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Iron chelating peptide derived from hairtail fish protein and preparation method and application thereof

A fish protein and iron chelation technology, which is applied in the preparation method of peptide, food preparation, peptide/protein composition, etc., can solve the problems of difficulty in passing through intestinal mucosa, large molecular weight of iron chelated protein, and unfavorable absorption of iron ions.

Active Publication Date: 2015-06-17
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although macromolecular proteins can also bind to iron ions, iron-chelating proteins have a large molecular weight and are difficult to pass through the intestinal mucosa, which is not conducive to the absorption of iron ions

Method used

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  • Iron chelating peptide derived from hairtail fish protein and preparation method and application thereof
  • Iron chelating peptide derived from hairtail fish protein and preparation method and application thereof
  • Iron chelating peptide derived from hairtail fish protein and preparation method and application thereof

Examples

Experimental program
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Embodiment

[0023] Examples: 1) Pretreatment of hairtail meat: Washed, boned and skinned hairtail ( Trichiurus japonicus ) Fish meat was put into the buffer according to the solid-liquid ratio of 1 g: 3mL, processed into a homogenate with a high-speed tissue masher, and then put into ether for 5 h according to the volume ratio of 1: 5mL, 4 ℃, 6000 rpm, The ether was removed by centrifugation for 15 min, and the precipitate was freeze-dried to obtain the defatted octopus meat.

[0024] 2) Enzymatic hydrolysis of hairtail meat protein: Add phosphate buffer (0.02 mol / L, pH 6.5) to the defatted hairtail meat according to the solid-liquid ratio of 1 g: 25 mL, and add papain (enzyme activity ≥ 2.0×10 4 U / g), after enzymatic hydrolysis at 60°C for 6 h, the solution was heated to 95°C and kept at this temperature for 10 min, then cooled to 37°C; the pH of the solution was adjusted to 8.0 with NaOH, according to the protein content of 2.0 % Add trypsin to the solution (enzyme activity ≥ 2.5...

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Abstract

The invention relates to a preparation method of iron chelating peptide derived from hairtail fish protein. The preparation method concretely comprises the following steps: taking hairtail fish as a raw material, carrying out enzymolysis for the hairtail fish protein with papain and trypsin, carrying out ultrafiltration, macroporous resin column chromatography, gel column chromatography and reversed-phase high performance liquid chromatography separation and purification, and thus obtaining the iron chelating peptide Ala-Pro-Pro-Glu-Asn-Gly-Met-Ala-Gln-Met, wherein the ESI / MS determining molecular weight is 1045.18Da. Due to the unique chelating and transport mechanism, the iron chelating peptide of hairtail fish protein is high in absorption availability, can simultaneously supplement polypeptide / amino acid and iron, and is an ideal iron supplement substance.

Description

technical field [0001] The invention relates to an iron chelating peptide derived from hairtail meat protein and a preparation method thereof, belonging to the technical field of deep processing of aquatic products. Background technique [0002] Biologically active peptides refer to those peptides with special physiological activities, which are the hotspots of current drug and health product research. At present, the active peptides produced by proteolysis mainly come from terrestrial protein sources. The living environment of marine organisms is obviously different from that of terrestrial environments. The amino acid composition or sequence of their proteins is very different from that of terrestrial biological proteins. It is also far more than terrestrial protein resources. Therefore, by referring to the enzyme engineering technology developed by terrestrial active peptides, using marine biological protein resources as raw materials, and through comprehensive researc...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12P21/06C07K1/36C07K1/34C07K1/20C07K1/16A61K38/08A61P3/02A23L1/29A23L1/305A23L33/165A23L33/18
Inventor 王斌迟长凤邓尚贵孙坤来陈荫
Owner ZHEJIANG OCEAN UNIV
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