Preparation method and product of minimal acid water soluble ginkgo leaf extract

A technology of ginkgo biloba leaves and extract, applied in the field of traditional Chinese medicine extraction, can solve the problems of high ginkgolic acid content, poor water solubility, etc., and achieve the effects of less production input, stable product quality, and strong production operability.

Inactive Publication Date: 2012-02-01
GUILIN HONGHUI PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention aims at the two major problems of high ginkgolic acid content and poor water solubility in ginkgo leaf extracts in the prior art, and uses water as a solvent to extract the active ingredients in ginkgo leaves, and utilizes a method for removing ginkgolic acid from being insoluble in water and refrigerating and precipitating , the content of ginkgolic acid in the obtained ginkgo leaf extract is less than 0.1ppm, the solubility in cold water is greater than 5g / 100ml, and the residue is less than 0.3%

Method used

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  • Preparation method and product of minimal acid water soluble ginkgo leaf extract

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Embodiment 1

[0022] Embodiment 1 of the present invention: get the dry ginkgo leaf 400kg that sells in Pizhou City, Jiangsu Province, drop into 6 tons of extraction ponds, add 4 tons of deionized water and soak for 1 hour, heat and boil to extract for 2 hours, drain the extract; Ginkgo leaf residue Add an equal amount of water to repeat the extraction once, the water extracts are combined and concentrated to 1 ton, put into a 2 ton storage tank and let stand for cooling for 15 hours, take the upper layer solution and add 10% chitin solution to flocculate to form a precipitate, filter with diatomaceous earth to remove the precipitate, The filtered supernatant was put on an AB-8 macroporous resin column, washed with 1.2 tons of deionized water to remove polar impurities, eluted with 2.4 tons of 40%-50% ethanol solvent, concentrated to no ethanol, and refrigerated at low temperature (1°C) for 15 Hours until the feed liquid temperature is 5°C. Take the supernatant and filter it with diatomaceo...

Embodiment 2

[0023] Embodiment 2 of the present invention: get the drying ginkgo leaf 500kg that sells in Pizhou City, Jiangsu Province, drop into 6 tons of extraction ponds, add 5 tons of deionized water and soak for 1 hour, heat and boil and extract for 2 hours, drain the extract; Ginkgo leaf residue Add water and repeat the extraction once, the water extracts are combined and concentrated to 1 / 5 of the original volume, put into a 2-ton storage tank and let stand for cooling for 13 hours, take the upper layer solution and add 10% gelatin solution to flocculate to form a precipitate, filter with diatomaceous earth to remove the precipitate, Filter the clear liquid onto a DM130 macroporous resin column, wash away polar impurities with 1.5 tons of deionized water, elute with 2.5 tons of 40% to 50% ethanol solvent, concentrate the eluent to no ethanol, and refrigerate at low temperature (0°C to 1°C) 13 hours until the feed liquid temperature is 3°C. The supernatant was filtered with diatomac...

Embodiment 3

[0024]Embodiment 3: Get 300kg of dry ginkgo leaves, drop into the extraction pool, add 3 tons of deionized water, heat and boil for extraction for 2 hours, and drain the extract; add water to the ginkgo leaf residue and repeat the extraction once, and the water extracts are combined and concentrated to 1% of the original volume. / 4, put it in the storage tank and let it stand for cooling for 18 hours, take the upper layer solution and add 10% chitosan solution for flocculation and precipitation, filter the precipitation, put the supernatant on the D101 macroporous resin column, and use 2 times the column volume of deionized Wash with water to remove polar impurities, elute with 40% to 50% ethanol solvent of 5 times the column volume, concentrate the eluate to remove ethanol, and refrigerate at low temperature (0°C to 1°C) for 18 hours until the temperature of the feed liquid is 4°C. The supernatant was filtered with diatomaceous earth, the filtrate was concentrated in vacuo to ...

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Abstract

The invention discloses a preparation method and product of minimal acid water soluble ginkgo leaf extract. According to the method, the ginkgo leaf is extracted by using water as a solvent, the content of ginkgolic acid is reduced by flocculating settling combined with refrigeration, and gingko flavone and terpene lactone are purified and concentrated by resin column chromatography. The content of ginkgolic acid of the product is less than 0.1ppm, the solubility of the product in cold water is 5g/100ml, and two main problems of high content of ginkgolic acid and poor water solubility of ginkgo leaf extract are solved simultaneously. Compared with the prior art, the extraction and preparation process disclosed by the invention has the advantages of little production investment, simplicityof equipment, flow and operation, low production cost, safety of used reagents, no intervention of toxic organic solvent, stable product quality, strong operability in industrialized production and the like.

Description

technical field [0001] The present invention relates to a preparation method and product of ultra-low-acid water-soluble ginkgo leaf extract, especially a method for extracting and preparing ginkgo leaf extract with ginkgolic acid less than 0.1ppm and cold water solubility greater than 5g / 100ml from ginkgo leaves, which belongs to traditional Chinese medicine Extract technology fields. Background technique [0002] Ginkgo biloba L. is a gymnosperm of the genus Ginkgo. Our country is extremely rich in Ginkgo biloba resources, accounting for about 70% of the world's Ginkgo biloba resources. Ginkgo biloba extract is a strong free radical scavenger, which can improve senile dementia, aging, and neuropathy. Ginkgo biloba preparations are currently mainly used clinically for middle-aged and elderly brain insufficiency and senile dementia caused by coronary heart disease, angina pectoris, myocardial infarction, stroke, cerebral circulation disorder and brain function degeneration....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/16A61K127/00
Inventor 黄五久
Owner GUILIN HONGHUI PHARMA CO LTD
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