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AaDBR2 gene promoter DBR2pro1 and applications thereof

A technology of promoters and genes, applied in the field of promoters, can solve problems such as no reports of promoters

Inactive Publication Date: 2017-02-15
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the promoters of AaADS and AaCYP have been cloned, but the promoter of AaDBR2 has not been reported

Method used

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  • AaDBR2 gene promoter DBR2pro1 and applications thereof
  • AaDBR2 gene promoter DBR2pro1 and applications thereof
  • AaDBR2 gene promoter DBR2pro1 and applications thereof

Examples

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Embodiment Construction

[0029] The present invention will be described in detail below in conjunction with specific embodiments. The following examples will help those skilled in the art to further understand the present invention, but do not limit the present invention in any form. It should be noted that those skilled in the art can make several modifications and improvements without departing from the concept of the present invention. These all belong to the protection scope of the present invention.

[0030] The experimental method that does not indicate specific condition in the following examples, usually according to conventional conditions, such as molecular cloning such as Sambrook: laboratory manual sees the condition described in the 1989 edition of New York:Cold Spring Harbor Laboratory Press, or according to manufacturer's suggested conditions. The Agrobacterium tumefaciens EHA105 involved in this embodiment has been described in "Huang Yali, Jiang Xiliang, Tian Yunlong, Guo Ping, Zhu ...

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Abstract

The invention provides an AaDBR2 gene promoter DBR2pro1 and applications of the AaDBR2 gene promoter DBR2pro1, belonging to the technical field of genetic engineering. The nucleotide sequence of the promoter is shown as SEQ ID NO.1. The invention further relates to the applications of the AaDBR2 gene promoter DBR2pro1 in genetic engineering breeding utilizing plant secretion glandular hair tissue expression and production metabolites. The AaDBR2 gene promoter DBR2pro1 provided by the invention can guide the exogenous gene to be specifically expressed in the plant secretion glandular hair, thus having great significance in the genetic engineering breeding utilizing plant secretion glandular hair tissue expression and production metabolites.

Description

technical field [0001] The invention relates to a promoter specifically expressed in plant secretory trichomes and its use, in particular to an AaDBR2 gene promoter DBR2pro1 and its use. Background technique [0002] Artemisia annua L. is an annual herb belonging to the family Asteraceae. Artemisinin is a sesquiterpene lactone compound containing a peroxide bridge structure isolated from its aerial part. It is currently recognized as the most effective drug for treating malaria in the world, especially for cerebral malaria and chloroquine-resistant malaria. efficient. Currently, the most effective treatment for malaria recommended by the World Health Organization is artemisinin combination therapy (ACTs). At present, the main source of artemisinin is extracted from the aerial parts of Artemisia annua plants. However, the content of artemisinin in Artemisia annua is very low (0.01%-1%), which limits the large-scale commercial production of this drug. As the biosynthetic pa...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/82A01H5/00
CPCC12N9/0008C12N15/8223C12N15/8243
Inventor 唐克轩江伟民陆续石璞郝小龙王国丰
Owner SHANGHAI JIAO TONG UNIV
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