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Genetic engineering application of nitrate transport protein genom OsNRT2.3 in Rice

A genetic engineering and nitrate technology, which is applied to the application of genes encoding nitrate absorption and its regulation in rice. In the field of genetic engineering applications, it can solve the problems of nitrogen loss and low nitrogen fertilizer utilization rate, and achieve the effect of improving adaptability.

Active Publication Date: 2009-03-25
NANJING AGRICULTURAL UNIVERSITY
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  • Application Information

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Problems solved by technology

There are a series of environmental problems caused by low nitrogen use efficiency and a large amount of nitrogen loss in China.

Method used

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  • Genetic engineering application of nitrate transport protein genom OsNRT2.3 in Rice
  • Genetic engineering application of nitrate transport protein genom OsNRT2.3 in Rice
  • Genetic engineering application of nitrate transport protein genom OsNRT2.3 in Rice

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Embodiment Construction

[0029] 1. Obtaining the gene sequence

[0030] The amino acid sequence of the Arabidopsis high-affinity nitrate transport protein gene AtNRT2.1 was obtained by searching on the NCBI website (http: / / www.ncbi.nlm.nih.gov / ), and the amino acid sequence of AtNRT2.1 was used as the comparison The correct template is on the KOME website ( http: / / cdna01.dna.affrc.go.ip / cDNA / ) and obtained 4 highly homologous amino acid sequences in rice. Through the homology analysis of the full-length genome sequence of the above four amino acid sequences, it was found that two of the amino acid sequences were derived from a genome sequence, and we defined this genome sequence as OsNRT2.3. The two amino acid sequences encoded by it are OsNRT2.3a and OsNRT2.3b respectively. And obtained these two full-length cDNA clones from the KOME website (http: / / cdna01.dna.affrc.go.jp / cDNA / ), the accession number of OsNRT2.3a gene is AK109776; the accession number of OsNRT2.3b gene is AK072215 , located on t...

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Abstract

The invention discloses a genetic engineering application of nitrate transport protein genome OsNRT2.3 of paddy rice, pertaining to the genetic engineering field. The nucleotide sequence of the nitrate transport protein genome OsNRT2.3 of paddy rice is SEQ ID NO.1, and OsNRT2.3a protein amino acid sequence expressed thereby is SEQ ID NO.2 and OsNRT2.3b protein amino acid sequence expressed thereby is SEQ ID NO.3. The gene of the invention is a first report on paddy rice. By participation in the transport of nitrate of paddy rice and especially under the condition short of nitrogen and poor pHenvironment, the analysis of mRNA expression shows that OsNRT2.3a is induced by low nitrate nitrogen so that the expression only occurs in lateral roots; and OsNRT2.3b has expressions in root system and parts over the ground and is responsible for the transport of nitrate. The OsNRT2.3a and OsNRT2.3b proteins are expressed in a frogspawn heterogenous system and determined as high compatible nitrate transport protein. A pH regulation and control site is found to exist in both proteins which are regulated and controlled by pH. Under low nitrogen condition, OsNRT2.3 transgenic plant shows higherefficiency in transport of nitrate, thus improving the utilization efficiency of nitrogen and the ultimate output.

Description

technical field [0001] The invention discloses the genetic engineering application of rice nitrate transport protein genome OsNRT2.3, belongs to the technical field of genetic engineering, and specifically relates to the application of genes encoding nitrate absorption and its regulation in rice. Background technique [0002] Nitrogen is one of the important macronutrients for crops and participates in various metabolic processes of organisms. It is a component of many living substances in plants, such as: amino acids, proteins, nucleic acids, enzymes, chlorophyll, etc. Nitrogen accounts for 1.5-2% of plant dry weight and 16% of total plant protein (Frink CR., Waggoner PE. and Ausubel JH. Nitrogen fertilizer: retrospect and prospect. Proc. Natl Acad. Sci. USA. 1999.96: 1175 -1180.). At present, China's nitrogen fertilizer use accounts for 30% of the global nitrogen fertilizer use (Peng Shaobing, Huang Jianliang, Zhong Xuhua, Yang Jianchang, Wang Guanghuo, Zou Yingbin, Zhan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/11C07K14/415A01H1/00C12N15/84
Inventor 范晓荣严明徐国华沈其荣
Owner NANJING AGRICULTURAL UNIVERSITY
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