Preparation methods of compound fermenting microbial agent and water-soluble fish protein amino acid fertilizer

A technology of composite fermentation bacteria and fish protein, applied in the preparation of organic fertilizers, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of easy pollution of the environment, corruption, and volatility, and achieve low cost, The effect of short production cycle and stable performance

Active Publication Date: 2018-10-26
宁波吉丰生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the above-mentioned patents do not use salt preservation, there are still the following problems: 1. The suitable temperature for protease to decompose protein is 55-65°C, while the heat-resistant temperature of bacteria such as yeast and lactobacillus is below 50°C
When the air is introduced and stirred, the material will be contaminated due to contact with external microorganisms, and the raw protein, amino acid and other nitrogen-containing substances are easily decomposed into ammonia, dimethylamine, indole, hydrogen sulfide, Histamine and other small molecular products are spoiled; at the same time, these small molecular products are odorous and volatile, which makes the fermentation process easy to pollute the environment

Method used

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  • Preparation methods of compound fermenting microbial agent and water-soluble fish protein amino acid fertilizer
  • Preparation methods of compound fermenting microbial agent and water-soluble fish protein amino acid fertilizer
  • Preparation methods of compound fermenting microbial agent and water-soluble fish protein amino acid fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Take Bacillus subtilis or Bacillus licheniformis 60% (weight ratio, live bacteria content ≥ 100 × 108CFU / g); Lactobacillus acidophilus or plantarum 20% (weight ratio, live bacteria content ≥ 100 × 108CFU / g ); Bifidobacterium bifidum or Bifidobacterium breve 20% (weight ratio, live bacteria content ≥ 100 * 108CFU / g). The above three kinds of bacteria are mixed to form a composite fermentation bacterial agent;

[0029] 2. Get corn starch 10% (weight ratio); Ammonium tartrate 5% (weight ratio); Disodium hydrogen phosphate 1% (weight ratio); Glucose 5% (weight ratio); Composite fermentation bacteria agent 3% (weight ratio) ; Water 76% (by weight). Stir the above raw materials evenly, control the temperature at 30°C, and activate the composite fermentation agent for 5 hours.

[0030] 3. Grinding the low-value fish and aquatic product processing waste into a homogenate with a pulverizer, after removing impurities, measure and add it to the fermenter; add the activated co...

Embodiment 2

[0034]1. Get 70% of Bacillus subtilis or Bacillus megaterium (weight ratio, live bacteria content ≥ 100 × 108CFU / g); Lactobacillus acidophilus or Lactobacillus brevis 15% (weight ratio, live bacteria content ≥ 100 × 108CFU / g ); Bifidobacterium bifidum or Bifidobacterium longum 15% (weight ratio, live bacteria content ≥ 100 * 108CFU / g). The above three kinds of bacteria are mixed to form a composite fermentation bacterial agent.

[0035] 2. Get corn starch 15% (weight ratio); Ammonium tartrate 3% (weight ratio); Disodium hydrogen phosphate 0.5% (weight ratio); Glucose 5% (weight ratio); Composite fermentation bacteria agent 2.5% (weight ratio) ; Water 74% (by weight). Stir the above raw materials evenly, control the temperature at 32°C, and activate the composite fermentation agent for 3 hours.

[0036] 3. Grinding the low-value fish and aquatic product processing waste into a homogenate with a pulverizer, after removing impurities, measure and add it to the fermenter; add th...

Embodiment 3

[0040] 1. Get Bacillus subtilis or Bacillus megaterium 65% (weight ratio, live bacteria content ≥ 100 × 108CFU / g); Lactobacillus acidophilus or Lactobacillus casei 25% (weight ratio, live bacteria content ≥ 100 × 108CFU / g ); Bifidobacterium bifidum or Bifidobacterium longum 10% (by weight, live bacteria content ≥ 100 × 108 CFU / g). The above three kinds of bacteria are mixed to form a composite fermentation bacterial agent.

[0041] 2. Get corn starch 8% (weight ratio); Ammonium tartrate 2% (weight ratio); Disodium hydrogen phosphate 0.8% (weight ratio); Glucose 4% (weight ratio); Composite fermentation bacteria agent 1.2% (weight ratio) ; Water 84% (by weight). Stir the above raw materials evenly, control the temperature at 35°C, and activate the bacteria for 3 hours.

[0042] 3. Use a pulverizer to pulverize low-value fish and aquatic product processing waste into a homogenate, remove impurities and add it to the fermenter; add the activated composite bacterial agent to the...

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Abstract

The invention discloses preparation methods of a compound fermenting microbial agent and a water-soluble fish protein amino acid fertilizer. The preparation methods include: 1) mixing facultative anaerobic bacillus, aerotolerant anaerobic lactobacillus and obligate anaerobic Bifidobacterium to form the compound fermenting microbial agent; 2) mixing corn starch, ammonium tartrate, disodium hydrogenphosphate, glucose, the compound fermenting microbial agent and water, stirring evenly, and activating the compound fermenting microbial agent; 3) smashing fish, shellfish, aquatic product processingleftovers into homogenate, removing impurities, quantitatively adding the homogenate into a fermentation tank, adding the activated compound fermenting microbial agent, which accounts for 0.2-5wt% ofthe homogenate, into the fermentation tank, mixing the activated compound fermenting microbial agent and the homogenate evenly, sealing the fermentation tank, and standing at normal temperature for 10-15 days to obtain fermented mash; 4) filtering the fermented mash with a filter to obtain fermented liquid which is fish protein amino acid fermented liquid; 5) adding at least one of following microelements including copper, iron, manganese, zinc, boron and molybdenum to prepare the required water-soluble fish protein amino acid fertilizer.

Description

technical field [0001] The present invention relates to the technical field of production technology of fermented bacterial agent and water-soluble fertilizer, in particular to a compound fermented bacterial agent and a method of using fish and other aquatic products and processing leftovers as raw materials, using biological fermentation technology to prepare fish protein amino acids and making water-soluble fertilizer . Background technique [0002] The problems of fertilizer loss, eutrophication of water body, destruction of soil structure and decline of soil fertility caused by the use of traditional fertilizers are more prominent. The application of organic fertilizer can increase the content of soil organic matter and improve the soil structure, which is an important part of the development of green agriculture and organic agriculture. With the development of my country's marine fishery and aquatic product processing industry, some huge and low-value fish and aquatic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/00C05F17/00C12R1/125C12R1/10C12R1/11C12R1/23C12R1/25C12R1/24C12R1/245C12R1/01
CPCC12N1/20C05B7/00C05G3/00C05F17/20C05C3/00C05F1/002C05F1/005C05F11/00C05F11/08C05G5/20Y02W30/40
Inventor 周雄黄吉人
Owner 宁波吉丰生物科技发展有限公司
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