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AaADH1 gene promoter and application and preparation method thereof

A promoter and gene technology, applied in the field of genetic engineering, can solve problems such as the undiscovered AaADH1 gene promoter sequence

Active Publication Date: 2017-01-04
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After searching the prior art literature, it is found that there is no report related to the AaADH1 gene promoter sequence of the present invention

Method used

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  • AaADH1 gene promoter and application and preparation method thereof
  • AaADH1 gene promoter and application and preparation method thereof
  • AaADH1 gene promoter and application and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0042] This embodiment relates to the acquisition of the AaADH1 gene promoter, which specifically includes the following steps:

[0043] Step 1, Cultivate Artemisia annua sterile vaccine

[0044] Artemisia annua seeds were soaked in ethanol with a volume fraction of 75% for 1 min, then soaked in 20% (w / v) NaClO for 20 min, rinsed with sterile water for 3 to 4 times, blotted the surface moisture with sterile absorbent paper, and inoculated Cultivate on a hormone-free MS solid medium at 25°C under 16h / 8h (light / dark) light, and after 14 days, you can obtain an Artemisia annua sterile vaccine;

[0045] Step 2, according to the AaADH1 gene promoter sequence in the whole genome of Artemisia annua, the promoter sequence was cloned by nested PCR;

[0046] In order to improve the specificity of the product, two rounds of nested PCR amplification were used, and the primers used were shown in Table 2. AaproADH1-FP1 (SEQ ID NO: 2) and AaproADH1-RP1 (SEQ ID NO: 3) were derived from Artem...

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Abstract

The invention discloses an AaADH1 gene promoter and a preparation method. The nucleotide sequence of the promoter is shown in SEQ iD NO:1. The invention further relates to application of the AaADH1 gene promoter in genetic engineering breeding utilizing plant glandular hair tissue expression and metabolite production. The promoter can guide genes to be specifically expressed in plant glandular hair, due to the fact that the plant glandular hair specifically-expressed promoter carries out genetic manipulation on a plant glandular hair system, no harm is caused to growth and development of plants, and the AaADH1 gene promoter has great significance to genetic engineering breeding utilizing plant glandular hair tissue expression and metabolite production.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a gene promoter and its application and preparation method. Background technique [0002] Artemisia annua L. is an annual herb belonging to the family Asteraceae. Artemisinin, a sesquiterpene lactone oxide containing a peroxy bridge extracted from its aerial part, is currently the most widely used and effective antimalarial drug, especially for cerebral malaria and anti-chloroquine malaria. . Currently, artemisinin combination therapies (ACTs) are the most effective treatment for malaria recommended by the World Health Organization. However, the content of artemisinin in the plant Artemisia annua is low, which cannot fully meet the global market demand. Artemisia annua has glandular trichomes and nonglandular trichomes. The two kinds of glandular trichomes play a vital role in the growth, development, defense, and pollen dispersal of plants, and are closely relate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N1/21C12N15/82C12N15/66A01H5/00C12R1/01
CPCC12N9/0006C12N15/8205C12N15/8243
Inventor 唐克轩何倩付雪晴石璞沈乾黎凌孙小芬
Owner SHANGHAI JIAO TONG UNIV
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