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A transcription factor PwNAC2 related to plant stress tolerance, a coding gene thereof and applications of the transcription factor

A stress tolerance, plant technology, applied in the direction of plant gene improvement, application, plant peptides, etc., can solve the problem of few reports on NAC gene function.

Active Publication Date: 2018-03-23
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, important progress has been made in the research on the relationship between NAC gene and plant stress resistance in model plants such as Arabidopsis and rice, but there are few reports on the function of NAC gene in woody plants

Method used

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  • A transcription factor PwNAC2 related to plant stress tolerance, a coding gene thereof and applications of the transcription factor
  • A transcription factor PwNAC2 related to plant stress tolerance, a coding gene thereof and applications of the transcription factor
  • A transcription factor PwNAC2 related to plant stress tolerance, a coding gene thereof and applications of the transcription factor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Example 1. Acquisition of transcription factor PwNAC2 and its coding gene

[0081] 1. Acquisition of the transcription factor PwNAC2 and its coding gene

[0082] The Gateway method was used to construct the Cinnamon cDNA library, which was completed by Yingwei Jieji (Shanghai) Company. Using the EST sequence of PwNAC2 of P. japonicus as template, 5'-CCCAATCATCCAACCTTAGGCT-3' and 5'-CAACTGGGCCTCTGCATTC-3' primers were used for PCR amplification to obtain 5'RACE-PCR; -CTGCCAGGAAACAGCTATGAC-3'primers were used for PCR amplification to obtain 3'RACE-PCR, and the RACE-PCR product was spliced ​​with EST sequence to obtain the full-length cDNA sequence, which was then sequenced.

[0083] Sequencing results show that: the cDNA nucleotide sequence is sequence 1 in the sequence table, consisting of 1164 nucleotides, compared with the sequence in Genbank, it is determined that it belongs to the NAC transcription factor of C. PwNAC2, the protein encoded by PwNAC2 is named PwNAC2,...

Embodiment 2

[0087] Example 2, Application of PwNAC2 Gene in Improving Plant Stress Tolerance

[0088] 1. Construction of transgenic Arabidopsis thaliana

[0089] 1. Acquisition of PwNAC2 gene

[0090] Prepare the following primer pairs:

[0091] Primer 1: 5'-CAACATGGGGAGACCGAATGC-3';

[0092] Primer 2: 5'-TCAATCCCTCTAATAAGAA-3'.

[0093] Using the cDNA of Pseudomonas japonica as a template, PCR amplification was carried out with the above primers 1 and 2 to obtain a PCR product with a size of 1164bp, which was then sequenced.

[0094] Sequencing results show that the nucleotide sequence of the PCR product is sequence 1 in the sequence listing, which can encode the protein PwNAC2 shown in sequence 2 in the sequence listing.

[0095] 2. Acquisition of recombinant expression vector

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Abstract

A transcription factor PwNAC2 related to plant stress tolerance, a coding gene thereof and applications of the transcription factor are disclosed. Protein provided by the invention is a), b), c) or d), wherein the a) is protein the amino acid sequence of which is shown as a sequence 2; the b) is fusion protein obtained by connecting a label to the N end and / or C end of the protein shown as the sequence 2; the c) is protein having same functions and obtained by substitution and / or deletion and / or addition of one or a plurality of amino acid residues to the amino acid sequence shown as the sequence 2; and the d) is protein which has functions same to functions of the amino acid sequence shown as the sequence 2, and which has 75% or 75% or above of homology with the amino acid sequence shownas the sequence 2. The new gene PwNAC2 is found and is introduced into arabidopsis thaliana to obtain a PwNAC2-transgenicarabidopsis thaliana plant. Experiments prove that drought tolerance and salt tolerance of the PwNAC2-transgenicarabidopsis thaliana plant are significantly improved, thus proving that the PwNAC2 or the protein coded by the PwNAC2 has stress tolerance.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a transcription factor PwNAC2 related to plant stress tolerance, its coding gene and its application. Background technique [0002] Abiotic stresses such as drought and salinity seriously affect the normal growth and development of plants, and plants will develop a series of effective mechanisms during the long-term evolution process, including regulating the expression of stress-related genes to cope with external adverse environments. Stress-induced expression genes can be roughly divided into two categories: functional protein-coding genes and transcriptional regulatory protein-coding genes involved in stress signal transduction. [0003] NAC transcription factor is a plant-specific transcriptional regulator with multiple biological functions, and plays an important role in plant growth and development, morphogenesis, hormone regulation and stress response. At present,...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/82C12N15/29A01H5/00A01H6/20A01H6/00
CPCC07K14/415C12N15/8267C12N15/8273
Inventor 张凌云张鹤华
Owner BEIJING FORESTRY UNIVERSITY
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