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Cyp7lav1 gene promoter

A promoter and gene technology, applied in the direction of DNA/RNA fragments, the use of vectors to introduce foreign genetic material, recombinant DNA technology, etc., can solve the problems such as the undiscovered cyp71av1 gene promoter sequence report

Inactive Publication Date: 2009-07-08
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] After searching the prior art documents, it is found that there is no report related to the cyp71av1 gene promoter sequence of the present invention

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0034] Step 1, Cultivate Artemisia annua sterile vaccine

[0035] Artemisia annua seeds were soaked in 75% ethanol for 1 min, then soaked in 20% (w / v) NaClO for 20 min, rinsed with sterile water for 3-4 times, blotted the surface moisture with sterile absorbent paper, and inoculated Cultivate on hormone-free MS solid medium at 25°C under 16h / 8h (light / dark) light, and 14 days later, you can obtain the sterile vaccine of Artemisia annua.

[0036] Step 2: small amount of extraction of total DNA from Artemisia annua leaves by CTAB method

[0037] Take about 100mg of leaves into a 1.5mL EP tube, add 600 μL of preheated CTAB buffer, the buffer contains 2% β-mercaptoethanol, grind the leaves with a grinding rod until there are no obvious particles; place the EP tube at 65 ℃ water bath for 45 minutes to fully lyse the cells. After the solution is cooled, add 600 μL of chloroform-isoamyl alcohol mixed solution, the volume ratio of chloroform to isoamyl alcohol in the mixed solution ...

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PUM

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Abstract

A cyp71av1 gene promoter relates to the field of genetic engineering technique, the promoter sequence has a nucleotide sequence in the 2100bp of SEQ ID NO.1 in the sequence table; the promoter can lead the specific expression of the gene in the plant tentacle, and can be applied in the genetic engineering breeding using the plant tentacle tissue expression and producing metabolites. The cyp71av1 gene promoter obtained by the invention can lead the specific expression of the gene in the plant tentacle, and has great significance to the genetic engineering breeding using the plant tentacle tissue expression and producing metabolites.

Description

technical field [0001] The present invention relates to a gene promoter in the technical field of genetic engineering, in particular to a cyp71av1 gene promoter. Background technique [0002] Artemisia annua L. is an annual herb belonging to the family Asteraceae. Artemisinin is a sesquiterpene lactone compound containing a peroxide bridge structure isolated from its aerial part. It is currently recognized as the most effective drug for treating malaria in the world, especially for cerebral malaria and chloroquine-resistant malaria. efficient. Currently, the most effective treatment for malaria recommended by the World Health Organization is artemisinin combination therapy (ACTs). In addition, with the gradual deepening of pharmacological research on artemisinin, scientists have found that artemisinin and its derivatives also have anti-inflammatory, anti-schistosomiasis, anti-tumor and immune regulation functions. It can be seen that artemisinin is a very potential natura...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12N15/82C12N15/113
Inventor 唐克轩王鵫月唐岳立
Owner SHANGHAI JIAO TONG UNIV
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