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Bacillus cereus and application thereof

A Bacillus cereus, seed technology, applied in applications, bacteria, animal feed, etc., can solve the problems of water environment deterioration, chemical oxygen demand, biological oxygen demand exceeding the standard, incomplete purification, etc., to promote healthy development, The effect of enhancing disease resistance and enhancing immunity

Active Publication Date: 2013-01-30
QINGDAO VLAND BIOTECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The feeding-based breeding mode causes residual bait, feces, residues of dead organisms, sediments from the bottom of the breeding pond, nitrogen, phosphorus and other eutrophication factors to be discharged into the water body, causing the chemical oxygen demand, The biological oxygen demand has seriously exceeded the standard, and the water environment is deteriorating, resulting in frequent occurrence of aquatic animal diseases
Traditional methods of purification of the breeding environment such as mechanical dredging, chemical degradation, and water change have shortcomings such as incomplete purification, easy to produce secondary pollution, and endanger the breeding function.

Method used

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  • Bacillus cereus and application thereof
  • Bacillus cereus and application thereof
  • Bacillus cereus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Screening and Identification of Example 1 Bacterial Strains

[0018] 1. Screening

[0019] (1) Take 50 mL of water samples from the aquaculture ponds of Shazikou aquaculture farmers in Laoshan District, Qingdao City, and bathe in 80°C water for 15 minutes to kill non-spore bacteria;

[0020] (2) After dilution, spread the water sample on nutrient agar medium (peptone 10.0g / L, beef extract powder 3.0g / L, sodium chloride 5.0g / L, agar 15.0g / L) plate, 37℃, Cultivate for 24 hours;

[0021] (3) Select typical Bacillus colonies to streak on the nutrient agar medium plate, culture at 37°C for 24 hours;

[0022] 2. Identification

[0023] Through the observation of strain morphology and the analysis of physical and chemical indicators, 20 strains were selected for the laboratory aquaculture wastewater treatment experiment. The experimental water body was 1000mL, and the ammonia nitrogen in the water before treatment was 0.966±0.017mg / L, and the nitrite nitrogen was 0.815±0.10...

Embodiment 2

[0031] The preparation of embodiment 2 bacillus cereus microbial preparation

[0032] 1. Preparation of slant strains: Streak inoculation of the above-mentioned Bacillus cereus on the nutrient agar medium, culture at 37°C for 12 hours;

[0033] 2. Preparation of solid seeds: Inoculate the slant strain prepared in step 1 to the fresh nutrient agar slant of the eggplant bottle, culture at 37°C for 12 hours;

[0034] 3. Preparation of liquid seeds: Wash the solid seeds prepared in step 2 from the slope of the eggplant bottle and inoculate them into the seed tank, culture at 37°C for 12 hours, the components of the medium in the seed tank and their weight ratios are: bean cake powder 2.5 %, corn steep liquor 2%, disodium hydrogen phosphate 0.1%, ammonium sulfate 0.05%, manganese sulfate 0.05%;

[0035] 4. Fermentation culture: inoculate the seeds in the seed tank into a 5-ton fermenter for cultivation, the inoculation amount is 5%, 37°C, 150rpm, and cultivate for 20h. The compone...

Embodiment 3

[0039] Application of embodiment 3 bacillus cereus microbial preparation

[0040] 1. The whole pool is splashed

[0041] After mixing the Bacillus cereus microbial preparation prepared in Example 2 with water, sprinkle the shrimp ponds in the whole pond. The dosage per mu (1 meter of water depth) is 0.5-1 kg. Sprinkle once every 10 days, continuously for 3 times, and detect the water body The content of ammonium nitrogen substance, nitrite nitrogen and COD in medium, concrete data are as shown in table 2:

[0042] Table 2 water quality parameters before and after treating shrimp ponds with SC2 preparation

[0043]

[0044] As can be seen from the table, after the Bacillus cereus of the present invention is processed, the degradation rates of ammonia nitrogen substances, nitrite nitrogen and COD in the water body of shrimp ponds reach 65%, 85% and 67% respectively, illustrating that the wax of the present invention Bacillus spp. SC2 can degrade ammonia nitrogen, nitrite ni...

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Abstract

The invention relates to a strain of bacillus cereus SC2 with the collection number of CGMCC No.3836. The strain of the bacillus cereus SC2 has an obvious water purifying function, three days after sewage is treated by the bacillus cereus SC2 strain, the removal rates of the ammonia nitrogen, nitrite nitrogen and chemical oxygen demand (COD) in the sewage are 70.15%, 94.12% and 43.12% respectively, the pH value in the sewage is stabilized, the dissolved oxygen in the sewage is increased, and the immunity and disease resistance of aquatic animals are enhanced, so that the healthy development of the aquaculture industry is promoted.

Description

technical field [0001] The invention belongs to the technical field of water body purification microorganisms, and in particular relates to a strain of bacillus cereus and its application. Background technique [0002] In recent years, my country's aquaculture industry has developed vigorously. However, the aging and degradation of cultured species, the frequent occurrence of aquatic animal diseases, and the deteriorating environment of aquaculture water bodies have become the bottlenecks hindering the development of my country's aquaculture industry. Especially the deteriorating environment of the aquaculture water body has brought huge economic losses to the aquaculture industry. The feeding-based breeding mode causes residual bait, feces, residues of dead organisms, sediments from the bottom of the breeding pond, nitrogen, phosphorus and other eutrophication factors to be discharged into the water body, causing the chemical oxygen demand, The biological oxygen demand has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34A23K1/16C12R1/085
Inventor 彭虹旎王增元王霁昀陈营
Owner QINGDAO VLAND BIOTECH GRP
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