Method for detecting ginkgo leaf extract and diphyridamole injection preparation

The technology of an injection preparation and a detection method, which is applied in the detection field of Ginkgo Damole injection preparation, can solve the problems such as the inability to evaluate the content of active ingredients and the like

Active Publication Date: 2015-04-29
GUIZHOU YIBAI PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method only uses rutin as a control, and it cannot be well calibrated and corrected for flavonoids such as quercetin and terpenoid lactones such as ginkgolide A in Ginkgo biloba extract, and cannot evaluate the content of its active ingredients.

Method used

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  • Method for detecting ginkgo leaf extract and diphyridamole injection preparation
  • Method for detecting ginkgo leaf extract and diphyridamole injection preparation
  • Method for detecting ginkgo leaf extract and diphyridamole injection preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0202] Example 1: Determination of fingerprints in Ginkgo Damo injection based on flavonoids from Ginkgo biloba extract by liquid chromatography:

[0203] (1) Preparation of the test solution: Accurately weigh 10ml of Ginkgo Damo injection to be tested, evaporate to dryness, place 25ml of methanol-25% hydrochloric acid solution (4:1) mixed solution, heat and reflux for 30 minutes, let cool, and filter Transfer to a 25ml measuring bottle, add methanol to the mark, shake well, and use it as the test solution;

[0204] (2) Preparation of the reference solution: Precisely weigh rutin, kaempferol rutinoside, isorhamnetin rutinoside, kaempferol, quercetin, and isorhamnetin, add methanol to dissolve and make each 1ml contain 0.03mg A solution of rutin, 0.02mg kaempferol rutinoside, 0.02mg isorhamnetin rutinoside, 0.03mg kaempferol, 0.03mg quercetin, and 0.02mg isorhamnetin was used as the reference solution;

[0205] (3) Chromatographic conditions: The chromatographic column uses octa...

Embodiment 2

[0212] Example 2: Determination of fingerprints based on the characteristics of terpenoid lactones in Ginkgo biloba extract in Ginkgo Damo injection by liquid chromatography:

[0213] (1) Preparation of test solution: get 20ml of Ginkgo Damo injection to be tested, add 2 drops of 2% hydrochloric acid solution, shake and extract 4 times with ethyl acetate, and the consumption of each ethyl acetate is 15, 10 . 20ml, take the washing liquid separately, wash with 10ml of ethyl acetate, combine the ethyl acetate liquid, recover the ethyl acetate to dryness, add methanol to dissolve the residue and transfer it to a 5ml measuring bottle, add methanol to the mark, shake well, and serve as Test solution;

[0214] (2) Preparation of reference substance solution: Take appropriate amount of ginkgolide A, B, C and bilobalide, weigh them accurately, dissolve them in methanol to make each 1ml contain 1mg ginkgolide A, 1mg ginkgolide B, 1mg Ginkgolide C, the solution of 2mg bilobalide, as r...

Embodiment 3

[0222] Example 3: Determination of fingerprints based on ginkgo leaf extract flavonoids in Ginkgo Damole for Injection by liquid chromatography:

[0223] (1) Preparation of the test solution: Accurately weigh the lyophilized powder of Ginkgo biloba to be tested to contain 4 mg of dipyridamole, directly put 25 ml of methanol-25% hydrochloric acid solution (4:1) mixed solution, and heat to reflux After 30 minutes, let cool, filter and transfer to a 25ml measuring bottle, add methanol to the mark, shake well, and use it as the test solution;

[0224] (2) Preparation of the reference solution: Precisely weigh rutin, kaempferol rutinoside, isorhamnetin rutinoside, kaempferol, quercetin, and isorhamnetin, add methanol to dissolve and make each 1ml contain 0.03mg A solution of rutin, 0.02mg kaempferol rutinoside, 0.02mg isorhamnetin rutinoside, 0.03mg kaempferol, 0.03mg quercetin, and 0.02mg isorhamnetin was used as the reference solution;

[0225] (3) Chromatographic conditions: th...

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Abstract

The invention provides a method for detecting a ginkgo leaf extract and diphyridamole injection preparation. The method comprises the step of fingerprint test and content test of the ginkgo leaf extract and diphyridamole injection preparation, wherein the fingerprint test refers to fingerprint test of flavonoid and terpene lactones in a gingko leaf extract; and the content test refers to test of flavonoid and terpene lactones in the gingko leaf extract. Compared with the prior art, the method can realize more effective product quality control, as well as authentication and content test of effective component monomers, of the ginkgo leaf extract and diphyridamole injection preparation, is very good in precision, stability and repeatability and is another excellent guarantee for the variety curative effect.

Description

Technical field: [0001] The invention relates to a detection method of ginkgo damole injection preparation, which belongs to the technical field of medicines. technical background: [0002] Cardiovascular and cerebrovascular diseases such as coronary heart disease, cerebral thrombosis, hypertension, and cerebral infarction are among the most common and harmful diseases in the world today. One of the reasons; according to reports, the incidence rate has been increasing year by year in recent years, and the number of middle-aged and young patients is increasing. Ginkgo Damole Injection is made from Ginkgo biloba extract and dipyridamole. It is recorded in National Standards for Local Standards of Chemical Drugs (Volume 1), Standard Number: WS-10001-(HD-0087)- 2002. This product can resist platelet aggregation, belongs to coronary artery dilator, and is suitable for the prevention and treatment of coronary heart disease and thromboembolic diseases. To improve myocardial and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 窦啟玲杨青波陈伟陆煜玫刘秋
Owner GUIZHOU YIBAI PHARMA CO LTD
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