Mink growth hormone releasing hormone cDNA and application thereof

A growth hormone and hormone-releasing technology, which is applied in the field of mink growth hormone-releasing hormone cDNA, can solve problems affecting cell activity, etc., and achieve obvious weight gain, accelerated growth, and growth-promoting effects

Inactive Publication Date: 2012-04-04
CHINA AGRI UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

The increase of cAMP level will promote the conformational change and activation of the regulatory subunit of cAMP-d

Method used

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  • Mink growth hormone releasing hormone cDNA and application thereof
  • Mink growth hormone releasing hormone cDNA and application thereof
  • Mink growth hormone releasing hormone cDNA and application thereof

Examples

Experimental program
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Embodiment

[0028] Taking the nucleotide sequence of mink GHRH mature peptide as the target gene and pVAX1 as the eukaryotic expression vector, the target gene is inserted into the vector to construct a eukaryotic expression vector, and injected intramuscularly into the leg muscles of the mink to obtain the growth rate of the mink Accelerated, noticeable results in weight gain.

[0029] The vector construction process is as follows: the signal peptide gene sequence of human growth hormone and the mature peptide gene sequence of mink growth hormone releasing hormone were connected in series by PCR method, connected to the T vector (pMD19-GHRHS), and then double-linked with BamH I and EcoR I respectively. Cut the target gene on the pMD19-GHRHS vector and pVAX1 empty vector. The target gene was cloned into the pVAX1 vector, identified by PCR and sequencing (see figure 1 ), and then the pMD19-GHRHS and pVAX1-GHRHS vectors were digested and identified (see figure 2 and 3 ). It was confirm...

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Abstract

The invention discloses a full-length nucleotide sequence and a mature peptide nucleotide sequence of a mink growth hormone releasing hormone cDNA gene, and encoded protein amino acid sequences thereof. The mink growth hormone releasing hormone cDNA sequence is a complete open reading frame with 321 nucleotide sequences, and 106 amino acids are encoded. The mature peptide is encoded by 132 nucleotide sequences, and 44 amino acid residues are encoded. The mink growth hormone releasing hormone cDNA can be used for promoting the growths of economic animals such as minks.

Description

technical field [0001] The invention relates to mink growth hormone releasing hormone cDNA obtained by cloning and application thereof. Background technique [0002] Mink is a fur animal with high economic value. Its growth is mainly controlled by the growth hormone produced and released by the pituitary gland. After reaching body maturity, the production and release of growth hormone decreases. Growth hormone is under the dual control of growth hormone releasing hormone and growth hormone releasing inhibitory hormone produced by the hypothalamus of the animal itself. Growth hormone-releasing hormone stimulates the production and release of growth hormone by the pituitary gland, while growth hormone-releasing inhibitory hormone inhibits the production of growth hormone. The two work together to maintain the growth rate of mink. Growth hormone-releasing hormone is produced in the arcuate nucleus and ventromedial nucleus of the hypothalamus. After synthesis, it is transporte...

Claims

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Application Information

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IPC IPC(8): C12N15/63C07K14/60C12N1/19C12N1/21C12N5/10A61K38/25C12N15/16C12N1/15A61P43/00A61K48/00
Inventor 王吉贵刘维全刘芃芃齐顺章
Owner CHINA AGRI UNIV
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