Defatted soybean protein small peptide zinc chelate and preparation method thereof

A technology for the chelation of defatted soybean protein and small peptides, which is applied in the field of feed additives, can solve the problems of difficult practical application, high price, shortage of amino acid raw materials, etc., achieve high chelation rate, ensure chelation rate, and simple preparation process

Inactive Publication Date: 2018-07-06
OMEGA BIOTECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the shortage of amino acid raw materials and the high price, it is very difficult to apply in practice.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A kind of defatted soybean protein small peptide chelated zinc, its preparation method comprises the following steps: the preparation method of above-mentioned defatted soybean protein small peptide chelated zinc comprises the following preparation steps:

[0033] (1) Enzymolysis: enzymatically hydrolyze defatted soybean protein with compound protease, which includes alkaline protease, papain and flavor protease;

[0034] (2) Chelation: prepare defatted soybean protein small peptide chelated zinc aqueous solution;

[0035] (3) Drying: The defatted soybean protein small peptide chelated zinc product is obtained by spray drying.

[0036] The enzymatic hydrolysis process of the above step (1) is divided into the following steps:

[0037] (a) Dissolve 10 kg of defatted soybean protein powder in 35 kg of drinking water, and then adjust the pH value of the mixed slurry to 6.5;

[0038] (b) Add compound protease to the slurry in step (a) for enzymolysis reaction. The amount ...

Embodiment 2

[0062] A kind of defatted soybean protein small peptide chelated zinc, its preparation method comprises the following steps: the preparation method of above-mentioned defatted soybean protein small peptide chelated zinc comprises the following preparation steps:

[0063] (1) Enzymolysis: enzymatically hydrolyze defatted soybean protein with compound protease, which includes alkaline protease, papain and flavor protease;

[0064] (2) Chelation: prepare defatted soybean protein small peptide chelated zinc aqueous solution;

[0065] (3) Drying: The defatted soybean protein small peptide chelated zinc product is obtained by spray drying.

[0066] The enzymatic hydrolysis process of the above step (1) is divided into the following steps:

[0067] (a) Dissolve 10 kg of defatted soybean protein powder in 40 kg of drinking water, and then adjust the pH value of the mixed slurry to 7.0;

[0068] (b) Add compound protease to the slurry in step (a) for enzymolysis reaction. The amount ...

Embodiment 3

[0092] A kind of defatted soybean protein small peptide chelated zinc, its preparation method comprises the following steps: the preparation method of above-mentioned defatted soybean protein small peptide chelated zinc comprises the following preparation steps:

[0093] (1) Enzymolysis: enzymatically hydrolyze defatted soybean protein with compound protease, which includes alkaline protease, papain and flavor protease;

[0094] (2) Chelation: prepare defatted soybean protein small peptide chelated zinc aqueous solution;

[0095] (3) Drying: The defatted soybean protein small peptide chelated zinc product is obtained by spray drying.

[0096] The enzymatic hydrolysis process of the above step (1) is divided into the following steps:

[0097] (a) Dissolve 10 kg of defatted soybean protein powder in 45 kg of drinking water, and then adjust the pH value of the mixed slurry to 7.5;

[0098] (b) Add compound protease to the slurry in step (a) for enzymolysis reaction. The amount ...

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Abstract

The invention discloses a defatted soybean protein small peptide zinc chelate and a preparation method thereof. Most small peptides of the chelated zinc are dipeptides and tripeptides; the content ofthe small peptides with the molecular weight of 440 Da or below is 80 percent; the molecular weight of 100 percent of the small peptides is 1,000 Da or below; and the chelation rate of zinc is 98 percent or above. The preparation method comprises enzymolysis, chelation and drying. The preparation process disclosed by the invention is simple; the raw materials are low in cost and easy to obtain; the drying is performed by adopting a spray drying method, so that the production period is greatly shortened, and the time cost and the production cost are reduced; and the defatted soybean protein small peptide zinc chelate is suitable for large-scale production. Protein small peptides prepared by the preparation method are enzymolyzed by adopting compound protease, so that the enzymolysis is thorough; and the chelation rate of the prepared small peptide zinc chelate is more than or equal to 98 percent. When the defatted soybean protein small peptide zinc chelate prepared by the preparation method disclosed by the invention is used in an animal feed additive, the effect is obviously better than the effects of zinc sulfate and amino acid zinc chelate; and the defatted soybean protein smallpeptide zinc chelate is high in stability, high in absorption rate and high in utilization rate, and reduces pollution caused by animal metabolism to the environment.

Description

technical field [0001] The invention belongs to the technical field of feed additives, and specifically relates to a defatted soybean protein hydrolyzed small peptide chelated zinc and a preparation method thereof. Background technique [0002] Peptides are intermediate products produced by organisms in the process of degrading proteins, and are compounds composed of two or more amino acids connected by peptide bonds. The number of amino acid residues is less than 50 called polypeptides, 2 to 10 amino acids are called oligopeptides, and oligopeptides containing 2 or 3 amino acid residues are called small peptides. The average molecular weight of small peptides is about 300 Daltons, which can be completely absorbed into the animal circulatory system and directly metabolized by tissues, playing an important role in animal nutrition and metabolism. Small peptides have special nutritional and physiological functions. Due to their high absorption, high conversion rate and immune...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/107C07K1/34
CPCC07K1/107C07K1/34C12P21/06
Inventor 曹冬梅孙安权
Owner OMEGA BIOTECH SHANGHAI
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