227A61L27/24A61L27/54A61L2300/412C12N2533/50C12N2537/10
Inventor 戴建武刘为远赵燕南陈冰肖志峰
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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