Method for inducing flavonoids and terpene lactones to accumulate in plant leaves by using methyl jasmonate and application

A technology of methyl jasmonate and inducing plants, applied in the directions of botanical equipment and methods, chemicals for biological control, applications, etc., can solve problems such as unrealized industrialization, and achieve the effects of low cost and high content

Active Publication Date: 2019-07-19
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regarding the synthesis of EGB, although there have been experimental studies based on cell lines, the way to obtain EGB from in vitro culture has not yet achieved industrialization

Method used

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  • Method for inducing flavonoids and terpene lactones to accumulate in plant leaves by using methyl jasmonate and application
  • Method for inducing flavonoids and terpene lactones to accumulate in plant leaves by using methyl jasmonate and application
  • Method for inducing flavonoids and terpene lactones to accumulate in plant leaves by using methyl jasmonate and application

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Embodiment Construction

[0028] 1. Materials

[0029] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified, and the reagents and other materials used are all commercially available products unless otherwise specified.

[0030] 2. Method

[0031] 2.1 Configure methyl jasmonate solution

[0032] After dissolving the solid methyl jasmonate with absolute ethanol, add Tween20, and then dilute with distilled water, wherein the final volume fraction of ethanol is 2-5%, and the final volume fraction of Tween20 is 0.02-0.05%.

[0033] Configure 0-100mg·l according to the above method -1 Methyl jasmonate solutions with different concentration gradients were used for follow-up experiments to obtain methyl jasmonate with four concentration gradients of 0mg / L (CK), 20mg / L (MJ1), 40mg / L (MJ2), and 80mg / L (MJ3). solution.

[0034] 2.2 Test treatment

[0035] The 10-year-old Dafo finger grafted ginkgo tree in Taixing City, Jiangsu Province was sel...

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Abstract

The invention discloses a method for inducing flavonoids and terpene lactones to accumulate in plant leaves by using methyl jasmonate and application, and belongs to the technical field of biology. The method comprises the following step: when plant leaf cells are completely mature, spraying a 20-80mg/L methyl jasmonate solution onto the plant leaves for 1-2 times, so that a ginkgo biloba extractwith high active ingredient content can be obtained from the treated plant leaves through a conventional method. Compared with the prior art, the method has the advantages of being simple and easy toimplement, low in cost, free of pollution, stable and efficient; the flavonoids and terpene lactones obtained after regulation and control by the method disclosed by the invention are high in content;wherein the contents of quercetin, kaempferol, isorhamnetin, total flavonoids, ginkgo terpene lactone A, ginkgo terpene lactone B and total terpene lactone are respectively increased by 29.84%, 18.08%, 35.94%, 26.24%, 30.36%, 60.65% and 35.41% compared with a control group.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method and application of using methyl jasmonate to induce the accumulation of flavonoids and terpene lactones in plant leaves. Background technique [0002] Methyl-Jasmonic acid (MeJA) is a derivative of cyclopentanone jasmonic acid. When exogenous methyl-jasmonic acid enters the plant, it will be hydrolyzed into jasmonic acid and play a role. Jasmonic acid is a cell signal transduction An important intermediate substance in the system, which can combine with various free amino acids to form an inducible jasmonic acid-amino acid complex, participate in the transduction and amplification of plant defense signals, induce the expression of plant defense genes, and the synthesis of defense proteins, etc. . In addition, exogenous methyl jasmonate can also directly act as a signal mediator in plant cells, leading to stress resistance in cells and inducing the synthesis of various secon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N37/42A01P21/00
CPCA01N37/42
Inventor 汪贵斌国颖冯宇
Owner NANJING FORESTRY UNIV
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