Efficient germination method of leymus chinensis seeds

A seed and Leymus chinensis technology, applied in the field of seed biology, can solve the problems of poor comparability between data, non-reproducibility, time-consuming and labor-intensive, etc. Effect

Pending Publication Date: 2017-05-31
PRATACULTURE INST HEILONGJIANG ACAD OF AGRI SCI
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the technical problems that current experimental varieties for germination of Leymus chinensis seeds are limited to a single selected variety, poor comparability between data, time-consuming and labor-intensive and non-repeatable, and provide a method for efficient germination of Leymus chinensis seeds

Method used

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specific Embodiment approach 1

[0021] Specific embodiment one: present embodiment is a kind of method for efficient germination of Leymus chinensis seeds, specifically carried out according to the following steps:

[0022] 1. Select seeds: collect Leymus chinensis seeds at the stage of milk maturity, then dry them in the sun, and select Leymus chinensis seeds with a purity of more than 95% for later use; said Leymus chinensis seeds have not undergone debran treatment;

[0023] 2. Wall breaking and ultrasonication: put Leymus chinensis seeds with a seed purity of more than 95% in step 1 into an aqueous sodium hydroxide solution with a mass fraction of 30% to 35% and soak for 40min to 45min, then wash with sterile water for 3 ~4 times, dry at room temperature for 24h~30h, ultrasonic treatment for 20min~25min;

[0024] 3. Prepare medium: add sucrose, gibberellin and agar to 1 / 2MS medium, sterilize at 121°C for 15 minutes, adjust pH to 5.8-6.0 with sodium hydroxide and hydrochloric acid, and obtain SMG medium; ...

specific Embodiment approach 2

[0027] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the Leymus chinensis seeds described in step 1 are Nongjing No. 4, Nongjing No. 11, Jisheng No. 1, Northeast Leymus chinensis or Hailaer Wild Leymus chinensis . Others are the same as the first embodiment.

specific Embodiment approach 3

[0028] Specific embodiment three: the difference between this embodiment and specific embodiment one is: in step two, the Leymus chinensis seeds whose seed clarity in step one is more than 95% are put into the sodium hydroxide aqueous solution whose mass fraction is 30%. 40min. Others are the same as the first embodiment.

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Abstract

The invention relates to an efficient germination method of leymus chinensis seeds and aims to solve the technical problems that experiment varieties for germination of leymus chinensis seeds are limited to bred single varieties, have poorer data comparability, are time-consuming and effort-consuming and have no repeatability at present. The method comprises the steps as follows: 1, seed selection; 2, wall breakage and ultrasonic treatment; 3, preparation of a culture medium; 4, soaking with an ethanol aqueous solution and sterilization with sodium hypochlorite; 5, culture in the culture medium. The method has the advantages as follows: 1) germination of leymus chinensis can be not limited by season and environment and has the advantages of being simple, convenient, quick, efficient, seldom restricted by varieties and the like; 2) dormancy of the leymus chinensis seeds is broken through effective combination of physical, chemical and hormone for the first time, and the germination rate of the leymus chinensis is significantly increased, so that sufficient seedlings can be obtained to be applied to establishment of a leymus chinensis regeneration system and a genetic transformation system and commercial production of pasture.

Description

technical field [0001] The invention relates to a method for germination of grass seeds, in particular to a method for efficient germination of Leymus chinensis seeds, and belongs to the technical field of seed biology. Background technique [0002] Leymus chinensis, also known as Leymus chinensis, belongs to the rhizome-type perennial gramineous herbage of Lymus genus in Poaceae, and is one of the main constructive species and dominant species in grasslands in Northeast my country and Inner Mongolia. The grassland area of ​​Leymus chinensis in my country is about 210,000 square kilometers, accounting for 50% of the total area of ​​the world. Leymus chinensis has strong adaptability, has excellent characteristics such as cold resistance, drought and salinity, and has high nutritional value, good palatability, high feeding value and economic value. Commonly used species for land degradation, restoration and reconstruction of grassland ecosystems. [0003] At present, the ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00
CPCA01C1/00
Inventor 王建丽付春祥申忠宝曹英萍钟鹏潘多锋张瑞博李道明张海玲
Owner PRATACULTURE INST HEILONGJIANG ACAD OF AGRI SCI
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