Preparation method of ginkgo leaf biological feed additive

A biological feed, ginkgo biloba technology, applied in animal feed, animal feed, application and other directions, can solve the problem of less research on ginkgo biloba, improve immunity and antioxidant capacity, improve intestinal micro-ecological environment, and improve anti-oxidation. The effect of sickness

Inactive Publication Date: 2013-04-03
NANJING FORESTRY UNIV
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Problems solved by technology

Ginkgo biloba and its extracts have been widely used in the fields of medical treatment, food preservativ

Method used

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  • Preparation method of ginkgo leaf biological feed additive
  • Preparation method of ginkgo leaf biological feed additive
  • Preparation method of ginkgo leaf biological feed additive

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[0026] The preparation method comprises the following steps:

[0027] Step S101, mixing the solid fermentation raw material and the nutrient salt solution of the microorganism evenly, and obtaining the solid fermentation base material after sterilization;

[0028] Step S102, using the probiotic Bacillus as the strain, expanding the strain and inoculating it into the solid fermentation base material prepared in step S101 for fermentation,

[0029] Step S103, drying and pulverizing the fermented material to obtain the product.

[0030] In the embodiment of the present invention, the parts by weight of the components of the solid fermentation raw material in step S101 are: 5-8 parts of crushed ginkgo leaves, 1-3 parts of soybean meal and 1-2 parts of bran, among which 7 parts of ginkgo leaves are preferred. 1 part, 2 parts of soybean meal and 1 part of bran.

[0031] In the embodiment of the present invention, the probiotics used in step S102 are spore probiotics.

[0032] In ...

Embodiment 1

[0054] Embodiment 1: the ginkgo leaf solid-state fermentation of bacillus licheniformis

[0055] 1, the preparation of nutrient salt solution: according to glucose 1.5% (that is, every 100mL nutrient salt solution contains 1.5g of glucose, the same below), K 2 HPO 4 0.3%, MnSO 4 0.02%, MgSO 4 ·7H 2 O 0.05%, FeCl 3 The amount of 0.01% is weighed in turn and dissolved in water to make a nutrient salt solution;

[0056] 2, the preparation of solid medium: take by weighing three by the ratio of Ginkgo biloba powder: soybean meal: bran=7: 2: 1, mix;

[0057] 3. Mix the above two according to the ratio of 10g of solid medium plus 12mL of nutrient salt solution, stir evenly, and prepare a fermentation medium; sterilize the prepared fermentation medium at 121°C for 1 hour, take it out, and cool it down;

[0058] 4. Carry out routine aseptic operation in the inoculation room, inoculate 2 mL of Bacillus licheniformis per 10 g of fermentation medium (the culture of Bacillus licheni...

Embodiment 2

[0060] Embodiment 2: Ginkgo leaf solid-state fermentation of bacillus natto

[0061] 1, the preparation of nutrient salt solution: according to glucose 1.5% (that is, every 100mL nutrient salt solution contains 1.5g of glucose, the same below), K 2 HPO 4 0.3%, MnSO 4 0.02%, MgSO 4 ·7H 2 O 0.05%, FeCl 3 The amount of 0.01% is weighed in turn and dissolved in water to make a nutrient salt solution;

[0062] 2, the preparation of solid medium: take by weighing three by the ratio of Ginkgo biloba powder: soybean meal: bran=7: 2: 1, mix;

[0063] 3. Mix the above two according to the ratio of 10g of solid medium plus 12mL of nutrient salt solution, stir evenly, and prepare a fermentation medium; sterilize the prepared fermentation medium at 121°C for 1 hour, take it out, and cool it down;

[0064] 4. Carry out routine aseptic operation in the inoculation room, inoculate 2 mL of Bacillus natto per 10 g of fermentation medium (the culture of Bacillus licheniformis adopts liquid...

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Abstract

The invention discloses a preparation method of a ginkgo leaf biological feed additive. The preparation method comprises the following steps: with the ginkgo leaf as a main solid state fermentation substrate and probiotic bacillus as a strain, performing solid state fermentation under suitable nutrient conditions and culture conditions; and drying and crushing to obtain the biological feed additive. The biological feed additive contains a variety of bioactive substances such as flavonoid compounds and terpene lactone and can improve the immunity and the antioxidant capacity of an animal body; through biological fermentation, the probiotic bacilli are rich in the biological feed additive, so that the biological feed additive can adjust the intestinal flora balance of the animal, improve the intestinal microecological environment, promote the animal growth, reduce the intestinal diseases of the animal, and improve the disease resistance of the animal body; and the biological feed additive can significantly improve the productivity of livestock and poultry, and can substitute or partially substitute antibiotics, thus providing a health guarantee for consumption and use of livestock and poultry food.

Description

technical field [0001] The invention belongs to the technical field of biological feed development, in particular to a preparation method of ginkgo biloba biological feed additive. Background technique [0002] Traditional antibiotics can significantly improve the production performance of livestock and poultry, but the residues of these antibiotics in livestock and poultry are likely to cause food safety problems, and natural plant feed additives are green, safe, and have no or low toxic and side effects. Therefore, the development of natural As a substitute for antibacterial drugs, plant resources have become one of the research hotspots in the field of feed. [0003] Ginkgo biloba, also known as ginkgo, is a monotypic species of the gymnosperm Ginkgo family. Ginkgo leaves contain flavonoids, terpenoids, phenols and other active ingredients, which have antibacterial, anti-inflammatory, antiviral, and antioxidant properties. Ginkgo biloba and its extracts have been widely ...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/14
Inventor 赵林果曹福亮高树峰张旭晖汪贵斌
Owner NANJING FORESTRY UNIV
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