Preparation process for zinc fish-skin collagen polypeptide chelate

A technology of fish skin collagen and preparation process, which is applied in the field of preparation process of fish skin collagen polypeptide chelated zinc, can solve the problem of low chelation rate and chelate yield, high chelation rate and chelate yield, The chelation process is not easy to control, etc., to achieve the effect of low cost, high chelation rate, and stable pH value

Active Publication Date: 2014-03-12
OCEAN UNIV OF CHINA
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The present invention aims to solve the problems that the chelation process is difficult to control, the chelation rate and chelate yield are low, and the chelation time is long when preparing fish skin collagen polypeptide chelated zinc in the prior art, and provides a fish skin collagen The preparation process of protein polypeptide chelated zinc has simple steps and can effectively control the chelation process, making the chelation process more stable, the chelation time is short, and the chelation rate and chelate yield are high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Weigh 27 mg of fish skin collagen polypeptide and dissolve it in water, adjust the pH to 7.2 to obtain a polypeptide solution, and the mass percentage of fish skin collagen polypeptide in the polypeptide solution is 40%.

[0043] (2) Add NaHCO with a mass concentration of 0.4% to the peptide solution 3 solution, chelating solution, NaHCO 3 The amount of solution added is 1% of the mass of the polypeptide solution.

[0044] (3) After raising the temperature of the chelating solution to 42°C, add 24.77mg of zinc sulfate (Zn in zinc sulfate 2+ The mass ratio of fish skin collagen peptide to fish skin collagen peptide is 1:2.7), and the reaction solution was obtained after the heat preservation reaction under ultrasonic conditions for 10 minutes. During this period, neutral gas was constantly blown into the chelating solution. 260W, the neutral gas is nitrogen, and the flow rate is 0.3 m 3 / h.

[0045] (4) After filtering the reaction solution, concentrate it to 30%...

Embodiment 2

[0050] (1) Dissolve 28 mg of fish skin collagen polypeptide in water, adjust the pH to 7.3 to obtain a polypeptide solution, and the mass percentage of fish skin collagen polypeptide in the polypeptide solution is 50%.

[0051] (2) Add NaHCO with a mass concentration of 0.2% to the peptide solution 3 solution, chelating solution, NaHCO 3 The amount of the solution added is 0.7% by mass of the polypeptide solution.

[0052] (3) After raising the temperature of the chelating solution to 45°C, add 20.92 mg of zinc chloride (Zn in zinc chloride 2+ The mass ratio of fish skin collagen peptide to fish skin collagen peptide is 1:2.8), and the reaction solution was obtained after the heat preservation reaction under ultrasonic conditions for 15 minutes. During this period, neutral gas was constantly blown into the chelating solution. 200W, the neutral gas is hydrogen, and the flux is 0.4 m 3 / h.

[0053] (4) After filtering the reaction solution, concentrate it to 40% of the origi...

Embodiment 3

[0057] (1) Weigh 29 mg of fish skin collagen polypeptide and dissolve it in water, adjust the pH to 7.4 to obtain a polypeptide solution, and the mass percentage of fish skin collagen polypeptide in the polypeptide solution is 60%.

[0058] (2) Add NaHCO with a mass concentration of 0.23% to the peptide solution 3 solution, chelating solution, NaHCO 3 The amount of solution added is 0.5% by mass of the polypeptide solution.

[0059] (3) After raising the temperature of the chelating solution to 40°C, add 24.77mg of zinc sulfate (Zn in zinc sulfate 2+ The mass ratio of fish skin collagen peptide to fish skin collagen peptide is 1:2.9), and the reaction liquid was obtained after the heat preservation reaction under ultrasonic conditions for 12 minutes. During this period, neutral gas was constantly blown into the chelating liquid. 300W, the neutral gas is nitrogen, and the flow rate is 0.5 m 3 / h.

[0060] (4) After filtering the reaction solution, concentrate it to 37% of t...

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PUM

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Abstract

The invention discloses a preparation process for a zinc fish-skin collagen polypeptide chelate to overcome the problems of difficultly controllable chelating process, a low chelating rate, low chelate yield and long chelating time in conventional preparation processes for the zinc fish-skin collagen polypeptide chelate. The process provided by the invention optimizes chelation reaction conditions, adds a NaHCO3 solution in chelating process at the same time so as to maintain stability of the pH value of a reaction system, cooperatively uses ultrasonic treatment to improve chelating efficiency and chelate yield and introduces air in chelating process air to maintain stability of chelation reaction temperature. The preparation process in the invention has the advantages of simple steps, capacity of effectively controlling the chelating process and allowing the chelating process to be more stable, short chelating time, a high chelating rate and high chelate yield.

Description

technical field [0001] The invention relates to the technical field of deep processing of aquatic products, in particular to a preparation process of fish skin collagen polypeptide chelated zinc. [0002] Background technique [0003] As an essential trace element for the human body, zinc has the functions of participating in the composition of many metalloenzymes in the human body, promoting the growth and development of the body, and tissue regeneration. However, ingredients such as phosphate and oxalic acid in food will interfere with the absorption of zinc, and the absorption and utilization rate of inorganic zinc and organic zinc in zinc supplementation drugs are also low. Therefore, finding a safe and effective zinc supplement product is of great significance to human health. [0004] In vitro, zinc is chelated with small peptides to form a trace element chelate with amino acids and small peptides as the main ligands, which can be operated in the form of a whole, wit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C07K1/30
CPCC07K14/78
Inventor 杨会成李八方董士远侯虎赵雪
Owner OCEAN UNIV OF CHINA
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