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Preparation method of low-acid and high-quality ginkgo leaf extractive

A technique for ginkgo biloba leaves and extracts, which is applied in the field of preparation of low-acid and high-quality ginkgo biloba extracts, can solve problems such as adverse reactions, cumbersome extraction process, uncontrolled extraction, etc., and achieves fewer extraction process steps, high enrichment extraction rate, Reasonable effect of the process route

Active Publication Date: 2011-07-27
JIANGSU SHENLONG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the existing technologies only focus on the purification of the extract, pursue the high content of active ingredients, and ignore the content control of the harmful component ginkgolic acid, which is likely to cause adverse reactions in clinical use
In order to solve this problem, there are a few reports that increase the content of active ingredients and reduce the content of harmful ingredients. Ginkgo biloba extract, but the extraction process is cumbersome, and some need to use noble fillers such as dextran gel, which cannot achieve industrialized mass production.
Secondly, the current active ingredients only focus on ginkgo flavonol glycosides and terpene lactones, while the literature reports that stilbenes have anti-oxidation, blood lipid lowering and other effects, and have a good protective effect on the cardiovascular system. It may be Ginkgo extract Another large class of active ingredients for the treatment of cardiovascular and cerebrovascular diseases
However, all reported literature patents do not control the content of 3,3'-dimethoxy-4,4'-dihydroxystilbene

Method used

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  • Preparation method of low-acid and high-quality ginkgo leaf extractive
  • Preparation method of low-acid and high-quality ginkgo leaf extractive
  • Preparation method of low-acid and high-quality ginkgo leaf extractive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032]1 kg of dried ginkgo leaves, crushed to pass through a 20-mesh sieve, reflux extracted three times with 70% ethanol by mass percentage, added alcohol 14 times the weight of dried ginkgo leaves for the first time, extracted for 1 hour, added the weight of dried ginkgo leaves for the second and third times respectively 12. Extract 8 times the amount of alcohol for 1.5 hours, combine the extracts, filter, recover ethanol under reduced pressure, and concentrate to a thick paste (relative density 1.15 at 60°C). Add water in an amount 3 times the weight of the dried ginkgo leaves, stir well, put it in a cold storage at 4°C for 48 hours, and centrifuge to obtain a supernatant. With the ratio of adding 2ml supernatant per gram of resin, pass through the ion exchange column composed of mixed resin (wherein the column packing ratio of macroporous adsorption resin DM130 and polyamide resin is 9:1), and the column diameter-height ratio is 1: 6. First wash with 4 times the column vo...

Embodiment 2

[0034] Dried ginkgo leaves 5kg, crushed to pass through a 20 mesh sieve, reflux extracted three times with 80% ethanol by mass percentage, added 12 times the weight of dry ginkgo leaves for the first time, extracted for 2 hours, and 10 and 6 times the amount for the second and third times respectively , were extracted for 1.5 hours, the combined extracts were filtered, ethanol was recovered under reduced pressure, and concentrated to a thick paste (relative density at 60°C was 1.17). Add water 5 times the weight of the leaf, stir well, put it in the cold storage for 24 hours, centrifuge to obtain the supernatant. Add the ratio of 2.5ml supernatant to every gram of resin, pass through the ion exchange column (wherein the packing ratio of macroporous adsorption resin AB-8 and polyamide resin is 8: 1) that has been made up of mixed resin, pack The ratio of column diameter to height is 1:7. First wash with 4 times the column volume of distilled water, then use 6 times the column ...

Embodiment 3

[0036] 50 kg of dried ginkgo leaves were crushed through a 20-mesh sieve, and 85% ethanol was used for reflux extraction three times, the first time adding 13 times the weight of dry ginkgo leaves, extracting for 1.5 hours, the second and third times were respectively 11 and 7 times the amount, All extracts were extracted for 1 hour, the extracts were combined, filtered, ethanol was recovered under reduced pressure, and concentrated to a thick paste (relative density at 60°C was 1.20). Add water 5 times the weight of the leaves, stir well, put it in the cold storage for 48 hours, centrifuge to obtain the supernatant. Add 2ml of supernatant per gram of resin, and pass through the ion exchange column composed of mixed resin that has been treated (the column packing ratio of macroporous adsorption resin D101 and polyamide resin is 9:1), and the column diameter is high. The ratio is 1:5. Wash with 3 times the column volume of distilled water, then use 6 times the column volume of...

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Abstract

The invention discloses a preparation method of a low-acid and high-quality ginkgo leaf extractive, which comprises the steps of extraction, fine purification and separation and concentrated drying. In the ginkgo leaf extractive obtained in the method, the total content of flavones is up to over 65% (wherein a flavonoide content is up to over 35%), the total content of terpene lactone is up to over 10%, the content of 3,3'-dimethoxy-4,4'-dyhydroxy diphenylethene is up to over 0.05%, and the content of ginkgoic acid can be less than 2ppm. Compared with the prior art, in the invention, the content of the 3,3'-dimethoxy-4,4'-dyhydroxy diphenylethene is quantitatively controlled for the first time, the low-acid and high-quality ginkgo leaf extractive has high enrichment and extraction rate ofactive constituents, low content of the ginkgoic acid as a harmful constituent and has the characteristics that a production process meets the requirement of industrial large-scale production, and a process route is more reasonable.

Description

technical field [0001] The invention relates to a preparation method of a low-acid high-quality ginkgo leaf extract, which can be used as a treatment for dizziness caused by blood stasis type thoracic obstruction and blood stasis type mild cerebral arteriosclerosis, coronary heart disease, angina pectoris, myocardial infarction and other diseases, belonging to the technical field of traditional Chinese medicine preparation. Background technique [0002] Cardiovascular and cerebrovascular diseases rank first among various causes of death, and have become the number one killer with the highest cause of death for human beings. According to statistics, the population over the age of 60 in China has exceeded 300 million, and the advent of population aging has led to a corresponding increase in the number of patients with cardiovascular and cerebrovascular diseases. At the same time, pressure from work, society, and life in modern society has led to an increasing incidence of car...

Claims

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Application Information

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IPC IPC(8): A61K36/16A61P9/10
Inventor 胡传良秦勇段绪红金春徐成
Owner JIANGSU SHENLONG PHARMA
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